[Histonet] Re: Histonet Digest, Vol 56, Issue 23

Mon Jul 21 08:28:11 CDT 2008

Jason,
   I agree with you about Air Transportation and their specific rules governing Air Transport, but hospitals have different requirements in transporting hazardous materials within a hospital, from lab to lab or clinic site to lab or by courier to lab.  The secondary container is for the protection of the transporter (hospital) and the specimen integrity. 
   I may be reading the email wrong from Richard, but it sounded like specimens were being transported in the equivalent of a zip lock bag.  If I was wrong I apologise.  
   Pat Karlisch

Pat Karlisch
Supervisor, Histology, Pathology and Laboratory Medicine
Penn State Milton S. Hershey Medical Center
Mail Code H179
Hershey, PA 17033
Phone (717) 531-6072
Fax: (717) 531- 7741
email: pkarlisch <@t> psu.edu 

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Today's Topics:

   1. RE: Formalin in plastic bags (Burrill, Jason)
   2. troubleshoot of positive controls for PKCalpha and eNOS
      antibody (shazana hilda)

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Message: 1
Date: Sat, 19 Jul 2008 13:38:10 -0400
From: "Burrill, Jason" <Jason.Burrill <@t> crl.com>
Subject: [Histonet] RE: Formalin in plastic bags
To: <histonet <@t> lists.utsouthwestern.edu>,<Pkarlisch <@t> hmc.psu.edu>
Message-ID:
<1AD4E907E9B6F648AEF1B3A20A9B0E1E013B8E43 <@t> shr-exch2.na01.crl.com>
Content-Type: text/plain;charset="US-ASCII"

Patricia you are correct about the classification of most formalin
solutions being hazardous for air transport but as it relates to 10%
neutral buffered formalin it does not apply.  IATA regulations, which
govern air transports, clearly state in the Dangerous Goods Regulations
that any solution containing less than 10% formaldehyde is not
considered hazardous for air transport.  And since 10% neutral buffered
formalin contains only 4% formaldehyde it is not considered a dangerous
good for transport.  I will say that you should always check with your
commercial carrier (e.g. FedEx, UPS) before final classification of any
material that there is a question about as they may have stricter
requirements.

Now if you are speaking about the transport of Patient Specimens as
defined by IATA, exempt human or animal, which formalin fixed tissue
typically falls into then what is required is a leak-proof primary
receptacle, leak-proof secondary packaging and a rigid outer packaging
with size dimensions of no less than 100 mm x 100 mm.  

And my final disclaimer will be that you should always check with your
EHS department to make sure that you are following your
institutional/company policies.

Regards,

Jason 

Jason Burrill

Sr. Manager, Histology and Laboratory Safety

Research Animal Diagnostic Services

Charles River

251 Ballardvale St

Wilmington, MA 01887

Office: 781-222-6152

Fax: 978-988-8793

jason.burrill <@t> crl.com 

www.criver.com <http://www.criver.com/> 

Accelerating Drug Development. Exactly.

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------------------------------

Message: 2

Date: Fri, 18 Jul 2008 13:34:46 -0400

From: "Patricia Karlisch" <Pkarlisch <@t> hmc.psu.edu>

Subject: [Histonet] Formalin in plastic bags

To: <Histonet <@t> lists.utsouthwestern.edu>

Message-ID: <48809BF6.07F7.008C.0 <@t> hmc.psu.edu>

Content-Type: text/plain; charset=US-ASCII

Richard,

   There are standards for sending specimens that are in a hazardous
solution, like Formalin.  Plastic bags are secondary containers and
should not be used to transport fluid. The specimens should be in a
secure  plastic container that seals tightly and will not puncture
causing injury to others.   

   Pat Karlisch

Pat Karlisch

Supervisor, Histology, Pathology and Laboratory Medicine

Penn State Milton S. Hershey Medical Center

Mail Code H179

Hershey, PA 17033

Phone (717) 531-6072

Fax: (717) 531- 7741

email: pkarlisch <@t> psu.edu 

*****E-Mail Confidentiality Notice*****

This message (including any attachments) contains information intended
for a specific individual(s) and purpose that may be privileged,
confidential or otherwise protected from disclosure pursuant to
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to the sender indicating this error and delete the transmission from
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------------------------------

Message: 2
Date: Sat, 19 Jul 2008 19:56:28 -0700 (PDT)
From: shazana hilda <hilda_1075 <@t> yahoo.com>
Subject: [Histonet] troubleshoot of positive controls for PKCalpha and
eNOSantibody
To: histonet <@t> lists.utsouthwestern.edu 
Message-ID: <939284.12676.qm <@t> web38806.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Dear Histonetters,

I'm having difficulties to obtain positive staining of positive control for PKCalpha (H-7:sc-8393) and NOS3(c-20:sc-654) antibody. Both antibody were purchased from SantaCruz Biotechnology Inc.and used for IHC.Below are the methods that i used:

1)PKCalpha
    -incubation time: 1hour,room temperature (24-25 celcius)
    -Antibody dilution: 1:50
    - Target Antigen Retrieval Buffer: Citrate Buffer, pH6
    -Device: microwave (3x at 600W for 5min)
    -Detection method: polymeric method by LabVision
2) NOS3 <@t> eNOS
    -incubation time: 1hour,room temperature (24-25 celcius)
    -Antibody dilution: 1:50
    - Target Antigen Retrieval Buffer: Citrate Buffer, pH6
    -Device: microwave (3x at 600W for 5min)
    -Detection method: polymeric method by LabVision

I've used normal rat kidney as suggested by few journals for positive controls of both antibody. however my positive controls produced negative staining.

Your suggestion and quick response are highly appreciated

Shazana Hilda Shamsuddin
MSc.of Molecular Pathology,
Dept. of Pathology,
School of Medical Sciences,
Universiti Sains Malaysia,
16150 Kubang Kerian,
Kelantan, Malaysia.
Email: hilda_1075 <@t> yahoo.com 

------------------------------

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End of Histonet Digest, Vol 56, Issue 23
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