[Histonet] Re:H&E quality check

[R C]

It has been my experience that mistakes are contained and corrected in the
histology lab, and forward only quality work to the Pathologists. This
allows time to be categorized as beneficial.

Ruben Carter

On Tue, Jul 8, 2008 at 10:01 AM, <histonet-request <@t> lists.utsouthwestern.edu>
wrote:

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> Today's Topics:
>
>   1. Re: Antibody advice. (Andrea Hooper)
>   2. AW: [Histonet] H&E quality check (Gudrun Lang)
>   3. antibodies (koellingr <@t> comcast.net)
>   4. gutterguard (Paula Pierce)
>   5. Re: AW: [Histonet] H&E quality check (Jon Google)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Tue, 08 Jul 2008 10:56:08 -0400
> From: "Andrea Hooper" <anh2006 <@t> med.cornell.edu>
> Subject: Re: [Histonet] Antibody advice.
> To: Histonet <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <p06200700c4993018e018@[140.251.51.13]>
> Content-Type: text/plain; charset=us-ascii; format=flowed
>
> F4/80 from Serotec works very well in FFPE sections, haven't tried in
> frozen
> CD4 from BD Pharmingen is beautiful in frozens, I understand it
> doesn't work in FFPE
> CD3 from BD Pharmingen is beautiful in frozens, I understand it DOES
> work in FFPE
> Ly-6/Gr-1 from BD Pharmingen is beautiful in frozens, I haven't tested in
> FFPE
>
> >            Co-worker wants to look at macrophage using F4/80, neutrophils
> >  using Ly-6 and T-cells with CD3 and CD4. Any suggestions for a reliable
> >  source of the antibodies?
> >
> >  Ian.
> >
> >
> >
> >
> >
> >  Dr. Ian Montgomery,
> >
> >  Histotechnology,
> >
> >  I.B.L.S. Support Unit,
> >
> >  Thomson Building,
> >
> >  University of Glasgow,
> >
> >  Glasgow,
>
> --
>
>
>
> ------------------------------
>
> Message: 2
> Date: Tue, 8 Jul 2008 17:01:54 +0200
> From: "Gudrun Lang" <gu.lang <@t> gmx.at>
> Subject: AW: [Histonet] H&E quality check
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <0402798811164941817A9404B958C1CB <@t> dielangs.at>
> Content-Type: text/plain;       charset="iso-8859-1"
>
> I am with Renč. The slides rejected are for our lab 0,001 slides of all cut
> slides per year. The additional time for those few slides is acceptable.
> The
> only cause to reject a slide is the impossibility to read it correctly, or
> if the orientation of the tissue is false.
> By the way. I like to check the HE stain without the microscope. If it is
> too blue or too red, you see it from a distance. ;)
>
> Gudrun Lang
>
> Biomed. Analytikerin
> Histolabor
> Akh Linz
> Krankenhausstr. 9
> 4020 Linz
> +43(0)732/7806-6754
>
> -----Ursprüngliche Nachricht-----
> Von: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Rene J
> Buesa
> Gesendet: Dienstag, 08. Juli 2008 13:47
> An: stephanie.d.rivera <@t> gsk.com; Angela Bitting
> Cc: histonet; histonet-bounces <@t> lists.utsouthwestern.edu
> Betreff: Re: [Histonet] H&E quality check
>
> If you get to a percentage, then you will waste your whole day doing that.
> What I used to do is to let the pathologists reject the slides, then I
> reviewed them, found out who cut them, discuss the issue, fill the QA and
> retrain the HT who did the bad slide.Any other approach will be a total
> loss
> of precious time.Don't even think if giving a form to the pathologists to
> fill out the problem they found, they are not going to do it, and if they
> do, they will be wasting their time. That is the supervisor's task, the PT
> task is to reject, the supervisor's task is to prevent the problem from
> occurring again.
> René J.
>
> --- On Mon, 7/7/08, Angela Bitting <akbitting <@t> geisinger.edu> wrote:
>
>
>
>
>
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>
>
>
> ------------------------------
>
> Message: 3
> Date: Tue, 08 Jul 2008 15:22:01 +0000
> From: koellingr <@t> comcast.net
> Subject: [Histonet] antibodies
> To: ian.montgomery <@t> bio.gla.ac.uk>, <histonet <@t> lists.utsouthwestern.edu
