[Histonet] Saturated Fat Staining

[Griffin-Reyes, Michelle A]

I am having some troubles finding a way to be able to stain saturated
lipids in FFPE mouse tissues. Osmium tetroxide and oil red O stain well
but they are only showing unsaturated lipids. My lab is looking to see
if there is a difference in lipid content of treated mice vs. control
mice. So far, we have seen very little variability in the amount of
unsaturated lipids during quantification which leads us to believe there
may be a difference in saturated fats (based off of human results that
we have our mouse model based off of). Does anyone have any suggestions?
Thanks so much for the help!

Michelle A. Griffin-Reyes
Comparative Pathology Laboratory
University of Iowa: College of Medicine



Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged.  If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited.  Please reply to the sender that you have received the message in error, then delete it.  Thank you.