[Histonet] PAS stain decolorizer?

[Mike Pence]

This brings up a point of interest. Would you not need to treat your
control tissue that you are going to now do a new stain in the same
manner to assure stain quality. Just wondering?

Mike

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Smith,
Allen
Sent: Thursday, July 03, 2008 10:53 AM
To: 'Carol Fields'
Cc: 'histonet <@t> lists.utsouthwestern.edu'
Subject: RE: [Histonet] PAS stain decolorizer?


My research assistant, Isaac Glickfield, was intrigued by the problem.
He found that overnight immersion in concentrated (i.e., 28%) ammonium
hydroxide removes the Schiff reagent and restores the original aldehydes
in the tissue.
                Allen A. Smith
              Barry University School of Podiatric Medicine
            Miami Shores, Florida

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Carol
Fields
Sent: Thursday, July 03, 2008 11:46 AM
To: Edie Lehman
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] PAS stain decolorizer?

Anatech or one of the companies make a powder that removes PAS from your
hands and clothes.  I mixed a small amount of the powder in a coplan jar
with dist H20 and put the slides in there for a few minutes and it took
the Schiff's out.  Then you wash them well and stain.

Carole Fields
Northside Hospital
Atlanta, GA

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Edie
Lehman
Sent: Thursday, July 03, 2008 11:30 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] PAS stain decolorizer?

We have a section that was incorrectly stained with PAS and no tissue
left to recut.  We need to do an H.Pylori stain on the slide.  Any
thoughts on how to destain the PAS?  Tech tried acid alcohol to no
avail.

Thanks

Edie Lehman MT(ASCP)
Anatomical Pathology Supervisor
Fairfield Medical Center


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