[Histonet] AE1/AE3 cross reactivity

Tarango, Mark mtarango <@t> nvcancer.org
Wed Jan 16 14:45:14 CST 2008


I noticed the plasma cell staining just yesterday.  We use Ventana's
pancytokeratin cocktail (AE1/AE3/PCK26).  I was using LU-5 for
pankeratin previously, but had problems with that antibody (no
staining).

Mark Adam Tarango HT(ASCP)QIHC
Histology & IHC Supervisor
Nevada Cancer Institute
One Breakthrough Way
Las Vegas, NV 89135
Direct Line (702) 822-5112
Treo (702) 759-9229
Fax (702) 939-7663

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Richard
Hessler, M.D.
Sent: Wednesday, January 16, 2008 5:58 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] AE1/AE3 cross reactivity

I have used this antibody for years but now have issues. Previously it
was the best choice for breast sentinel nodes, but now AE1/AE3  stains
plasma cells like CD138. It also stains a number of different sarcomas
and beautifully highlights reactive astrocytes. I had previously used
Dako Ab and autostainer with no problems. Now I have Ventanna and have
noticed this with both Ventana and Dako Abs used on that system.

Any thoughts???

Richard B Hessler, MD
Chief of Pathology
Erlanger Medical Center
Chattanooga, TN



________________________________
Important Notice: This e-mail is intended for the use of the person to
whom it is addressed and may contain information that is privileged and
confidential. If you are not the intended recipient, any disclosure,
copying, distribution, or use of the contents of this message is
strictly prohibited. If you have received this e-mail in error, please
destroy this message and contact the Security Officer at PathGroup, Inc
immediately at 615-562-9255. Thank you
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


"EMF <nvcancer.org>" made the following annotations.
------------------------------------------------------------------------------
CONFIDENTIALITY NOTICE: This e-mail message, including any
attachments, is for the sole use of the intended 
recipient(s) and may contain confidential, proprietary, 
and/or privileged information protected by law. If you are 
not the intended recipient, you may not use, copy, or 
distribute this e-mail message or its attachments. If you 
believe you have received this e-mail message in error, 
please contact the sender by reply e-mail and destroy all 
copies of the original message
==============================================================================




More information about the Histonet mailing list