[Histonet] cryostat

Mon Jul 21 13:10:35 CDT 2008

Hi all, I am looking to buy a cryostat - I have now got a Bright 
Starlet 2212 with which I have had a lot of problems.  Does anyone have 
suggestions for a cryostat? We are a small research lab with limited 
funds.  Thanks - Melissa

Quoting histonet-request <@t> lists.utsouthwestern.edu:

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> Today's Topics:
>
>   1. Shirley Phua is out-of-office ... (Shirley PHUA)
>   2. Rapid Golgi (Hyunchul Lee)
>   3. waste from Sakura Xpress (Angela Bitting)
>   4. Re: Histonet Digest, Vol 56, Issue 23 (Patricia Karlisch)
>   5. (no subject) (MARY JOHNSON)
>   6. Albumin Antibody (Karen Lapanowski)
>   7. RE: AP Cerner Copath vs Sunquest Copath vs others
>      (Michael Mihalik)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Mon, 21 Jul 2008 02:04:15 +0800
> From: Shirley PHUA <Shirley_PHUA <@t> hsa.gov.sg>
> Subject: [Histonet] Shirley Phua is out-of-office ...
> To: histonet <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> 	<OFF84A7526.2B6BF7C6-ON4825748C.0063443A-4825748C.0063443A <@t> gems2.gov.sg>
>
> Content-Type: text/plain; charset=US-ASCII
>
> I will be out of the office from  21-07-2008 to 22-07-2008.
>
> I'll be away on course 21-22 July 2008.
>
> Pathologists:
> I will process your requests when I return. If urgent, please forward your
> email to Henry_Kyaw <@t> hsa.gov.sg
>
> ------------------------------
>
> Message: 2
> Date: Sun, 20 Jul 2008 23:39:46 -0700 (PDT)
> From: Hyunchul Lee <hlee <@t> medsci.usyd.edu.au>
> Subject: [Histonet] Rapid Golgi
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <204741.91719.qm <@t> web31405.mail.mud.yahoo.com>
> Content-Type: text/plain; charset=us-ascii
>
> Hello,
>
> I've been trying out this 'osmium-free' rapid Golgi protocol (not 
> Golgi-Cox), and it seems to work-
> However, I've been having a couple of problems...
>
> After impregnation with silver nitrate (0.75%) solution, I immersed 
> my brain in 30% sucrose for 24 hrs,
> then sectioned it on a freezing microtome.  Out of my eagerness, I 
> mounted a section and viewed it
> under a microscope- I was quite impressed!  As it was getting late, I 
> returned my sections to distilled water
> so that I could mount them the follwing day.
>
> Upon returning the next day, I mounted my sections.  I hadn't 
> coverslipped them yet...
> I viewed these again under the microscope and found that the staining 
> I had seen earlier was nowhere to be seen!
> All I could see were brown blotches roughly corresponding to where 
> the perikarya were.
> Google gave me the impression that others have been having similar 
> problems regarding the longevity of
> Golgi-stained sections.  Is there a way to stop this decomposition of 
> rapid-Golgi product?
> We don't have Canada-balsam though...
>
> I had been hoping to perform immunohistochemistry on these same 
> sections, and I have heard of people
> immunolabeling sections AFTER Golgi labeling, yet if the Golgi 
> product has such a short life-span in water,
> this would be impossible.  Is there a way to stop this decay in rapid 
> Golgi material?
>
> Thank you in advance,
>
>
> Hyunchul Lee
> PhD student
>
> Systems Neuroscience Laboratory
> N121 Anderson Stuart Bldg. (F13)
> The University of Sydney, NSW, 2006
> Email: hlee <@t> medsci.usyd.edu.au
>
>
>
>
> ------------------------------
>
> Message: 3
> Date: Mon, 21 Jul 2008 08:20:40 -0400
> From: "Angela Bitting" <akbitting <@t> geisinger.edu>
> Subject: [Histonet] waste from Sakura Xpress
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <488446D8.2B7F.00C9.0 <@t> geisinger.edu>
> Content-Type: text/plain; charset="us-ascii"
>
> How are folks disposing of their waste from the Sakura Xpress 
> processors? I welcome the vendors input on this too.
