[Histonet] Ki67 in sheep

Liz Chlipala liz <@t> premierlab.com
Thu Jan 24 13:05:13 CST 2008


I have not tried this specific antibody in sheep, but we have used it in
mouse, rat, human, canine tissues, and possibly porcine, but I can't
remember off hand. Its from Santa Cruz	sc-15402	H-300	Rabbit
polyclonal, it might work in sheep.  I have a protocol if needed.


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
liz <@t> premierlab.com
Ship to Address:
Premier Laboratory, LLC
University of Colorado at Boulder
MCDB, Room A3B40
Boulder, CO 80309

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
AGrobe2555 <@t> aol.com
Sent: Thursday, January 24, 2008 12:01 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Ki67 in sheep

Hello all,
Can anyone recommend an antibody and staining protocol for Ki-67 that
can  be used in FFPE sheep tissues.  Normal tissue(s) to be used as a
control would also be helpful (we don't have access to anything but
normal  tissue).  We are currently trying to optimize the antibody we
have with  varying results....

Albert C.  Grobe, PhD
Tissue Engineering Lab
International Heart Institute of Montana  Foundation

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