[Histonet] Filter paper

[Wellborn, Janci R]

Both help to keep costs down . . . 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Weems, Joyce
Sent: Thursday, July 24, 2008 5:45 PM
To: godsgalnow <@t> aol.com; Gayle Callis; histonet-bounces <@t> lists.utsouthwestern.edu; Mylène de Champlain; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Filter paper

And paper towels will work when you have run out of filter paper...

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of godsgalnow <@t> aol.com
Sent: Thursday, July 24, 2008 5:17 PM
To: Gayle Callis; histonet-bounces <@t> lists.utsouthwestern.edu; Mylène de Champlain; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Filter paper

I sometimes use coffee filters. 
Roxanne
------Original Message------
From: Gayle Callis
Sender: histonet-bounces <@t> lists.utsouthwestern.edu
To: Mylène de Champlain
To: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Filter paper
Sent: Jul 24, 2008 4:22 PM

What is important is not the number so much as if the paper is high retention and what is filtering speed.  Whatman #4, if I remember correctly, is a medium fast paper, and should be  ideal for filtering staining solution.  Somewhere in the passage of time, I was told not to use paper that was really slow filtering speed and high retention for filtering dye solutions as the paper may retain too much of the dye needed. Now that could be an old wife's tale, but to this day I always use a medium fast paper.

  I also purchase something other than Whatman, as companies sell 
equivalents for a much cheaper price.  Whatman 54 should also work.   Big 
companies like ThermoScientific give the equivalents for their brand as compared to Whatman papers and are certainly more economical.

Gayle M. Callis
HTL/HT/MT(ASCP)


----- Original Message -----
From: "Mylène de Champlain" <myl__28 <@t> hotmail.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, July 23, 2008 4:31 PM
Subject: [Histonet] Filter paper



Hi,

  To filter blue celestin, is what I have to use a filter paper number 1 or 4? Thank!

  Mylène de Champlain
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