>
> Angela Bitting, HT(ASCP)
> Technical Specialist, Histology
> Geisinger Medical Center
> 100 N Academy Ave. MC 23-00
> Danville, PA 17822
> phone  570-214-9634
> fax  570-271-5916
>
> No trees were hurt in the sending of this email
> However many electrons were severly inconvienienced!
>
>
>
>
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> -------------- next part --------------
> BEGIN:VCARD
> VERSION:2.1
> X-GWTYPE:USER
> FN:Bitting, Angela
> TEL;WORK:570-271-6844
> ORG:;Histology
> EMAIL;WORK;PREF;NGW:AKBITTING <@t> geisinger.edu
> N:Bitting;Angela
> END:VCARD
>
>
> ------------------------------
>
> Message: 4
> Date: Mon, 21 Jul 2008 09:28:11 -0400
> From: "Patricia Karlisch" <Pkarlisch <@t> hmc.psu.edu>
> Subject: [Histonet] Re: Histonet Digest, Vol 56, Issue 23
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <488456AB.07F7.008C.0 <@t> hmc.psu.edu>
> Content-Type: text/plain; charset=US-ASCII
>
> Jason,
>   I agree with you about Air Transportation and their specific rules 
> governing Air Transport, but hospitals have different requirements in 
> transporting hazardous materials within a hospital, from lab to lab 
> or clinic site to lab or by courier to lab.  The secondary container 
> is for the protection of the transporter (hospital) and the specimen 
> integrity.
>   I may be reading the email wrong from Richard, but it sounded like 
> specimens were being transported in the equivalent of a zip lock bag. 
>  If I was wrong I apologise.
>   Pat Karlisch
>
> Pat Karlisch
> Supervisor, Histology, Pathology and Laboratory Medicine
> Penn State Milton S. Hershey Medical Center
> Mail Code H179
> Hershey, PA 17033
> Phone (717) 531-6072
> Fax: (717) 531- 7741
> email: pkarlisch <@t> psu.edu
>
> *****E-Mail Confidentiality Notice*****
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>>>> <histonet-request <@t> lists.utsouthwestern.edu> 7/20/2008 1:04 PM >>>
> Send Histonet mailing list submissions to
> histonet <@t> lists.utsouthwestern.edu
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> Today's Topics:
>
>   1. RE: Formalin in plastic bags (Burrill, Jason)
>   2. troubleshoot of positive controls for PKCalpha and eNOS
>      antibody (shazana hilda)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Sat, 19 Jul 2008 13:38:10 -0400
> From: "Burrill, Jason" <Jason.Burrill <@t> crl.com>
> Subject: [Histonet] RE: Formalin in plastic bags
> To: <histonet <@t> lists.utsouthwestern.edu>,<Pkarlisch <@t> hmc.psu.edu>
> Message-ID:
> <1AD4E907E9B6F648AEF1B3A20A9B0E1E013B8E43 <@t> shr-exch2.na01.crl.com>
> Content-Type: text/plain;charset="US-ASCII"
>
> Patricia you are correct about the classification of most formalin
> solutions being hazardous for air transport but as it relates to 10%
> neutral buffered formalin it does not apply.  IATA regulations, which
> govern air transports, clearly state in the Dangerous Goods Regulations
> that any solution containing less than 10% formaldehyde is not
> considered hazardous for air transport.  And since 10% neutral buffered
> formalin contains only 4% formaldehyde it is not considered a dangerous
> good for transport.  I will say that you should always check with your
> commercial carrier (e.g. FedEx, UPS) before final classification of any
> material that there is a question about as they may have stricter
> requirements.
>
>
>
> Now if you are speaking about the transport of Patient Specimens as
> defined by IATA, exempt human or animal, which formalin fixed tissue
> typically falls into then what is required is a leak-proof primary
> receptacle, leak-proof secondary packaging and a rigid outer packaging
> with size dimensions of no less than 100 mm x 100 mm.
