[Histonet] PAX-2

[Patti Loykasek]

Hi Thomas. Zymed has a Pax-2 concentrate, I don't know if they have a
pre-dilute available. We use it on paraffin embedded human tissue with a
heat citrate pretreatment. I always think it best to determine your own
optimal titer - so variable depending on fixation, processing, pretreatment,
detection, etc.... We always start with the manufacturers recommendation &
do a 'sandwich titer'. For example, if recommended is 1:100, we do 1:50,
1:100, & 1:250. Hope this helps.


Patti Loykasek BS, HTL, QIHC
PhenoPath Laboratories
Seattle, WA





> Hey there folks,
> 
> Anyone running PAX-2?  I've had no luck finding it as a pre-dilute and
> have only found one vendor with a concentrate.  So...anyone know of a
> pre-dilute out there and are you running it?  Or if you're running it as
> a concentrate, what is the titration?  Human clinical laboratory
> application is what I'm interested in.  Thanks in advance for any
> assistance here.
> 
> Thomas Jasper HT (ASCP) BAS
> Histology Supervisor
> Central Oregon Regional Pathology Services
> Bend, OR 97701
> 541/693-2677
> tjasper <@t> copc.net
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 



This e-mail message, including any attachments, is for the sole use of the 
intended recipients and may contain privileged information. Any unauthorized 
review, use, disclosure or distribution is prohibited. If you are not the intended 
recipient, please contact the sender by e-mail and destroy all copies of the 
original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. 
at (206) 374-9000.