SPAM-LOW: [Histonet] Nuclear Staining on Prostate Biopsies

[Douglas D Deltour]

Kim,

I can use a "conventional" processor with a 1 hour protocol for prostate biopsies. It is to my understanding that the Peloris increases the heat to get the "rapid". I would try using an alternative processor if possible just to rule out processing. You may need to adjust your protocol on the Peloris. 

Another issue could be the collection or grossing of the prostate biopsies. Once these are placed on gauze or a dry paper towel the water is being removed from them. Make sure the grossing tech/PA is not doing this. It is also imperative that the biopsy is not allowed to be air dried at the time of collection. It is important to educate the physician offices. 

Douglas D. Deltour HT(ASCP)
Histology Manager
Professional Pathology Services, PC
One Science Court
Suite 200
Columbia, SC 29203
Office (803)252-1913
Fax (803)254-3262
Doug <@t> ppspath.com 
*****************************************************
PROFESSIONAL PATHOLOGY SERVICES, PC
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kim Grice
Sent: Thursday, January 17, 2008 1:12 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: SPAM-LOW: [Histonet] Nuclear Staining on Prostate Biopsies

I screamed out loud when I saw this post.  We are having the same problem!  Our pathologist has been driving me crazy with this issue and will just not accept that it has nothing to do with our protocols/processing.  We use the Peloris tissue processor (with alcohols and xylenes) and use a 1 hr protocol.  The GI biopsies we get from a local hospital (obtained in the endoscopy suite) and the prostate biopsies (obtained in the individual physician's office) are run on the same 1 hr protocol.  The GI bx's look good but we have intermittent issues with the prostate cores; they look crunchy, thick and basically disgusting under the scope.  I am convinced that this problem is totally out of our hands.  Would you agree, or does anyone have suggestions on how to get these to look better?  Thanks!

 

Kim Grice
Histology Manager 
ClariPath Laboratories
Augusta, GA 30912
706-721-9341
 
 
 
 
 
 
 

-----Original Message-----
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Sent: Thursday, January 17, 2008 1:00 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 50, Issue 24

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Today's Topics:

   1. RE: Nuclear Staining on Prostate Biopsies (Laurie Colbert)
   2. no answer at Biomeda (Snider, Vivian Deanna)
   3. RE: Nuclear staining on prostate biopsies (Johnson, Teri)
   4. RE: AE1/AE3 cross reactivity (Tarango, Mark)
   5. RE: AE1/AE3 cross reactivity (Sally Price)
   6. re: Nuclear Staining on Prostate Biopsies (djemge <@t> aol.com)
   7. Re: Nuclear Staining on Prostate Biopsies (Joe Nocito)
   8. Not negative (Kemlo Rogerson)
   9. bone picture (louise renton)
  10. Re: Not negative (Rene J Buesa)
  11. Hepsin immunohistochemistry (sotiris lakis)
  12. Re: no answer at Biomeda (Victoria Baker)
  13. RE: AE1/AE3 cross reactivity (Mighnon Lashus)
  14. RE: AE1/AE3 cross reactivity (sheila adey)
  15. fixation and processing whole human eyes (gregor <@t> arlt-digital.de)
  16. Looking for chondrocyte Ab (Larry Woody)


----------------------------------------------------------------------

Message: 1
Date: Wed, 16 Jan 2008 10:17:30 -0800
From: "Laurie Colbert" <laurie.colbert <@t> huntingtonhospital.com>
Subject: RE: [Histonet] Nuclear Staining on Prostate Biopsies
To: "Laurie Elmgren" <lelmgren <@t> sunriselab.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<57BE698966D5C54EAE8612E8941D76830248A4B6 <@t> EXCHANGE3.huntingtonhospital.com>
	
Content-Type: text/plain;	charset="us-ascii"

One of the biggest problems that we encounter is that the prostate
biopsies are drying out at the office before they get put into the
formalin.  One office in particular places the specimens on gauze before
they go into formalin.  I think they obtain all of the specimens and
then put them all into the bottles of formalin last.  We get bad
staining, crunchy, dry specimens, etc.  If you are having problems with
a particular office, talk to them and make sure the tissue is going into
formalin immediately after it is removed from the patient.

