[Histonet] Re: Histonet Digest, Vol 56, Issue 9
richard shook
rshooki_99 <@t> yahoo.com
Tue Jul 8 07:55:22 CDT 2008
VWR has the metal slide trays #48469-004
----- Original Message ----
From: "histonet-request <@t> lists.utsouthwestern.edu" <histonet-request <@t> lists.utsouthwestern.edu>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Tuesday, July 8, 2008 8:06:49 AM
Subject: Histonet Digest, Vol 56, Issue 9
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Today's Topics:
1. Metal Slide Trays (Metzger, Kenneth)
2. RE: H&E quality check (Christine Bark)
3. RE: Metal Slide Trays (Bartlett, Jeanine (CDC/CCID/NCZVED))
4. Vector Labs Nova HRP--Red Substrate: Not Really EtOH
compatible? (JR R)
5. Help needed for brain processing (Shirley Powell)
6. Antibody for the detection of human cells needed (Alexandra Meinl)
7. tissue processor (Atoska Gentry)
8. Re: Gomeri Iron Stain (Maxim_71 <@t> mail.ru)
9. Leitz 1512 rotary microtome (sharon.osborn <@t> comcast.net)
10. RE: Antibody for the detection of human cells needed
(Liz Chlipala)
11. Re: H&E quality check (Robert Richmond)
12. Re: H&E quality check (Angela Bitting)
13. RE: Re: H&E quality check (Michael Mihalik)
14. HT position available (LINDA MARGRAF)
15. RE: H&E quality check (Weems, Joyce)
16. RE: Re: H&E quality check. . (Henry, Charlene)
17. RE: Re: H&E quality check. . (Michael Mihalik)
18. RE: Gomeri Iron Stain (Tony Henwood)
19. Re: H&E quality check. (Dave & Lee Mayhew)
20. RE: Re: H&E quality check. (Michael Mihalik)
21. Re: Re: H&E quality check. (Dave & Lee Mayhew)
22. Re: Re: H&E quality check. . (Anne van Binsbergen)
23. Re: H&E quality check (Rene J Buesa)
24. RE: Re: H&E quality check. . (Cheri Miller)
----------------------------------------------------------------------
Message: 1
Date: Mon, 7 Jul 2008 11:33:15 -0600
From: "Metzger, Kenneth" <kenneth.metzger <@t> aruplab.com>
Subject: [Histonet] Metal Slide Trays
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<BB644A4EB18113488C431FC9D5E4584A01D06703 <@t> postoffice01.aruplab.net>
Content-Type: text/plain; charset="iso-8859-1"
Does anyone know where I can order the 20 slot metal slide trays? Thanks,
ken
Ken Metzger HTL(ASCP)
Histology Supervisor
ARUP Laboratories
500 Chipeta way
Salt Lake City, UT 84108
801.583.2787 ext 3101
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------------------------------
Message: 2
Date: Mon, 7 Jul 2008 10:37:27 -0700
From: "Christine Bark" <CBark <@t> memorialcare.org>
Subject: [Histonet] RE: H&E quality check
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<FBF4E4324E7BB943B7413B2D84C5144E02B83092 <@t> mhsexchbe01.memnet.org>
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We check several different slides from our first rack of the day for H&E
and cutting quality.
Christine Bark HT(ASCP)
Senior Histotech, Pathology
Saddleback Memorial Medical Center
949-452-3548
cbark <@t> memorialcare.org
-----Original Message-----
From: "Angela Bitting" <akbitting <@t> geisinger.edu>
Subject: [Histonet] H&E quality check
Just curious as to how other hospital labs quality check their H&E
slides? Do most review a percentage microscopically?
______________________________________________________________________________
Confidentiality Notice: This e-mail message, including any attachments, is for
the sole use of the intended recipient(s) and may contain confidential and
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------------------------------
Message: 3
Date: Mon, 7 Jul 2008 13:40:13 -0400
From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" <jqb7 <@t> cdc.gov>
Subject: RE: [Histonet] Metal Slide Trays
To: "Metzger, Kenneth" <kenneth.metzger <@t> aruplab.com>,
histonet <@t> lists.utsouthwestern.edu
Message-ID:
<1CE1847DFEA0A647B1CCDE4108EA60A7F23C7E <@t> LTA3VS011.ees.hhs.gov>
Content-Type: text/plain; charset=us-ascii
I think this is what you are looking for.
http://www.daigger.com/catalog/product?deptId=&prodId=16050
Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartlett <@t> cdc.hhs.gov
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Metzger,
Kenneth
Sent: Monday, July 07, 2008 1:33 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Metal Slide Trays
Does anyone know where I can order the 20 slot metal slide trays?
