[Histonet] Restaining IHC slides

[Rene J Buesa]

If the new antibody you are going to use reacts in a different place as NSE does, it would be better to just leave the NSE as is, do the new retrieval and new antibody.
If the new Ab is going to react in the same place as NSE did, you are in trouble because any "destainig" always is going to be, in the best case, just partial (incomplete).
René J.

--- On Wed, 7/16/08, Christine I. Braaten <CBraaten <@t> Cheshire-Med.COM> wrote:

From: Christine I. Braaten <CBraaten <@t> Cheshire-Med.COM>
Subject: [Histonet] Restaining IHC slides
To: histonet <@t> lists.utsouthwestern.edu
Date: Wednesday, July 16, 2008, 9:11 AM

Good morning histonet subscribers! I have a question about restaining
previously stained slides for IHC. I know I have seen this before but
cannot access the archives. If I have already stained a slide for NSE
can I destain it and stain it with a different antibody? They have
different retrievals so should I retrieve again? Thanks in advance for
your replies. Christine


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