[Histonet] rat brain: glycerol vs sucrose cryoprotection / Infiltration with Neg-50 at room temperature before freezing

[Larvaron Pierre]

Dear all,

I am looking for a protocol to enhance the quality of my frozen sections 
of fixed rat brain. I have two questions:

- Has anyone used a 20% glycerol and 2%DMSO (in buffer or fixative) to 
cryoprotect tissue instead of 30% sucrose?
(http://www.histosearch.com/histonet/Aug05A/Re.Histonetglycerolcryopr.html)

- Has anyone used infiltration of tissue at room temperature  with 
medium for embedding and cryosectionning (Neg-50 for example) before 
freezing?
(http://www.ncbi.nlm.nih.gov/pubmed/8128478?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum)

Thanks a lot.

Pierre

-- 
Dr. Pierre Larvaron
Post-Doc
Service du Développement et de la Croissance
Département de l'Enfant et de l'Adolescent
Faculté de Médecine, Université de Genève
Avenue de la Roseraie 64
CH - 1205 Genève
tel: +41 22 382 4569
Fax: +41 22 347 5979