Thanks,
ken
Ken Metzger HTL(ASCP)
Histology Supervisor
ARUP Laboratories
500 Chipeta way
Salt Lake City, UT 84108
801.583.2787 ext 3101
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the intended recipient, please promptly delete this e-mail and notify
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------------------------------
Message: 4
Date: Mon, 7 Jul 2008 10:54:49 -0700
From: JR R <rosenfeldtek <@t> hotmail.com>
Subject: [Histonet] Vector Labs Nova HRP--Red Substrate: Not Really
EtOH compatible?
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BAY135-W50A07D2BE98D70FD6C5210DB940 <@t> phx.gbl>
Content-Type: text/plain; charset="Windows-1252"
The Product information says:
"NovaRED™ substrate may be
dehydrated in ethanol and cleared in organic solvents such as xylene
and permanently mounted in a non-aqueous mounting medium such as
VectaMount"
But I have done two stains now where I initially had intense, specific staining at first--But after my slides come out of the EtOH/Xylene for coverslipping, the staining has vanished.
This is getting to be expensive!
Has anyone else ever had this problem with Nova Red? Any ideas?
Jerry Ricks
Research Scientist
University of Washington
Department of Pathology
_________________________________________________________________
Need to know now? Get instant answers with Windows Live Messenger.
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------------------------------
Message: 5
Date: Mon, 07 Jul 2008 14:07:27 -0400
From: Shirley Powell <POWELL_SA <@t> Mercer.edu>
Subject: [Histonet] Help needed for brain processing
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <01MWVKZ3Y6Y200ANAP <@t> Macon2.Mercer.edu>
Content-Type: text/plain; charset=us-ascii
A friend of mine, who has been banned from histonet by a micro-managing
employer, needs advice from those who use the microwave to expedite fixation
of whole or partial brains for routine processing, or any other rapid means
of getting complete fixation on brain. Please send all responses to me
personally at this address and I will forward the information to them.
Thanks in advance for your help.
Shirley Powell
------------------------------
Message: 6
Date: Mon, 7 Jul 2008 20:24:22 +0200
From: "Alexandra Meinl" <alexandra.meinl <@t> gmail.com>
Subject: [Histonet] Antibody for the detection of human cells needed
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<b7eff7a0807071124r2c7d0fdfu5a7b56a35f37f3fc <@t> mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
Hi all,
I'm searching for an antibody for the detection of human cells in mouse
tissue.
And: we don't know what type of cells we're trying to find.
I thought about an ab like a-hu-nuclear membrane or GM 130.
Does anybody this? What antibody would you recommend?
Any suggestions greatly appreaciated!
Alexandra
------------------------------
Message: 7
Date: Mon, 07 Jul 2008 13:35:13 -0500
From: Atoska Gentry <gentras <@t> vetmed.auburn.edu>
Subject: [Histonet] tissue processor
To: Histonet <histonet <@t> pathology.swmed.edu>
Message-ID: <487261E1.3020101 <@t> vetmed.auburn.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
hello, please will someone who has info on a modern tissue processor
comparable to an obsolete 25 yr.Autotechnicon Ultra II Tissue
Processor please contact me with specifications, product info & pricing
ASAP? Thanks, Atoska
------------------------------
Message: 8
Date: Mon, 7 Jul 2008 22:30:20 +0400
From: Maxim_71 <@t> mail.ru
Subject: Re: [Histonet] Gomeri Iron Stain
To: histology <@t> gradymem.org
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <228377341.20080707223020 <@t> mail.ru>
Content-Type: text/plain; charset=us-ascii
Angie:
We never used MWO modification for Gomori Iron Stain.
Iron is beautifully can demonsrtate at RT for 10 mins.
Vapours of cyanid is very toxic for personnel.
Maxim Peshkov
Russia,
Taganrog.
------------------------------
Message: 9
Date: Mon, 07 Jul 2008 19:11:41 +0000
From: sharon.osborn <@t> comcast.net
Subject: [Histonet] Leitz 1512 rotary microtome
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<070720081911.18025.48726A6D0006F323000046692215555884029D010D9C01D202019D0E089C <@t> comcast.net>
Histonetters,
I have a copy of the instructions manual for above referenced microtome should anyone still have this workhorse and need the instructions. Please send me your contact information and I am happy to send out. If there is more than one person requesting it, I can make copies.
