[Histonet] O.C.T. embedding vs. freezing of unembedded samples
gayle.callis <@t> bresnan.net
Fri Jan 25 09:31:24 CST 2008
The purpose of OCT is to support the tissue during cryotomy. I have done it
both ways and anything I have snap frozen without embedded will be embedded
in OCT prior to sectioning. Snap freezing of tissue can be done either
way, but we like OCT surrounding the tissue for sectioning, it also gives
the handle for those using brush technique versus antiroll device. If we do
not fill mouse lung and then support it with OCT, the sections and cryotomy
We also like the OCT around the tissues (blocks) for -80C storage for murine
animal model immunostaining purposes.
Gayle M. Callis
Bozeman MT 59715
----- Original Message -----
From: "Mareike Heimann" <mareike <@t> hi.is>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, January 25, 2008 4:39 AM
Subject: [Histonet] O.C.T. embedding vs. freezing of unembedded samples
I have been wondering about the advantages (or disadvantages) of embedding
tissue in O.C.T. before snap-freezing in liquid nitrogen when compared to
snap-freezing the "naked" tissue.
I do use O.C.T. before freezing, but when recently asked why, I found no
better answer than "it seems to be what most people do..."
I would be most grateful for any explanations!
(My tissue blocks are used for immunohistochemical stainings later on)
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