>
>
>
> And my final disclaimer will be that you should always check with your
> EHS department to make sure that you are following your
> institutional/company policies.
>
>
>
> Regards,
>
> Jason
>
>
>
> Jason Burrill
>
> Sr. Manager, Histology and Laboratory Safety
>
> Research Animal Diagnostic Services
>
> Charles River
>
> 251 Ballardvale St
>
> Wilmington, MA 01887
>
> Office: 781-222-6152
>
> Fax: 978-988-8793
>
> jason.burrill <@t> crl.com
>
> www.criver.com <http://www.criver.com/>
>
>
>
> Accelerating Drug Development. Exactly.
>
>
>
> Notice - This email and any files transmitted with it are confidential
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>
>
>
> ------------------------------
>
>
>
> Message: 2
>
> Date: Fri, 18 Jul 2008 13:34:46 -0400
>
> From: "Patricia Karlisch" <Pkarlisch <@t> hmc.psu.edu>
>
> Subject: [Histonet] Formalin in plastic bags
>
> To: <Histonet <@t> lists.utsouthwestern.edu>
>
> Message-ID: <48809BF6.07F7.008C.0 <@t> hmc.psu.edu>
>
> Content-Type: text/plain; charset=US-ASCII
>
>
>
> Richard,
>
>   There are standards for sending specimens that are in a hazardous
> solution, like Formalin.  Plastic bags are secondary containers and
> should not be used to transport fluid. The specimens should be in a
> secure  plastic container that seals tightly and will not puncture
> causing injury to others.
>
>   Pat Karlisch
>
>
>
> Pat Karlisch
>
> Supervisor, Histology, Pathology and Laboratory Medicine
>
> Penn State Milton S. Hershey Medical Center
>
> Mail Code H179
>
> Hershey, PA 17033
>
> Phone (717) 531-6072
>
> Fax: (717) 531- 7741
>
> email: pkarlisch <@t> psu.edu
>
>
>
> *****E-Mail Confidentiality Notice*****
>
> This message (including any attachments) contains information intended
> for a specific individual(s) and purpose that may be privileged,
> confidential or otherwise protected from disclosure pursuant to
> applicable law.  Any inappropriate use, distribution or copying of the
> message is strictly prohibited and may subject you to criminal or civil
> penalty.  If you have received this transmission in error, please reply
> to the sender indicating this error and delete the transmission from
> your system immediately.
>
>
>
>
>
>
>
> ------------------------------
>
> Message: 2
> Date: Sat, 19 Jul 2008 19:56:28 -0700 (PDT)
> From: shazana hilda <hilda_1075 <@t> yahoo.com>
> Subject: [Histonet] troubleshoot of positive controls for PKCalpha and
> eNOSantibody
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <939284.12676.qm <@t> web38806.mail.mud.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Dear Histonetters,
>
> I'm having difficulties to obtain positive staining of positive 
> control for PKCalpha (H-7:sc-8393) and NOS3(c-20:sc-654) antibody. 
> Both antibody were purchased from SantaCruz Biotechnology Inc.and 
> used for IHC.Below are the methods that i used:
>
> 1)PKCalpha
>    -incubation time: 1hour,room temperature (24-25 celcius)
>    -Antibody dilution: 1:50
>    - Target Antigen Retrieval Buffer: Citrate Buffer, pH6
>    -Device: microwave (3x at 600W for 5min)
>    -Detection method: polymeric method by LabVision
> 2) NOS3 <@t> eNOS
>    -incubation time: 1hour,room temperature (24-25 celcius)
>    -Antibody dilution: 1:50
>    - Target Antigen Retrieval Buffer: Citrate Buffer, pH6
>    -Device: microwave (3x at 600W for 5min)
>    -Detection method: polymeric method by LabVision
>
> I've used normal rat kidney as suggested by few journals for positive 
> controls of both antibody. however my positive controls produced 
> negative staining.
