[Histonet] Mucin containing cells too blue

[Tony Henwood]

Good,
I assume you would check the pH before you put it on the market.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




-----Original Message-----
From: Akemi Allison-Tacha [mailto:akemiat3377 <@t> yahoo.com] 
Sent: Wednesday, 23 January 2008 8:25 AM
To: Tony Henwood; Greg Dobbin; Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Mucin containing cells too blue


The pH of the Hematoxylin I developed for Biocare (CAT
Hematoxylin) a progressive hematoxylin, was between pH
2.26 to 2.3.  I would let it oxidize (ripened) for 3
months before releasing it for sale.  It would not
over-stain mucin, especially in the GI tract.  


--- Tony Henwood <AnthonyH <@t> chw.edu.au> wrote:

> Greg,
> 
> Don't assume that "fresh hematoxylin (and thererfore
> correct pH) would
> rectify the problem". Incorrect pH of the
> Haematoxylin is the usual
> problem. A feature of a haematoxylin solution that
> has a high pH is the
> staining of mucin, especially in the alimentary
> tract. Decreasing the pH
> of the staining solution to about 2.5 will decrease
> this mucin staining.
> 
> Regards
> 
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys,
> CT(ASC)
> Laboratory Manager & Senior Scientist
> The Children's Hospital at Westmead,
> Locked Bag 4001, Westmead, 2145, AUSTRALIA.
> Tel: 612 9845 3306
> Fax: 612 9845 3318
> 
> 
> 
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]
> On Behalf Of Greg
> Dobbin
> Sent: Wednesday, 23 January 2008 7:24 AM
> To: Histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Mucin containing cells too blue
> 
> 
> Hi Folks,
> I have been using the Surgipath Selectech reagents
> (H&E) for almost 3
> months now and I have not had a single complaint
> about staining...until
> now.  Suddenly the mucin containing cells are too
> blue.  My first
> thought was to make fresh Define (the Surgipath differentiator).  This
> did not fix the problem.  Next I replaced the
> hematoxylin with fresh
> (not supposed to have to change before 2500 slides,
> but changed at 1500
> slides to see if that would correct the problem). It
> has not.  
> 
> I was sure that fresh hematoxylin (and thererfore
> correct pH) would
> rectify the problem.  What are the other usual
> suspects here?  I should
> also mention our H&E's are done on the Leica
> Autostainer. I look forward
> to receiving your collective insight! Greg
> 
> 
> 
> Greg Dobbin, R.T.
> Chief Technologist, Histology Lab
> Dept. of Laboratory Medicine,
> Queen Elizabeth Hospital,
> P.O. Box 6600
> Charlottetown, PE    C1A 8T5
> Phone: (902) 894-2337
> Fax: (902) 894-2385
> 
> There is some merit in doing the right thing rather
> badly,
> but absolutely none in doing the wrong thing
> excellently!
> 
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Akemi Allison-Tacha, BS, HT(ASCP)HTL
President
Phoenix Lab Consulting & Staffing
Tele: (925)788-0900
E-Mail: akemiat3377 <@t> yahoo.com

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This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead

This note also confirms that this email message has been
virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses.
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