Good advice Re: [Histonet] Filter paper

[Gayle Callis]

RE: [Histonet] Filter paperAlso, this caveat applies to coffee filters too. 

Gayle M. Callis 
HTL,TH,MT(ASCP)
  ----- Original Message ----- 
  From: Bonner, Janet 
  To: Wellborn, Janci R ; Weems, Joyce ; godsgalnow <@t> aol.com ; Gayle Callis ; histonet-bounces <@t> lists.utsouthwestern.edu ; Mylène de Champlain ; histonet <@t> lists.utsouthwestern.edu 
  Sent: Monday, July 28, 2008 7:29 AM
  Subject: RE: [Histonet] Filter paper


  Watch out that the paper you are using does not change the acidity of your solution (we had a problem with that once using the paper towels!

  Janet L. Bonner, HTL (ASCP)
  Pathology Laboratory
  Florida Hospital Winter Park
  janet.bonner <@t> FLHOSP.org
  407-646-7559


------------------------------------------------------------------------------
  From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Wellborn, Janci R
  Sent: Fri 7/25/2008 10:37 AM
  To: Weems, Joyce; godsgalnow <@t> aol.com; Gayle Callis; histonet-bounces <@t> lists.utsouthwestern.edu; Mylène de Champlain; histonet <@t> lists.utsouthwestern.edu
  Subject: RE: [Histonet] Filter paper


  Both help to keep costs down . . .

  -----Original Message-----
  From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Weems, Joyce
  Sent: Thursday, July 24, 2008 5:45 PM
  To: godsgalnow <@t> aol.com; Gayle Callis; histonet-bounces <@t> lists.utsouthwestern.edu; Mylène de Champlain; histonet <@t> lists.utsouthwestern.edu
  Subject: RE: [Histonet] Filter paper

  And paper towels will work when you have run out of filter paper...

  -----Original Message-----
  From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of godsgalnow <@t> aol.com
  Sent: Thursday, July 24, 2008 5:17 PM
  To: Gayle Callis; histonet-bounces <@t> lists.utsouthwestern.edu; Mylène de Champlain; histonet <@t> lists.utsouthwestern.edu
  Subject: Re: [Histonet] Filter paper

  I sometimes use coffee filters.
  Roxanne
  ------Original Message------
  From: Gayle Callis
  Sender: histonet-bounces <@t> lists.utsouthwestern.edu
  To: Mylène de Champlain
  To: histonet <@t> lists.utsouthwestern.edu
  Subject: Re: [Histonet] Filter paper
  Sent: Jul 24, 2008 4:22 PM

  What is important is not the number so much as if the paper is high retention and what is filtering speed.  Whatman #4, if I remember correctly, is a medium fast paper, and should be  ideal for filtering staining solution.  Somewhere in the passage of time, I was told not to use paper that was really slow filtering speed and high retention for filtering dye solutions as the paper may retain too much of the dye needed. Now that could be an old wife's tale, but to this day I always use a medium fast paper.

    I also purchase something other than Whatman, as companies sell
  equivalents for a much cheaper price.  Whatman 54 should also work.   Big
  companies like ThermoScientific give the equivalents for their brand as compared to Whatman papers and are certainly more economical.

  Gayle M. Callis
  HTL/HT/MT(ASCP)


  ----- Original Message -----
  From: "Mylène de Champlain" <myl__28 <@t> hotmail.com>
  To: <histonet <@t> lists.utsouthwestern.edu>
  Sent: Wednesday, July 23, 2008 4:31 PM
  Subject: [Histonet] Filter paper



  Hi,

    To filter blue celestin, is what I have to use a filter paper number 1 or 4? Thank!

    Mylène de Champlain
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