Laurie Colbert

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Laurie
Elmgren
Sent: Wednesday, January 16, 2008 9:43 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Nuclear Staining on Prostate Biopsies

I would like to optimize the quality of our stain.  We process office
biopsies, a good variety of tissue types.

The stain is consistently very good. There are three colors of Eosin,
and good nuclear detail on most tissue types, except for the prostate
cores.

The nuclei are stained dark and are "flat" having no depth.

I am thinking that it is not the fixation, because our Monday specimens
that are held over a full day are no different than those throughout the
week.

Any suggestions before I start reprogramming the stainer?

 

Laurie Elmgren

Histology Supervisor

Sunrise Medical Labs

240 Motor Pkwy

Hauppauge, NY 11788

(631)435-1515-x1108

 

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------------------------------

Message: 2
Date: Wed, 16 Jan 2008 13:17:56 -0500
From: "Snider, Vivian Deanna" <vsnider <@t> shrinenet.org>
Subject: [Histonet] no answer at Biomeda
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<84BE46B37B314D409C5A17B7BAB022D601BF9C21 <@t> IDC-EX-VS01.shriners.cc>
Content-Type: text/plain;	charset="us-ascii"

I have been having some issues with Biomeda also. It has been almost a
month and I still have not received the product, ordered through Fisher.
I am told it is a manufacturer's backorder.  I am currently researching
a secondary source for the product.

 

Deanna Snider HT ASCP

Lead Histotechnician

Shriners' Hospital for Children

Research Dept.

Cincinnati, OH

 



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------------------------------

Message: 3
Date: Wed, 16 Jan 2008 12:56:20 -0600
From: "Johnson, Teri" <TJJ <@t> Stowers-Institute.org>
Subject: [Histonet] RE: Nuclear staining on prostate biopsies
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<C28BAF593DC3314E9C0F3A50191C2E7807A3E93A <@t> EXCHKC03.stowers-institute.org>
	
Content-Type: text/plain;	charset="us-ascii"

Laurie, contact the operating room (or doctor's office) staff and ask
them how the samples are handled when they are released from the biopsy
gun. My guess is they are put on dry gauze and some air drying is taking
place prior to being fixed. Or they are placed in some other medium
besides fixative immediately. Many times the damage is already done
before we ever receive the specimen. It's just a guess on my part, but
mostly I think it's because all the other tissue types look good with
your processing and staining times.

Good luck!

Teri Johnson, HT(ASCP)QIHC
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, MO 64110



>>Laurie wrote: I would like to optimize the quality of our stain.  We
process office biopsies, a good variety of tissue types.

The stain is consistently very good. There are three colors of Eosin,
and good nuclear detail on most tissue types, except for the prostate
cores.

The nuclei are stained dark and are "flat" having no depth.

I am thinking that it is not the fixation, because our Monday specimens
that are held over a full day are no different than those throughout the
week.

Any suggestions before I start reprogramming the stainer?

 

Laurie Elmgren

Histology Supervisor

Sunrise Medical Labs

240 Motor Pkwy

Hauppauge, NY 11788

(631)435-1515-x1108





------------------------------

Message: 4
Date: Wed, 16 Jan 2008 12:45:14 -0800
From: "Tarango, Mark" <mtarango <@t> nvcancer.org>
Subject: RE: [Histonet] AE1/AE3 cross reactivity
To: "Richard Hessler, M.D." <rhessler <@t> pathgroup.com>,
	histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<5AEC610C1CE02945BD63A395BA763EDE01E48CDE <@t> NVCIEXCH02.NVCI.org>
Content-Type: text/plain; charset=us-ascii

I noticed the plasma cell staining just yesterday.  We use Ventana's
pancytokeratin cocktail (AE1/AE3/PCK26).  I was using LU-5 for
pankeratin previously, but had problems with that antibody (no
staining).