sharon osborn
Lab Vision
Thermo Fisher
Fremont, CA
------------------------------
Message: 10
Date: Mon, 7 Jul 2008 13:20:33 -0600
From: "Liz Chlipala" <liz <@t> premierlab.com>
Subject: RE: [Histonet] Antibody for the detection of human cells
needed
To: "Alexandra Meinl" <alexandra.meinl <@t> gmail.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<EE33BE5C905A3046A7FF8F58A64C8E4B08E955 <@t> server.PremierLab.local>
Content-Type: text/plain; charset="us-ascii"
Alexandra
We have tried the human anti-nuclear antibody from chemicon but we did
not have much success with it. We have used a human mitochondrial
antibody from Novus with good success but in rat tissues rather than
mouse, it's generated in mouse but as long as you use the correct
controls it should work. We have also ran In-situ with a repeat
sequence for human DNA and that worked nicely between mouse and also rat
and human cells. We had our probe made by GeneDetect.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 682-3949
fax (303) 682-9060
liz <@t> premierlab.com
www.premierlab.com
Ship to Address:
Premier Laboratory, LLC
1567 Skyway Drive
Unit E
Longmont, CO 80504
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Alexandra Meinl
Sent: Monday, July 07, 2008 12:24 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Antibody for the detection of human cells needed
Hi all,
I'm searching for an antibody for the detection of human cells in mouse
tissue.
And: we don't know what type of cells we're trying to find.
I thought about an ab like a-hu-nuclear membrane or GM 130.
Does anybody this? What antibody would you recommend?
Any suggestions greatly appreaciated!
Alexandra
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 11
Date: Mon, 7 Jul 2008 15:34:27 -0400
From: "Robert Richmond" <RSRICHMOND <@t> aol.com>
Subject: [Histonet] Re: H&E quality check
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<abea52a60807071234p53eeebbcm16daca58b1d7b4a3 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Well, I've worked in roughly 60 hospital and private lab pathology
services in my locum tenens career, and I've never seen a functioning
QA (or whatever they call it this year) program for H & E (or any
other stain) in a pathology lab. An occasional lab has the pathologist
fill out QA sheets telling them that the slides are wonderful -
invariably in such labs the slides are horrible. In most labs nobody
but the pathologist ever looks at a slide under a microscope.
A meaningful QA program would have a pathologist and a senior
histotechnologist review some of the day's production, during the
working day. I've always been ridiculed for suggesting it.
Bob Richmond
Samurai Pathologist
Knoxville TN
------------------------------
Message: 12
Date: Mon, 07 Jul 2008 15:47:21 -0400
From: "Angela Bitting" <akbitting <@t> geisinger.edu>
Subject: Re: [Histonet] H&E quality check
To: <stephanie.d.rivera <@t> gsk.com>
Cc: histonet <histonet <@t> lists.utsouthwestern.edu>,
histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID: <48723A89.2B7F.00C9.0 <@t> geisinger.edu>
Content-Type: text/plain; charset=US-ASCII
Thanks for all of the replies. I guess I need to rephrase my question. I'm wondering how many slides are reviewed daily under the scope for cutting artifacts, incomplete margins, etc. Where I worked before, we checked 10% of the slides under the scope before sending them on to the Pathologists. Do other labs do this?
>>> <stephanie.d.rivera <@t> gsk.com> 7/7/2008 9:49 AM >>>
Everywhere I have worked there was a liver control slide run daily for
Q.C. The techs checked the staining quality before beginning staining for
the day. The slide was dated and documented in the log book.
Stephanie D. Rivera
Safety Assessment Department
GlaxoSmithKline
709 Swedeland RD
King of Prussia, PA 19406
phone: 610-270-7340
fax: 610-270-7202
"Angela Bitting" <akbitting <@t> geisinger.edu>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
07-Jul-2008 07:59
To
"histonet" <histonet <@t> lists.utsouthwestern.edu>
cc
Subject
[Histonet] H&E quality check
Just curious as to how other hospital labs quality check their H&E slides?
Do most review a percentage microscopically?
IMPORTANT WARNING: The information in this message (and the documents
attached to it, if any) is confidential and may be legally privileged. It
is intended solely for the addressee. Access to this message by anyone
else is unauthorized. If you are not the intended recipient, any
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------------------------------
Message: 13
Date: Mon, 7 Jul 2008 15:50:45 -0400
From: "Michael Mihalik" <mike <@t> pathview.com>
Subject: RE: [Histonet] Re: H&E quality check
To: "'Robert Richmond'" <RSRICHMOND <@t> aol.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <A43AE1C97DB34887B964952B908C04AD <@t> MDMM1330>
Content-Type: text/plain; charset="US-ASCII"
To tag along with this statement. Wouldn't it be nice if the pathologist
could comment on the 'quality' of the stain as they are reviewing the slide
in the first place?
...or am I missing something here?
Michael Mihalik
PathView Systems | office: 207.483.0968 | cell: 214.733.7688 | 800.798.3540
| fax: 270.423.0968
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Robert
Richmond
Sent: Monday, July 07, 2008 3:34 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: H&E quality check
Well, I've worked in roughly 60 hospital and private lab pathology
services in my locum tenens career, and I've never seen a functioning
QA (or whatever they call it this year) program for H & E (or any
other stain) in a pathology lab. An occasional lab has the pathologist
fill out QA sheets telling them that the slides are wonderful -
invariably in such labs the slides are horrible. In most labs nobody
but the pathologist ever looks at a slide under a microscope.
