[Histonet] Fw: antibody validation

[Tony Henwood]

Warren,
I have been on holidays so am only now catching up.
Did you get any responses?
Here is our procedure (taken from our Manual):

Scientific Approach to the Incorporation of a New Antiserum



1.	Undertake a literature search to determine its clinical
usefulness, drawbacks, technical problems etc.

2.	Analyse the antiserum's specification sheet.

3.	Perform an `Optimum Titre' on the chosen positive control
tissue.

4.	Use the antisera (diluted optimally) to test tissues (as many as
possible) known to harbour the antigen under examination.  In the case
of tumours, also include examples of poor to well differentiated
tumours.  This will give an idea of sensitivity.

5.	Test the antisera on a range of normal tissues to test for
cross-reactions.  Determine the significance of any positives.  Test
tumours arising from the normal cells.  Further optimum titering may
solve this problem.

6.	Test the antisera on conditions/tumours that are usually
entertained in the differential diagnosis.

7.	Steps 5 and 6 will provide evidence for the antiserums
specificity.

8.	Choose a positive control that exhibits the weaker staining
(will detect loss of technique sensitivity).

9.	Summarise the above information and include it in this manual.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Warren_Eddings <@t> ssmhc.com
Sent: Friday, 11 January 2008 10:15 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Fw: antibody validation



   -----Forwarded  by Warren Eddings/=AHOKC/SSMHC on 01/10/2008 05:14PM
   -----

     To: histonet <@t> lists.utsouthwestern.edu     From: Warren
Eddings/SAHOKC/SSMHC
     Date: 01/08/2008 05:33PM
     Subjec=: antibody validation

     hello

     does anyone have a procedure for the above


     thanks

     warren_eddings <@t> ssmhc.com

 
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