Mark Adam Tarango HT(ASCP)QIHC
Histology & IHC Supervisor
Nevada Cancer Institute
One Breakthrough Way
Las Vegas, NV 89135
Direct Line (702) 822-5112
Treo (702) 759-9229
Fax (702) 939-7663

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Richard
Hessler, M.D.
Sent: Wednesday, January 16, 2008 5:58 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] AE1/AE3 cross reactivity

I have used this antibody for years but now have issues. Previously it
was the best choice for breast sentinel nodes, but now AE1/AE3  stains
plasma cells like CD138. It also stains a number of different sarcomas
and beautifully highlights reactive astrocytes. I had previously used
Dako Ab and autostainer with no problems. Now I have Ventanna and have
noticed this with both Ventana and Dako Abs used on that system.

Any thoughts???

Richard B Hessler, MD
Chief of Pathology
Erlanger Medical Center
Chattanooga, TN



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"EMF <nvcancer.org>" made the following annotations.
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Message: 5
Date: Wed, 16 Jan 2008 17:52:30 -0500
From: "Sally Price" <sprice2003 <@t> gmail.com>
Subject: RE: [Histonet] AE1/AE3 cross reactivity
To: rhessler <@t> pathgroup.com, histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<ffd26fa80801161452od1dcb91v324a9ea0634b89b4 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Dr. Hessler:
I have observed the phenomenon that you've described for other antibodies
more times than I can remember.  Personally, I believe that it has to do
with the way the Ventana system sprays on the retrieval solution
and heats slides during antibody/detection incubation periods, which is too
aggressive.  I think that an open system that doesn't heat slides or perform
AR isn't likely to produce these unexpalined reactions, but I digress.  So,
how do you fix the problem?  I say disable the on-line AR, and perform it in
a decloaking chamber, set at 100 degrees for 10 minutes.  Or, you could
consider using another pan-CK antibody, like Lu-5.
Good Luck,
Sally Price

----------------------------------------------------------------------------------------------

Date: Wed, 16 Jan 2008 07:57:41 -0600
From: "Richard Hessler, M.D." <rhessler <@t> pathgroup.com>
Subject: [Histonet] AE1/AE3 cross reactivity
To: "histonet <@t> lists.utsouthwestern.edu"
    <histonet <@t> lists.utsouthwestern.edu>
"
I have used this antibody for years but now have issues. Previously it was
the best choice for breast sentinel nodes, but now AE1/AE3  stains plasma
cells like CD138. It also stains a number of different sarcomas and
beautifully highlights reactive astrocytes. I had previously used Dako Ab
and autostainer with no problems. Now I have Ventanna and have noticed this
with both Ventana and Dako Abs used on that system.

Any thoughts???

Richard B Hessler, MD
Chief of Pathology
Erlanger Medical Center
Chattanooga, TN


------------------------------

Message: 6
Date: Wed, 16 Jan 2008 18:54:45 -0500
From: djemge <@t> aol.com
Subject: [Histonet] re: Nuclear Staining on Prostate Biopsies
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <8CA26B24E3B1125-8AC-4B4 <@t> MBLK-M08.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"

This dark nuclei is often seen when tissue is left to dry for a few minutes before being fixed, or?if underfixed and?the alcohols on the processor fix the tissue. Since you are sure it is not dried or underfixed then it has to be the processing schedule. I agree with Renee. This sounds like a processing problem. Sounds like the overall schedule is too long for these biopsies. Especially if too long in paraffin.
Donna

Donna Emge HT-ASCP
Northwestern University
Feinburg School of Medicine
303 E. Superior, Lurie 7-220
Chicago, IL 60611
312-503-2036
d-emge <@t> northwestern.edu
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------------------------------

Message: 7
Date: Wed, 16 Jan 2008 17:58:28 -0600
From: "Joe Nocito" <jnocito <@t> satx.rr.com>
Subject: Re: [Histonet] Nuclear Staining on Prostate Biopsies
To: "Laurie Elmgren" <lelmgren <@t> sunriselab.com>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <004601c8589b$c949c7d0$0302a8c0 <@t> yourxhtr8hvc4p>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
	reply-type=original

Laurie,
at my last place, we had to set up a different staining program for the 
reasons you stated. We decreased the hematoxylin and differentiation times, 
increased the bluing time and increased the wash time. I set up testing as 
follows:

Group 1: hema  at 30 second intervals 2:00, 2:30, 3:00, 3:30 , everything 
else stayed the same (4 slides)
Group 2 : hema  at 30 second intervals , but increase bluing 15 second 
intervals 30, 45, 60, 75 seconds, everything else stayed the same (16 
slides)
Group 3: hema 30 second intervals, bluing 15 second intervals and 
differentiation time by 15 seconds (64 slides, I think)

Lot of work, no doubt, but that's what the pathologists wanted. Have fun and 
good luck

JTT


----- Original Message ----- 
From: "Laurie Elmgren" <lelmgren <@t> sunriselab.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, January 16, 2008 11:42 AM
Subject: [Histonet] Nuclear Staining on Prostate Biopsies


I would like to optimize the quality of our stain.  We process office
biopsies, a good variety of tissue types.