A meaningful QA program would have a pathologist and a senior
histotechnologist review some of the day's production, during the
working day. I've always been ridiculed for suggesting it.
Bob Richmond
Samurai Pathologist
Knoxville TN
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 14
Date: Mon, 07 Jul 2008 15:02:20 -0500
From: "LINDA MARGRAF" <LINDA.MARGRAF <@t> childrens.com>
Subject: [Histonet] HT position available
To: <histonet <@t> lists.utsouthwestern.edu>
Cc: BETHTEWS <@t> HOTMAIL.COM
Message-ID: <48722FFC020000DA0002B8BF <@t> CNET3.CHILDRENS.COM>
Content-Type: text/plain; charset="iso-8859-15"
Here's a message Beth asked me to post for her.
Please reply to her if you are interested.
Thanks
LM (histonet administrator)
Technical Applications specialist- laboratory
ESSENTIAL DUTIES AND RESPONSIBILITIES
Installation of instrumentation and conducting user training to
successfully integrate products within the customer laboratory.
Provide troubleshooting and minor repair to instrumentation.
Work with customers to resolve reagent/staining application issues.
Communicate with dispatch and area management on daily/weekly/monthly
activities (eg. Salesforce.com)
Maintaining tools and test equipment in accordance with Quality
procedures
Conduct in-service training for customers.
Customer follow-up
Submit administrative paperwork in a timely and accurate manner.
installation forms
service work orders
expense reports
Travel extensively within the region and throughout the country.
REQUIRED EDUCATION & EXPERIENCE
Associates degree (or equivalent) or higher in Applied Sciences or
accredited Medical/Histology Technology course. Minimum one year
general clinical laboratory experience or technical equivalent which may
include general histology, cytology, immunohistochemistry, in situ
hybridization, biology research or work with automated staining
instrumentation.
CERTIFICATES, LICENSES, REGISTRATIONS
Highly desirable:
HISTOLOGY TECHNICIAN
American Society of Clinical Pathologists Certification HT or HTL.
American Society of Clinical Pathologists Qualification in
Immunohistochemistry (QIHC).
KNOWLEDGE, SKILLS & ABILITIES
Must have the ability to read, analyze, and interpret technical
procedures or governmental regulations (CAP, CLIA). Ability to write
reports. Ability to effectively present information and respond to
questions from customers. Ability to calculate antibody dilutions.
Ability to interpret a variety of instructions furnished in written,
verbal, diagram, or schedule form.
WORK ENVIRONMENT
The work environment characteristics described here are representative
of those an employee encounters while performing the essential functions
of this job traveling to and working within a Clinical Pathology Lab,
Research lab, and animal diagnostic lab. Reasonable accommodations may
be made to enable individuals with disabilities to perform the essential
functions.
OTHER QUALIFICATIONS
Ability to travel.
Valid driver*s license. with a clean driving record.
COMPENSATION PACKAGE
POSITION OFFERS A COMPETITVE BASE SALARY AND A CAR PLUS ALL TRAVEL
EXPENSES AND BENEFITS.
CONTACT BETH TEWS RECRUITING
BETHTEWS <@t> HOTMAIL.COM
623-742-7227
OPENINGS CURRENTLY NEED TO BE FILLED BY 715/08 IN PHOENIX, NYC/NJ,ST
LOUIS, BOSTON MORE TO COME SO PLEASE APPLY
</pre> <span style="color: rgb(0, 160, 0); font-weight: bold;">Please consider the environment before printing this e-mail</span><br />
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</html>
------------------------------
Message: 15
Date: Mon, 7 Jul 2008 16:14:06 -0400
From: "Weems, Joyce" <JWeems <@t> sjha.org>
Subject: RE: [Histonet] H&E quality check
To: "Angela Bitting" <akbitting <@t> geisinger.edu>,
<stephanie.d.rivera <@t> gsk.com>
Cc: histonet <histonet <@t> lists.utsouthwestern.edu>,
histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
<982A0A9461F9BF438C7B19A6E425A383373163 <@t> ITSSSXM01V6.one.ads.che.org>
Content-Type: text/plain; charset="us-ascii"
We do 1 or 2 per full tray - recording wrinkles, folds, poor staining,
etc. to get an approximate 10% reviewed.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Angela
Bitting
Sent: Monday, July 07, 2008 3:47 PM
To: stephanie.d.rivera <@t> gsk.com
Cc: histonet; histonet-bounces <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] H&E quality check
Thanks for all of the replies. I guess I need to rephrase my question.
