[Histonet] Need the evg/evg procedure question below

Madary, Joseph MadaryJ <@t> MedImmune.com
Tue Jan 15 10:50:16 CST 2008


 We moved and my old books sprouted legs.  I want to do the elastic
stain using the verhoeffs hema(the one that uses ferric chloride) and
then differnetiates in 2% ferric chloride after that.  I do not have
resorcin or orcein and my aldehyde fuchsin is old so I am trying tio use
what I know I have on hand to get this done now instead of waiting.  I
want to say evg/vvg.


Nick Madary, HT/HTL(ASCP)QIHC
Medimmune Histology Laboratory
One Medimmune Way
Gaithersburg, MD 20878

ph 301.398.4745/6360
fx  301.398.9745

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
histonet-request <@t> lists.utsouthwestern.edu
Sent: Monday, January 14, 2008 1:12 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 50, Issue 21

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Today's Topics:

   1. Aurora (Pete and Sharon Jack)
   2. Re: Who can perform Histology Duties (Esther Peters)
   3. RBC removal (Min-Han Tan)
   4. Re: BrdU/DCX problem (Olek Michalski)
   5. RE: Who can perform Histology Duties
      (Marshall Terry Dr,	Consultant Histopathologist)
   6. RELIA Histology Opportunity Update 01-14-08 Happy New	Year!
      (Pam Barker)


----------------------------------------------------------------------

Message: 1
Date: Sun, 13 Jan 2008 15:29:29 -0500
From: "Pete and Sharon Jack" <babears <@t> triad.rr.com>
Subject: [Histonet] Aurora
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <001101c85622$ffd90a70$90e84c47 <@t> pete38d56c4d11>
Content-Type: text/plain;	charset="iso-8859-1"

Our laboratory was purchased by Auror on October 2, 2007.
It's too soon to tell how things will be.  We did lose our profit
sharing which was quite lucrative.
Sharon S. Jack, HT(ASCP)

------------------------------

Message: 2
Date: Sun, 13 Jan 2008 17:22:13 -0500
From: Esther Peters <esther.peters <@t> verizon.net>
Subject: Re: [Histonet] Who can perform Histology Duties
To: "Marshall Terry Dr, Consultant Histopathologist"
	<Terry.Marshall <@t> rothgen.nhs.uk>
Cc: histonet <@t> lists.utsouthwestern.edu, Terri Braud
	<tbraud <@t> holyredeemer.com>,	Kemlo Rogerson
	<Kemlo.Rogerson <@t> waht.swest.nhs.uk>
Message-ID: <478A8F15.5050904 <@t> verizon.net>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed

Terry,

Enjoyed your comments.  Training, continuing education, and especially
knowing when you should refer to someone else are key in any profession!

Regarding "norma,l" the pathologists are not using "some sort of mealy
mouthed evasion" but they know that there is a "range" of normalcy when
identifying the condition of cells and tissues, because of individual
variation, age, reproductive status, nutritional status, etc.  Just
think of the wide range in values for what are considered "nondiseased" 
levels of various blood parameters.  I once corresponded with a
professor of histology, who told me he only knew "normal" and didn't
know anything about pathology.  But if you don't understand structural
changes indicating diseases, are you sure you can recognize "normal"?

Esther Peters, Ph.D.
George Mason University

Marshall Terry Dr, Consultant Histopathologist wrote:
>  "Proper training can allow Histotech's to report normal, ....."
>
> There was a bizarre discussion on the histopathology discussion group 
> recently, around reporting tissue as "normal".
> An amazing number of pathologists never used the word, but preferred 
> some sort of mealy mouthed evasion.
> Within normal limits, no definite lesion seen ... That sort of thing.
> Indeed one very competent pathologist claimed he could not recognise 
> normal!
>
> Terry
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kemlo 
> Rogerson
> Sent: 10 January 2008 16:08
> To: Terri Braud; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Who can perform Histology Duties
>
> Please save your flames.  The scenerio of a Non-Histotech embedding, 
> cutting, and staining my surgical tissue does not thrill me, but that 
> was not her question.  Maybe the reality of the answer is why many 
> Histology Labs have always been considered the evil red-headed 
> stepchildren of the Lab.
> Terri
>
> I think you are correct in that as long as someone is properly 
> trained, the training is revisited regularly and there is adequate 
> supervision and the professional groups are in agreement Non-BMS 
> (Non-Histotech) Staff can do many jobs in Histology and maybe Non 
> Medical Staff (BMS) can carry out some interpretation. I would have no

