[Histonet] Filter paper

[Gayle Callis]

What is important is not the number so much as if the paper is high 
retention and what is filtering speed.  Whatman #4, if I remember correctly, 
is a medium fast paper, and should be  ideal for filtering staining 
solution.  Somewhere in the passage of time, I was told not to use paper 
that was really slow filtering speed and high retention for filtering dye 
solutions as the paper may retain too much of the dye needed. Now that could 
be an old wife's tale, but to this day I always use a medium fast paper.

  I also purchase something other than Whatman, as companies sell 
equivalents for a much cheaper price.  Whatman 54 should also work.   Big 
companies like ThermoScientific give the equivalents for their brand as 
compared to Whatman papers and are certainly more economical.

Gayle M. Callis
HTL/HT/MT(ASCP)


----- Original Message ----- 
From: "Mylène de Champlain" <myl__28 <@t> hotmail.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, July 23, 2008 4:31 PM
Subject: [Histonet] Filter paper



Hi,

  To filter blue celestin, is what I have to use a filter paper number 1 or 
4? Thank!

  Mylène de Champlain
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