I'm wondering how many slides are reviewed daily under the scope for
cutting artifacts, incomplete margins, etc. Where I worked before, we
checked 10% of the slides under the scope before sending them on to the
Pathologists. Do other labs do this?
>>> <stephanie.d.rivera <@t> gsk.com> 7/7/2008 9:49 AM >>>
Everywhere I have worked there was a liver control slide run daily for
Q.C. The techs checked the staining quality before beginning staining
for the day. The slide was dated and documented in the log book.
Stephanie D. Rivera
Safety Assessment Department
GlaxoSmithKline
709 Swedeland RD
King of Prussia, PA 19406
phone: 610-270-7340
fax: 610-270-7202
"Angela Bitting" <akbitting <@t> geisinger.edu> Sent by:
histonet-bounces <@t> lists.utsouthwestern.edu
07-Jul-2008 07:59
To
"histonet" <histonet <@t> lists.utsouthwestern.edu>
cc
Subject
[Histonet] H&E quality check
Just curious as to how other hospital labs quality check their H&E
slides?
Do most review a percentage microscopically?
IMPORTANT WARNING: The information in this message (and the documents
attached to it, if any) is confidential and may be legally privileged.
It is intended solely for the addressee. Access to this message by
anyone else is unauthorized. If you are not the intended recipient, any
disclosure, copying, distribution or any action taken, or omitted to be
taken, in reliance on it is prohibited and may be unlawful. If you have
received this message in error, please delete all electronic copies of
this message (and the documents attached to it, if any), destroy any
hard copies you may have created and notify me immediately by replying
to this email. Thank you.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Confidentiality Notice:
This email, including any attachments is the
property of Catholic Health East and is intended
for the sole use of the intended recipient(s).
It may contain information that is privileged and
confidential. Any unauthorized review, use,
disclosure, or distribution is prohibited. If you are
not the intended recipient, please reply to the
sender that you have received the message in
error, then delete this message.
------------------------------
Message: 16
Date: Mon, 7 Jul 2008 15:14:08 -0500
From: "Henry, Charlene" <Charlene.Henry <@t> STJUDE.ORG>
Subject: RE: [Histonet] Re: H&E quality check. .
To: 'Michael Mihalik' <mike <@t> pathview.com>, 'Robert Richmond'
<RSRICHMOND <@t> aol.com>, "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<03E1F5968F60C5448635D49D38B283ED01460FD918 <@t> SJMEMXMBS11.stjude.sjcrh.local>
Content-Type: text/plain; charset="us-ascii"
Michael,
Actually it is now a CAP requirement.
ANP.11713 Is there documented evidence of daily review of the technical quality of histologic preparations by the pathologist.
This is the way we address this question.
Each morning while we are cutting surgical and bone marrow biopsies, we randomly select 2-3 blocks and cut a QC H&E slide in addition to the routine cuts. They are stained along the routine cases and these are given to our Director of AP for review. She then documents the quality of fixation, processing, cutting and staining on a log. While she is on vacation one of our other pathologists review the slides. When the log sheet is full, it is returned to me and filed with our QC records.
Charlene Henry HT (ASCP), QIHC
Anatomic Pathology Section Head
Department of Pathology
St. Jude Children's Research Hospital
901-495-3191
fax 901-495-3100
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Michael Mihalik
Sent: Monday, July 07, 2008 2:51 PM
To: 'Robert Richmond'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: H&E quality check. .
To tag along with this statement. Wouldn't it be nice if the pathologist could comment on the 'quality' of the stain as they are reviewing the slide in the first place?
...or am I missing something here?
Michael Mihalik
PathView Systems | office: 207.483.0968 | cell: 214.733.7688 | 800.798.3540
| fax: 270.423.0968
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Robert Richmond
Sent: Monday, July 07, 2008 3:34 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: H&E quality check
Well, I've worked in roughly 60 hospital and private lab pathology services in my locum tenens career, and I've never seen a functioning QA (or whatever they call it this year) program for H & E (or any other stain) in a pathology lab. An occasional lab has the pathologist fill out QA sheets telling them that the slides are wonderful - invariably in such labs the slides are horrible. In most labs nobody but the pathologist ever looks at a slide under a microscope.
A meaningful QA program would have a pathologist and a senior histotechnologist review some of the day's production, during the working day. I've always been ridiculed for suggesting it.
Bob Richmond
Samurai Pathologist
Knoxville TN
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------------------------------
Message: 17
Date: Mon, 7 Jul 2008 16:47:31 -0400
From: "Michael Mihalik" <mike <@t> pathview.com>
Subject: RE: [Histonet] Re: H&E quality check. .
