[Histonet] Fw: antibody validation

Tony Henwood AnthonyH <@t> chw.edu.au
Sun Jan 20 16:30:47 CST 2008

I have been on holidays so am only now catching up.
Did you get any responses?
Here is our procedure (taken from our Manual):

Scientific Approach to the Incorporation of a New Antiserum

1.	Undertake a literature search to determine its clinical
usefulness, drawbacks, technical problems etc.

2.	Analyse the antiserum's specification sheet.

3.	Perform an `Optimum Titre' on the chosen positive control

4.	Use the antisera (diluted optimally) to test tissues (as many as
possible) known to harbour the antigen under examination.  In the case
of tumours, also include examples of poor to well differentiated
tumours.  This will give an idea of sensitivity.

5.	Test the antisera on a range of normal tissues to test for
cross-reactions.  Determine the significance of any positives.  Test
tumours arising from the normal cells.  Further optimum titering may
solve this problem.

6.	Test the antisera on conditions/tumours that are usually
entertained in the differential diagnosis.

7.	Steps 5 and 6 will provide evidence for the antiserums

8.	Choose a positive control that exhibits the weaker staining
(will detect loss of technique sensitivity).

9.	Summarise the above information and include it in this manual.


Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Warren_Eddings <@t> ssmhc.com
Sent: Friday, 11 January 2008 10:15 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Fw: antibody validation

   -----Forwarded  by Warren Eddings/=AHOKC/SSMHC on 01/10/2008 05:14PM

     To: histonet <@t> lists.utsouthwestern.edu     From: Warren
     Date: 01/08/2008 05:33PM
     Subjec=: antibody validation


     does anyone have a procedure for the above


     warren_eddings <@t> ssmhc.com



Confidentiality  Noti=ce:  This  email  message, including any
   attachments,  is  for  the sole use of t=e intended recipient(s) and
   may   contain   confidential   and  privileged  inform=ation.  Any
   unauthorized  review,  use,  disclosure  or  distribution  is  prohib
ited.  If  you  are  not  the intended recipient, please contact the
   sender  by=reply  email  and  destroy  all  copies  of  the original

Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu

This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead

This note also confirms that this email message has been
virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses.

More information about the Histonet mailing list