> Message-ID:
>        <
> 070820081522.11460.48738619000161FF00002CC422007504389D09020704040A0105 <@t> comcast.net
> >
>
>
> Ian, just curious,
>
> Were you asking about murine or human F4-80, Ly-6, 3 and 4?  Seeing F4-80
> (which is a mouse molecule target although there are human equivalents for
> macs) and Ly-6 which generally you think of in mouse terms (although the
> human equivalent family of molecules has been described), I was wondering if
> this is for human or mouse tissues?
>
> Ray Koelling
> PhenoPath Labs
> Seattle, WA
>
>
>
> ------------------------------
>
> Message: 4
> Date: Tue, 8 Jul 2008 08:31:12 -0700 (PDT)
> From: Paula Pierce <contact <@t> excaliburpathology.com>
> Subject: [Histonet] gutterguard
> To: Histonet <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <869375.6562.qm <@t> web50108.mail.re2.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
>
>  Hi Margaret,
> I found mine at Ace Hardware. I also use it to make baskets of all sizes
> for processing. Paula Pierce, HTL(ASCP)HT
> Excalibur Pathology, Inc.
> 631 N. Broadway Ave.
> Moore, OK 73160
> 405-759-3953
> contact <@t> excaliburpathology.com
> www.excaliburpathology.com
>
> ------------------------------
>
> Message: 5
> Date: Tue, 8 Jul 2008 09:32:01 -0700 (PDT)
> From: Jon Google <ht.ascp <@t> yahoo.com>
> Subject: Re: AW: [Histonet] H&E quality check
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <701357.15100.qm <@t> web59904.mail.ac4.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> If it is all about time, the pathologist's time is more important.  If it
> takes them longer to read the slide because of cutting artifact, then that
> is more of a waste.  The lab should take the time to turn out a high quality
> slide, and ensure the ongoing quality throughout the day. Quicker screening
> and easier screening by the pathologists will lead to better TAT and less
> misdiagnosis.
>
> --- On Tue, 7/8/08, Gudrun Lang <gu.lang <@t> gmx.at> wrote:
>
> From: Gudrun Lang <gu.lang <@t> gmx.at>
> Subject: AW: [Histonet] H&E quality check
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Tuesday, July 8, 2008, 3:01 PM
>
> I am with Renč. The slides rejected are for our lab 0,001 slides of all cut
> slides per year. The additional time for those few slides is acceptable.
> The
> only cause to reject a slide is the impossibility to read it correctly, or
> if the orientation of the tissue is false.
> By the way. I like to check the HE stain without the microscope. If it is
> too blue or too red, you see it from a distance. ;)
>
> Gudrun Lang
>
> Biomed. Analytikerin
> Histolabor
> Akh Linz
> Krankenhausstr. 9
> 4020 Linz
> +43(0)732/7806-6754
>
> -----Ursprüngliche Nachricht-----
> Von: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Rene J
> Buesa
> Gesendet: Dienstag, 08. Juli 2008 13:47
> An: stephanie.d.rivera <@t> gsk.com; Angela Bitting
> Cc: histonet; histonet-bounces <@t> lists.utsouthwestern.edu
> Betreff: Re: [Histonet] H&E quality check
>
> If you get to a percentage, then you will waste your whole day doing that.
> What I used to do is to let the pathologists reject the slides, then I
> reviewed them, found out who cut them, discuss the issue, fill the QA and
> retrain the HT who did the bad slide.Any other approach will be a total
> loss
> of precious time.Don't even think if giving a form to the pathologists to
> fill out the problem they found, they are not going to do it, and if they
> do, they will be wasting their time. That is the supervisor's task, the PT
> task is to reject, the supervisor's task is to prevent the problem from
> occurring again.
> René J.
>
> --- On Mon, 7/7/08, Angela Bitting <akbitting <@t> geisinger.edu> wrote:
>
>
>
>
>
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> End of Histonet Digest, Vol 56, Issue 11
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