[Histonet] Whole Mount Slides

[Liz Chlipala]

We have not cut whole mount prostate or bladder but we have sectioned whole mount inflated guinea pig lungs, and elk trachea on a semi-automated rotary microtome (Leica RM2145), but I suspect this would work on a standard microtome.  The largest samples we have sectioned were the elk trachea, we used embedding L's to embed the sample and used a standard block clamp not quick release, where you screw down to tighten the block in the clamp.  The largest size of block we were able to cut was 2.25 x 3 inches. We had no issues with sectioning.  The guinea pig lungs fit onto 2 x 3 slides but the elk trachea barely fit on those slides (length wise).  Since we were dealing with very large samples our processing took about a week.

Liz  

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com
 
Ship to Address:
 
Premier Laboratory, LLC
University of Colorado at Boulder
MCDB, Room A3B40
Boulder, CO 80309

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Monday, January 21, 2008 2:01 PM
To: Bull, Jennifer L.; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Whole Mount Slides

The regular Minot type (rotary) microtomes usually have a maximum of 2.5 inches block travel. For larger blocks you will need a horizontal microtome, they are just what is needed for this type of specimen.
  René J.

"Bull, Jennifer L." <jennifer.bull <@t> northwestpathology.com> wrote:
  I am looking for any information on Whole Mount Slides. Our pathologists would like to start doing them on Prostate and Bladder tissue. I have researched and located a microtome attachment from Leica and SurgiPath carries oversized cassettes and embedding molds. Does anyone have any information to add?
Processing/cutting issues? Supplies? I would like to be as informed as possible before taking the plunge............ Thank You in advance!

Jennifer Bull
Histology Supervisor
NW Pathology
Bellingham, WA
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