[Histonet] RNA isolation from tissue

shehnaz khan shehnazster <@t> gmail.com
Sun Jul 27 12:00:07 CDT 2008


Can anyone help / offer any suggestions on extracting RNA from fresh frozen
breast tissue.   Following the usual cleaning, wiping down with RNAse Away /
Zap etc.

We're using Trizol Reagent to store / collect  samples in and then
immediately snap freeze.  RNA is isolated using the Trizol method.   The RNA
is severely degraded.

Please Help!

S. Khan


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