To: "'Henry, Charlene'" <Charlene.Henry <@t> STJUDE.ORG>, "'Robert
Richmond'" <RSRICHMOND <@t> aol.com>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <E183D0CB02DB47A185539AEBCB7CFBA7 <@t> MDMM1330>
Content-Type: text/plain; charset="US-ASCII"
I appreciate the response. Let me ask this question another way. Is there
anybody who currently logs their histo QC in their computer system, not on
paper, not on some department created excel spreadsheet?
Would this not be desirable?
Michael Mihalik
PathView Systems | office: 207.483.0968 | cell: 214.733.7688 | 800.798.3540
| fax: 270.423.0968
-----Original Message-----
From: Henry, Charlene [mailto:Charlene.Henry <@t> STJUDE.ORG]
Sent: Monday, July 07, 2008 4:14 PM
To: 'Michael Mihalik'; 'Robert Richmond'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: H&E quality check. .
Michael,
Actually it is now a CAP requirement.
ANP.11713 Is there documented evidence of daily review of the technical
quality of histologic preparations by the pathologist.
This is the way we address this question.
Each morning while we are cutting surgical and bone marrow biopsies, we
randomly select 2-3 blocks and cut a QC H&E slide in addition to the routine
cuts. They are stained along the routine cases and these are given to our
Director of AP for review. She then documents the quality of fixation,
processing, cutting and staining on a log. While she is on vacation one of
our other pathologists review the slides. When the log sheet is full, it is
returned to me and filed with our QC records.
Charlene Henry HT (ASCP), QIHC
Anatomic Pathology Section Head
Department of Pathology
St. Jude Children's Research Hospital
901-495-3191
fax 901-495-3100
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Michael
Mihalik
Sent: Monday, July 07, 2008 2:51 PM
To: 'Robert Richmond'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: H&E quality check. .
To tag along with this statement. Wouldn't it be nice if the pathologist
could comment on the 'quality' of the stain as they are reviewing the slide
in the first place?
...or am I missing something here?
Michael Mihalik
PathView Systems | office: 207.483.0968 | cell: 214.733.7688 | 800.798.3540
| fax: 270.423.0968
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Robert
Richmond
Sent: Monday, July 07, 2008 3:34 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: H&E quality check
Well, I've worked in roughly 60 hospital and private lab pathology services
in my locum tenens career, and I've never seen a functioning QA (or whatever
they call it this year) program for H & E (or any other stain) in a
pathology lab. An occasional lab has the pathologist fill out QA sheets
telling them that the slides are wonderful - invariably in such labs the
slides are horrible. In most labs nobody but the pathologist ever looks at a
slide under a microscope.
A meaningful QA program would have a pathologist and a senior
histotechnologist review some of the day's production, during the working
day. I've always been ridiculed for suggesting it.
Bob Richmond
Samurai Pathologist
Knoxville TN
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 18
Date: Tue, 8 Jul 2008 09:19:30 +1000
From: "Tony Henwood" <AnthonyH <@t> chw.edu.au>
Subject: RE: [Histonet] Gomeri Iron Stain
To: "Histology Dept" <histology <@t> gradymem.org>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <B9EAF61856077F47BF9BE2F89AFC555202FAFF7F <@t> hedwig.nch.kids>
Content-Type: text/plain; charset="us-ascii"
The following works well though it is a Perl's stain. I can't remember
the Gomori Iron stain. The old cranium is shrinking as I get older!!
It was developed for frozen sections (eg differentiating melanin from
iron pigment) but works quite well for paraffin sections.
1 Placed ethanol fixed frozen sections or hydrated paraffin
section in an the Perl's reagent in a plastic coplin jar (20ml each of
2% Potassium ferrocyanide and 2% HCl).
Microwave for 20 sec at 650watts.
2. Leave sections in the hot solution for up to 3 minutes, remove
if the solution becomes cloudy.
3. Rinse in water.
4. Counterstain with eosin (as used for the frozen section H&E) or
a red nuclear stain (Nuclear Fast Red or Neutral red).
5. Rinse in water, DC&M.
Reference: Henwood (2002) "Microwave Perl's Stain for Urgent Frozen
Sections" Aust J Med Sc 23(2):68-69.
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Histology Dept
Sent: Tuesday, 8 July 2008 1:50 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Gomeri Iron Stain
We finally got a microwave for special stains. Can you use a microwave
method to do Gomeri Iron Stain? If so, would someone please share you
procedure with us?
Angie Barnett, HTL(ASCP)
Grady Memorial Hospital
Pathology Department
405/224-2258
histology <@t> gradymem.org
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------------------------------
Message: 19
Date: Mon, 7 Jul 2008 20:45:29 -0400
From: "Dave & Lee Mayhew" <lmayhew5 <@t> cogeco.ca>
Subject: [Histonet] Re: H&E quality check.