>
> Your suggestion and quick response are highly appreciated
>
> Shazana Hilda Shamsuddin
> MSc.of Molecular Pathology,
> Dept. of Pathology,
> School of Medical Sciences,
> Universiti Sains Malaysia,
> 16150 Kubang Kerian,
> Kelantan, Malaysia.
> Email: hilda_1075 <@t> yahoo.com
>
>
>
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 56, Issue 23
> ****************************************
>
>
> ------------------------------
>
> Message: 5
> Date: Mon, 21 Jul 2008 07:59:35 -0700 (PDT)
> From: MARY JOHNSON <beingmary53 <@t> sbcglobal.net>
> Subject: [Histonet] (no subject)
> To: Histonet <@t> lists.utsouthwestern.edu
> Message-ID: <207645.64703.qm <@t> web81605.mail.mud.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Is there something wrong with the histonet? I have not received 
> anything from you far a while
>  my e-mail is beingmary53 <@t> sbcglobal.net Please check my e-mail address.
>  Thank you Mary
>
>
> ------------------------------
>
> Message: 6
> Date: Mon, 21 Jul 2008 11:38:10 -0400
> From: "Karen Lapanowski" <KLAPANO1 <@t> hfhs.org>
> Subject: [Histonet] Albumin Antibody
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <488475220200002800009336 <@t> gwia2v.net.hfh.edu>
> Content-Type: text/plain; charset=us-ascii
>
> Hello,
> Can anyone recommend a supplier and/or protocol for staining albumin 
> in fixed 100 micron vibratome section of rat brain?
> Thanks,
>
> Karen Lapanowski
> Henry Ford Health System
> Radiation Oncology
> 3065 E & R
> 313-916-9386
>
>
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> ------------------------------
>
> Message: 7
> Date: Mon, 21 Jul 2008 11:45:43 -0400
> From: "Michael Mihalik" <mike <@t> pathview.com>
> Subject: RE: [Histonet] AP Cerner Copath vs Sunquest Copath vs others
> To: "'Cristian Britos'" <cbritos <@t> pathsrv.com>,	"'Richard Cartun'"
> 	<Rcartun <@t> harthosp.org>, <gu.lang <@t> gmx.at>,
> 	<histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <031001c8eb48$d5c57780$81506680$@com>
> Content-Type: text/plain;	charset="US-ASCII"
>
> Good day,
>
> I wanted to wait a few days before I responded to this request as I
> represent a system vendor.   I would love to talk to you about our system
> and why your organization should consider us, but I suspect this would be
> best dealt with via direct email.
>
> To the group, I pose this question:
>
> I try to be very conscientious of the fact that this method of communication
> and interaction is intended to be primarily between histology personnel and
> that vendors should be very respectful of that idiom.  Do you think my offer
> above is appropriate?  Would you like to see our dialog?
>
> I'd appreciate any feedback that you can provide.
>
> Michael Mihalik
> PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968
>
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cristian
> Britos
> Sent: Thursday, July 17, 2008 3:26 PM
> To: Richard Cartun; gu.lang <@t> gmx.at; histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] AP Cerner Copath vs Sunquest Copath vs others
>
> Would someone be willing to share their experiences about Sunquest
> copath as their LIS system for pathology versus other systems?
> Needless to say, we are looking for options.... (currently we have
> cerner copath 3.0)
> Thanks,
> Cris
>
> _______________________________________________
> Histonet mailing list
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>
>
>
> ------------------------------
>
> _______________________________________________
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>
> End of Histonet Digest, Vol 56, Issue 24
> ****************************************
>

Melissa R. Mazan, DVM, Diplomate ACVIM
Associate Professor and Director of Equine Sports Medicine
Department of Clinical Sciences
Tufts Cumming School of Veterinary Medicine
200 Westborough Road
North Grafton, MA 01536
Tel:508-839-5395
Fax:508-839-7922
email: melissa.mazan <@t> tufts.edu