The stain is consistently very good. There are three colors of Eosin,
and good nuclear detail on most tissue types, except for the prostate
cores.

The nuclei are stained dark and are "flat" having no depth.

I am thinking that it is not the fixation, because our Monday specimens
that are held over a full day are no different than those throughout the
week.

Any suggestions before I start reprogramming the stainer?



Laurie Elmgren

Histology Supervisor

Sunrise Medical Labs

240 Motor Pkwy

Hauppauge, NY 11788

(631)435-1515-x1108



_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 




------------------------------

Message: 8
Date: Thu, 17 Jan 2008 08:51:20 -0000
From: "Kemlo Rogerson" <Kemlo.Rogerson <@t> waht.swest.nhs.uk>
Subject: [Histonet] Not negative
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<86ADE4EB583CE64799A9924684A0FBBF0222F4BB <@t> wahtntex2.waht.swest.nhs.uk>
Content-Type: text/plain;	charset="us-ascii"

I think we had a discussion last week about Pathologists reporting
negative slides; interestingly there's a television news story that just
broken about a Welsh Pathologist wrongly reporting biopsies. I gather
there are a number of biopsies that were initially reported as negative
in which the Patients subsequently developed cancer; or that's what I
think the story said.

This Pathologists work over a number of years is to be reviewed; reminds
me of the bad years in Cytology. Abnormal slides are peer reviewed but
not 'negative' ones which is odd; if you diagnosed something then the
Patient will be treated (or further investigated which might flag up
errors), if you call something negative then the Patient may have no
treatment unless the symptoms remain and further tests reveal the
abnormality. How gold is the gold standard of tissue diagnosis?

 
Kemlo Rogerson        
Pathology Manager
DD   01934 647057 or extension 3311
Mob 07749 754194; Pager 07659 597107;
Don't be afraid to take a big step when one is indicated. You can't
cross a chasm in two small jumps. --Buckminster Fuller 

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------------------------------

Message: 9
Date: Thu, 17 Jan 2008 13:37:20 +0200
From: "louise renton" <louise.renton <@t> gmail.com>
Subject: [Histonet] bone picture
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<e483362e0801170337h743f2eadj6de90342cd53ed66 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Hi all, is anyone willing to share a low power view of an undecalcified MMA
bone section?? I desperately need one for a talk I am giving and our camera
has gone all funny and won't take low power pictures...any help at all,
please?????

best regards-
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
"There are nights when the wolves are silent and only the moon howls".
George Carlin
No trees were killed in the sending of this message.
However, many electrons were terribly inconvenienced.


------------------------------

Message: 10
Date: Thu, 17 Jan 2008 06:19:34 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Not negative
To: Kemlo Rogerson <Kemlo.Rogerson <@t> waht.swest.nhs.uk>,
	histonet <@t> lists.utsouthwestern.edu
Message-ID: <540032.28982.qm <@t> web61221.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

This reinforces my view that a "false negative" is always worse that a "false positive" although some pathlogists disagree.
  With a "false negative" you do nothing and with a "false positive" you do something even if it is not necessary, you err in the "cautious side".
  René J.