To: "Histonet" <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <008601c8e093$eb805690$e013c143 <@t> user4ab93baede>
Content-Type: text/plain; charset="iso-8859-1"
Hi Michael,
We use Meditech and create a daily QC specimen.
In the data fields for the QC specimen we record all the stains we did for that day, including H&E, and all the specimen numbers that were controlled by those QC slides. Any problems, concerns, repeats, etc. are recorded in the specimen comment field.
A daily report is printed out at the end of the day and filed in the QC binder.
Lee Mayhew MLT
St. Joseph's Hospital
Hamilton ON Canada
------------------------------
Message: 20
Date: Mon, 7 Jul 2008 21:16:13 -0400
From: "Michael Mihalik" <mike <@t> pathview.com>
Subject: RE: [Histonet] Re: H&E quality check.
To: "'Dave & Lee Mayhew'" <lmayhew5 <@t> cogeco.ca>, "'Histonet'"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <772604D2DC974271BCC24D75ED3FA5ED <@t> MDMM1330>
Content-Type: text/plain; charset="US-ASCII"
So you create a 'normal' case where the specimen is a qc case and then fill
in all the 'normal' fields with qc data.
Is that correct?
....or are there specialized qc data fields, like a prompt for stain
quality, block quality, etc.
I'm looking to see if any computer system does anything special for QC
purposes, relative to block, slides, stains, etc.
Michael Mihalik
PathView Systems | office: 207.483.0968 | cell: 214.733.7688 | 800.798.3540
| fax: 270.423.0968
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Dave & Lee
Mayhew
Sent: Monday, July 07, 2008 8:45 PM
To: Histonet
Subject: [Histonet] Re: H&E quality check.
Hi Michael,
We use Meditech and create a daily QC specimen.
In the data fields for the QC specimen we record all the stains we did for
that day, including H&E, and all the specimen numbers that were controlled
by those QC slides. Any problems, concerns, repeats, etc. are recorded in
the specimen comment field.
A daily report is printed out at the end of the day and filed in the QC
binder.
Lee Mayhew MLT
St. Joseph's Hospital
Hamilton ON Canada
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 21
Date: Mon, 7 Jul 2008 21:34:08 -0400
From: "Dave & Lee Mayhew" <lmayhew5 <@t> cogeco.ca>
Subject: Re: [Histonet] Re: H&E quality check.
To: "Michael Mihalik" <mike <@t> pathview.com>, "'Histonet'"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <00a501c8e09a$b7876bb0$e013c143 <@t> user4ab93baede>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
Michael
The case created is a special QC Specimen. Meditech allows you to create
any data fields that you want, they do not have to be the same data sections
as are found in patient specimens, so you could create a prompt for the
things you suggest if you wanted to. The workload that you enter into this
specimen is counted as quality control not patient workload.
I should say that we also have a daily sheet that the day's pathologist
fills out to document any of their concerns. We do not enter this into
Meditech, although we, or they, could.
Lee Mayhew MLT
> St. Joseph's Hospital
> Hamilton ON Canada
------------------------------
Message: 22
Date: Tue, 8 Jul 2008 08:52:10 +0400
From: "Anne van Binsbergen" <annigyg <@t> gmail.com>
Subject: Re: [Histonet] Re: H&E quality check. .
To: "Michael Mihalik" <mike <@t> pathview.com>
Cc: histonet <@t> lists.utsouthwestern.edu, "Henry, Charlene"
<Charlene.Henry <@t> stjude.org>, Robert Richmond <RSRICHMOND <@t> aol.com>
Message-ID:
<f8332fbe0807072152r5510962ei433d4dd461266a96 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
we have a tissue array (tonsil, kidney, skin, L/N, appendix) block which we
made ourselves by using a dermatology punch tool
identical slides are cut and kept in a box
each morning we stain one and it gets viewed by a senior tech - the path
does not have time for such matters!!
results logged, adjustments noted, signed off, logs filed, slides filed -
all ready for CAP to scrutinise
if we adjust the stain (or anything else) we run another QC slide and relog
the results
we are in the process of making an extended array for a whole bunch of
specials too
its a pain in the @$$ but my staff are used to the process now
ideally it should be done the 'samurai' way - but im just the tech - what do
i know!!! ;))
annieinarabia
2008/7/8 Michael Mihalik <mike <@t> pathview.com>:
> I appreciate the response. Let me ask this question another way. Is there
> anybody who currently logs their histo QC in their computer system, not on
> paper, not on some department created excel spreadsheet?
>
> Would this not be desirable?
