[Histonet] AE1/AE3 cross reactivity

[Tarango, Mark]

I noticed the plasma cell staining just yesterday.  We use Ventana's
pancytokeratin cocktail (AE1/AE3/PCK26).  I was using LU-5 for
pankeratin previously, but had problems with that antibody (no
staining).

Mark Adam Tarango HT(ASCP)QIHC
Histology & IHC Supervisor
Nevada Cancer Institute
One Breakthrough Way
Las Vegas, NV 89135
Direct Line (702) 822-5112
Treo (702) 759-9229
Fax (702) 939-7663

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Richard
Hessler, M.D.
Sent: Wednesday, January 16, 2008 5:58 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] AE1/AE3 cross reactivity

I have used this antibody for years but now have issues. Previously it
was the best choice for breast sentinel nodes, but now AE1/AE3  stains
plasma cells like CD138. It also stains a number of different sarcomas
and beautifully highlights reactive astrocytes. I had previously used
Dako Ab and autostainer with no problems. Now I have Ventanna and have
noticed this with both Ventana and Dako Abs used on that system.

Any thoughts???

Richard B Hessler, MD
Chief of Pathology
Erlanger Medical Center
Chattanooga, TN



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"EMF <nvcancer.org>" made the following annotations.
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