Kemlo Rogerson <Kemlo.Rogerson <@t> waht.swest.nhs.uk> wrote:
  
 



       
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------------------------------

Message: 11
Date: Thu, 17 Jan 2008 14:51:58 +0000 (GMT)
From: sotiris lakis <sotlak <@t> yahoo.gr>
Subject: [Histonet] Hepsin immunohistochemistry
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <455401.58701.qm <@t> web23001.mail.ird.yahoo.com>
Content-Type: text/plain; charset=iso-8859-7

Hello everybody,
  I am interested in performing immunohistochemistry with hepsin on paraffin sections from prostate carcinoma tissue. I would be greatful if somebody who has already tested any of the antibodies commercially availiable would give me a hint about what to expect.
  Thank you in advance
                                                 Sotiris Lakis 

       
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------------------------------

Message: 12
Date: Thu, 17 Jan 2008 09:53:05 -0500
From: "Victoria Baker" <bakevictoria <@t> gmail.com>
Subject: Re: [Histonet] no answer at Biomeda
To: "Snider, Vivian Deanna" <vsnider <@t> shrinenet.org>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<4f016b690801170653s605768c1q77874a796f6a683f <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

I had a situation a couple of years back where I was purchasing
supplies through Fisher and also experienced LONG waits for receipt of
products.  It turned out that in some instances Fisher has to go
through another distributor as the direct manufacturer will not sell
direct to Fisher.  In the case of Biomeda, I wouldn't think this is
the case however I'd contact your Fisher rep and have him investigate
what is happening.  With all the mergers/buy-outs and switch outs it's
hard to keep track of who owns what that will sell to a certain
distributor that will in the end finally get you the product you
requested before you've retired or gone on to the great reward.

I'm a firm believer in making the sales rep earn 'their keep' as they
need your business, and if your rep isn't helping go to their boss.



On 1/16/08, Snider, Vivian Deanna <vsnider <@t> shrinenet.org> wrote:
> I have been having some issues with Biomeda also. It has been almost a
> month and I still have not received the product, ordered through Fisher.
> I am told it is a manufacturer's backorder.  I am currently researching
> a secondary source for the product.
>
>
>
> Deanna Snider HT ASCP
>
> Lead Histotechnician
>
> Shriners' Hospital for Children
>
> Research Dept.
>
> Cincinnati, OH
>
>
>
>
>
> CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients. If you are not the intended recipient, (or authorized to receive for the recipient) you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please destroy all copies of this communication and any attachments and contact the sender by reply e-mail or telephone (813) 281-0300.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



------------------------------

Message: 13
Date: Thu, 17 Jan 2008 09:52:03 -0600
From: Mighnon Lashus <MLashus <@t> pathgroup.com>
Subject: RE: [Histonet] AE1/AE3 cross reactivity
To: Sally Price <sprice2003 <@t> gmail.com>,
	"histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<197CD0B02A81F94994A285C59C8AE05C023B419A2B <@t> pgnexchange.pathgroup.com>
Content-Type: text/plain; charset="us-ascii"

We do not use AR for the AE1/AE3 on our Ventana system.  We use Protease 1 for 4 minutes.  I will check into the heat.  Any other suggestions?  We do the stains for Dr. Hessler.
Thanks,
Mighnon Lashus

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sally Price
Sent: Wednesday, January 16, 2008 5:53 PM
To: Richard Hessler, M.D.; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] AE1/AE3 cross reactivity

Dr. Hessler:
I have observed the phenomenon that you've described for other antibodies
more times than I can remember.  Personally, I believe that it has to do
with the way the Ventana system sprays on the retrieval solution
and heats slides during antibody/detection incubation periods, which is too
aggressive.  I think that an open system that doesn't heat slides or perform
AR isn't likely to produce these unexpalined reactions, but I digress.  So,
how do you fix the problem?  I say disable the on-line AR, and perform it in
a decloaking chamber, set at 100 degrees for 10 minutes.  Or, you could
consider using another pan-CK antibody, like Lu-5.
Good Luck,
Sally Price

----------------------------------------------------------------------------------------------

Date: Wed, 16 Jan 2008 07:57:41 -0600
From: "Richard Hessler, M.D." <rhessler <@t> pathgroup.com>
Subject: [Histonet] AE1/AE3 cross reactivity
To: "histonet <@t> lists.utsouthwestern.edu"
    <histonet <@t> lists.utsouthwestern.edu>
"
I have used this antibody for years but now have issues. Previously it was
the best choice for breast sentinel nodes, but now AE1/AE3  stains plasma
cells like CD138. It also stains a number of different sarcomas and
beautifully highlights reactive astrocytes. I had previously used Dako Ab
and autostainer with no problems. Now I have Ventanna and have noticed this
with both Ventana and Dako Abs used on that system.