>
>
> Michael Mihalik
> PathView Systems | office: 207.483.0968 | cell: 214.733.7688 | 800.798.3540
> | fax: 270.423.0968
>
>
>
>
> -----Original Message-----
> From: Henry, Charlene [mailto:Charlene.Henry <@t> STJUDE.ORG]
> Sent: Monday, July 07, 2008 4:14 PM
> To: 'Michael Mihalik'; 'Robert Richmond';
> histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Re: H&E quality check. .
>
> Michael,
> Actually it is now a CAP requirement.
>
> ANP.11713 Is there documented evidence of daily review of the technical
> quality of histologic preparations by the pathologist.
>
> This is the way we address this question.
> Each morning while we are cutting surgical and bone marrow biopsies, we
> randomly select 2-3 blocks and cut a QC H&E slide in addition to the
> routine
> cuts. They are stained along the routine cases and these are given to our
> Director of AP for review. She then documents the quality of fixation,
> processing, cutting and staining on a log. While she is on vacation one of
> our other pathologists review the slides. When the log sheet is full, it is
> returned to me and filed with our QC records.
>
>
> Charlene Henry HT (ASCP), QIHC
> Anatomic Pathology Section Head
> Department of Pathology
> St. Jude Children's Research Hospital
> 901-495-3191
> fax 901-495-3100
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Michael
> Mihalik
> Sent: Monday, July 07, 2008 2:51 PM
> To: 'Robert Richmond'; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Re: H&E quality check. .
>
> To tag along with this statement. Wouldn't it be nice if the pathologist
> could comment on the 'quality' of the stain as they are reviewing the slide
> in the first place?
>
> ...or am I missing something here?
>
> Michael Mihalik
> PathView Systems | office: 207.483.0968 | cell: 214.733.7688 | 800.798.3540
> | fax: 270.423.0968
>
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Robert
> Richmond
> Sent: Monday, July 07, 2008 3:34 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Re: H&E quality check
>
> Well, I've worked in roughly 60 hospital and private lab pathology services
> in my locum tenens career, and I've never seen a functioning QA (or
> whatever
> they call it this year) program for H & E (or any other stain) in a
> pathology lab. An occasional lab has the pathologist fill out QA sheets
> telling them that the slides are wonderful - invariably in such labs the
> slides are horrible. In most labs nobody but the pathologist ever looks at
> a
> slide under a microscope.
>
> A meaningful QA program would have a pathologist and a senior
> histotechnologist review some of the day's production, during the working
> day. I've always been ridiculed for suggesting it.
>
> Bob Richmond
> Samurai Pathologist
> Knoxville TN
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
--
Anne van Binsbergen (Hope)
Abu Dhabi
UAE
------------------------------
Message: 23
Date: Tue, 8 Jul 2008 04:46:40 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] H&E quality check
To: stephanie.d.rivera <@t> gsk.com, Angela Bitting
<akbitting <@t> geisinger.edu>
Cc: histonet <histonet <@t> lists.utsouthwestern.edu>,
histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID: <661700.14357.qm <@t> web65702.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
If you get to a percentage, then you will waste your whole day doing that.
What I used to do is to let the pathologists reject the slides, then I reviewed them, found out who cut them, discuss the issue, fill the QA and retrain the HT who did the bad slide.Any other approach will be a total loss of precious time.Don't even think if giving a form to the pathologists to fill out the problem they found, they are not going to do it, and if they do, they will be wasting their time. That is the supervisor's task, the PT task is to reject, the supervisor's task is to prevent the problem from occurring again.
René J.
--- On Mon, 7/7/08, Angela Bitting <akbitting <@t> geisinger.edu> wrote:
------------------------------
Message: 24
Date: Tue, 8 Jul 2008 07:05:20 -0500
From: "Cheri Miller" <cmiller <@t> physlab.com>
Subject: RE: [Histonet] Re: H&E quality check. .
To: "'Anne van Binsbergen'" <annigyg <@t> gmail.com>, "'Michael Mihalik'"
<mike <@t> pathview.com>
Cc: histonet <@t> lists.utsouthwestern.edu, "'Henry, Charlene'"
<Charlene.Henry <@t> stjude.org>, 'Robert Richmond' <RSRICHMOND <@t> aol.com>
Message-ID: <000901c8e0f2$e4e24280$3d02a8c0 <@t> plab.local>
Content-Type: text/plain; charset="us-ascii"
We check the slide quality daily by using a control slide (usually an
appendix or gallbladder) with the first rack of slides. I then check random
slides from each rack stained, we also have a QA sheet the paths fill out
daily. They grade us on grossing, specimen sampling, processing, embedding,
microtomy, staining and cover slipping. Most of them see this as a true
quality control indicator and let us know when we have an issue; a few just
fill it out without any real critiquing, which defeats the whole purpose.
Cheryl Miller HT (ASCP)
Histology Supervisor
Physicians Laboratory,P.C.
Omaha, Ne.
402 738 5052
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