[Histonet] anyone doing kidneys in general?

[Jennifer Sipes]

Hi everyone!
We're looking at the proximal tubules in mice and we're finding that they are either collapsed or whole looking but the edges are crinkled.  I'm perfusing the kidney, but I'm wondering if anyone had any suggestions on how to get nice whole looking proximal tubules?  Should I maybe perfuse more to ensure that they're opening up for us or is there another method?
Please let me know and thanks in advance for all your help!
Jennifer K. Sipes, ALAT
Sr. Laboratory Technician 
Johns Hopkins University 
Ross 929 and Ross 933
720 Rutland Avenue 
Baltimore, MD  21205 
phone:  410-614-0131 
               410-955-9688 
fax:         410-955-9677 
cell:        443-631-6361 
e-mail:  jsipes1 <@t> jhmi.edu