Any thoughts???

Richard B Hessler, MD
Chief of Pathology
Erlanger Medical Center
Chattanooga, TN
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Message: 14
Date: Thu, 17 Jan 2008 11:08:41 -0500
From: sheila adey <sheila_adey <@t> hotmail.com>
Subject: RE: [Histonet] AE1/AE3 cross reactivity
To: Mighnon Lashus <mlashus <@t> pathgroup.com>, Sally Price
	<sprice2003 <@t> gmail.com>, "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
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Our Dr.s have stopped ordering the AE1/AE3 ab aswell. For the same complaints. We use the Biogenex abs.
Sheila Adey HT MLTPort Huron HospitalMichigan> From: MLashus <@t> pathgroup.com> To: sprice2003 <@t> gmail.com; histonet <@t> lists.utsouthwestern.edu> Date: Thu, 17 Jan 2008 09:52:03 -0600> Subject: RE: [Histonet] AE1/AE3 cross reactivity> CC: > > We do not use AR for the AE1/AE3 on our Ventana system. We use Protease 1 for 4 minutes. I will check into the heat. Any other suggestions? We do the stains for Dr. Hessler.> Thanks,> Mighnon Lashus> > -----Original Message-----> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sally Price> Sent: Wednesday, January 16, 2008 5:53 PM> To: Richard Hessler, M.D.; histonet <@t> lists.utsouthwestern.edu> Subject: RE: [Histonet] AE1/AE3 cross reactivity> > Dr. Hessler:> I have observed the phenomenon that you've described for other antibodies> more times than I can remember. Personally, I believe that it has to do> with the way the Ventana system sprays on the retrieval solution> and heats slides during antibody/detection incubation periods, which is too> aggressive. I think that an open system that doesn't heat slides or perform> AR isn't likely to produce these unexpalined reactions, but I digress. So,> how do you fix the problem? I say disable the on-line AR, and perform it in> a decloaking chamber, set at 100 degrees for 10 minutes. Or, you could> consider using another pan-CK antibody, like Lu-5.> Good Luck,> Sally Price> > ----------------------------------------------------------------------------------------------> > Date: Wed, 16 Jan 2008 07:57:41 -0600> From: "Richard Hessler, M.D." <rhessler <@t> pathgroup.com>> Subject: [Histonet] AE1/AE3 cross reactivity> To: "histonet <@t> lists.utsouthwestern.edu"> <histonet <@t> lists.utsouthwestern.edu>> "> I have used this antibody for years but now have issues. Previously it was> the best choice for breast sentinel nodes, but now AE1/AE3 stains plasma> cells like CD138. It also stains a number of different sarcomas and> beautifully highlights reactive astrocytes. I had previously used Dako Ab> and autostainer with no problems. Now I have Ventanna and have noticed this> with both Ventana and Dako Abs used on that system.> > Any thoughts???> > Richard B Hessler, MD> Chief of Pathology> Erlanger Medical Center> Chattanooga, TN> _______________________________________________> Histonet mailing list> Histonet <@t> lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you> > _______________________________________________> Histonet mailing list> Histonet <@t> lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Message: 15
Date: Thu, 17 Jan 2008 17:08:57 +0100
From: gregor <@t> arlt-digital.de
Subject: [Histonet] fixation and processing whole human eyes
To: <histonet <@t> lists.utsouthwestern.edu>
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Content-Type: text/plain; charset=UTF-8

Hi All,

 
I’m looking for a protocol to process whole human eyes in a vacuum infiltration system. Could anybody help with this?


 Thanks a lot.

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Message: 16
Date: Thu, 17 Jan 2008 09:43:32 -0800 (PST)
From: Larry Woody <slappycraw <@t> yahoo.com>
Subject: [Histonet] Looking for chondrocyte Ab
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <625394.77121.qm <@t> web53604.mail.re2.yahoo.com>
Content-Type: text/plain; charset=us-ascii

That will bind with chondrocytes in formalin fixed paraffin embedded mouse joint tissues. Any info would be appreciated. Thanks.
 
Larry A. Woody
Seattle, Wa.


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End of Histonet Digest, Vol 50, Issue 24
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