> issues with a Non-Histotech (Non-BMS) doing as you say with proper 
> supervision by a HPC registered supervisor.
>
> But where does it end? Do you need a medical degree to identify vas, 
> appendix or gall bladder? You need the training to know when you have 
> reached the limits of your knowledge and when to refer to someone 
> better able to report a disease and I concede that is probably a 
> Medic. Proper training can allow Histotech's to report normal, maybe 
> non-normal requires medical insight, what do you think?
>
>  
> Kemlo Rogerson        
> Pathology Manager
> DD   01934 647057 or extension 3311
> Mob 07749 754194; Pager 07659 597107;
> Don't be afraid to take a big step when one is indicated. You can't 
> cross a chasm in two small jumps. --Buckminster Fuller
>
> This e-mail is confidential and privileged. If you are not the 
> intended recipient please accept my apologies; please do not disclose,

> copy or distribute information in this e-mail or take any action in 
> reliance on its contents: to do so is strictly prohibited and may be
unlawful.
> Please inform me that this message has gone astray before deleting it.
> Thank you for your co-operation
>  
>
>
> _______________________________________________
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>   



------------------------------

Message: 3
Date: Mon, 14 Jan 2008 13:58:13 +0800
From: "Min-Han Tan" <minhan.science <@t> gmail.com>
Subject: [Histonet] RBC removal
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<7902152a0801132158x2be7da3dxb0649bd086c4f25d <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Dear fellow list members,

I have been trying to remove RBCs from bloody pleural effusions /
abdominal ascites using Histopaque, with low success, for the purpose of
cytology. Histopaque works great on blood.

I wonder if any one has successfully worked on bloody effusions /
ascites previously; they do not seem to centrifuge in as optimal a
fashion as blood, and I wonder if there are proteins / free hemoglobin
that may be altering the density somewhat. Has anyone used ACK buffer or
any other such methods to remove / lyse RBCs?

Thanks!

Regards,
Min-Han Tan



------------------------------

Message: 4
Date: Mon, 14 Jan 2008 07:39:16 +0100
From: "Olek Michalski" <olek.michalski <@t> nencki.gov.pl>
Subject: Re: [Histonet] BrdU/DCX problem
To: "Jonathan Frank" <jfrank <@t> med.unc.edu>
Cc: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <op.t4wp3qgs445ael <@t> pc-313-1.ibd>
Content-Type: text/plain; format=flowed; delsp=yes; charset=iso-8859-2

Dear Jon,

2008-01-10 19:11:20 Jonathan Frank <jfrank <@t> med.unc.edu> wrote:

[...]
> Thanks for the response.  Do you do 1st primary, 1st secondary, then 
> brdU primary, and BrdU secondary?
[...]

It is exactly the case. If you like I can ask my coleague, who actually
worked out the protocol and uses it routinely for a detailed list of
opertions with some comments. At first glance I see some differences in
BrdU labelling procedure.

> Also, which BrdU primary antibody do you use?

For BrdU we use antibody from Roche. I don't remember the catalogue no.

and the characteristics I can found at the moment is a bit old but I
suppose they offer only one kind.

Best regards
Olek
-- 
        Laboratory of Neurobiology
       of Development and Evolution

  Nencki Institute of Experimental Biology
  ul. Pasteura 3, 02-093 Warszawa,  Poland
  Tel. +48 22 5892268,  Fax +48 22 8225342




------------------------------

Message: 5
Date: Mon, 14 Jan 2008 12:26:00 -0000
From: "Marshall Terry Dr,	Consultant Histopathologist"
	<Terry.Marshall <@t> rothgen.nhs.uk>
Subject: RE: [Histonet] Who can perform Histology Duties
To: "Esther Peters" <esther.peters <@t> verizon.net>
Cc: histonet <@t> lists.utsouthwestern.edu, Terri Braud
	<tbraud <@t> holyredeemer.com>,	Kemlo Rogerson
	<Kemlo.Rogerson <@t> waht.swest.nhs.uk>
Message-ID:
	
<5C0BED61F529364E86309CADEA63FEF2F3AF41 <@t> TRFT-EX01.xRothGen.nhs.uk>
Content-Type: text/plain;	charset="US-ASCII"

Odd that there should be only one comment on my post, because I thought
it (the subject rather than my post) was an interesting one. It's
impossible to judge just what gets people interested.
Anyway, back at the ranch, you are right that there is a range of
appearances, but that range is only of significance in breast and
prostate, of the things we see commonly, and it would certainly be true
that "normal" has very fuzzy edges in these organs.
However, for the rest, there is no significant range.
Large bowel biopsies actually gives rise to problems too, as they are
almost always abnormal, but only mildly so, and unfortunately, not
showing any features by which one can make a diagnosis. I usually report
these as no significant abnormality, or similar, but sometimes I just
call them normal, as it really makes no practical difference. As these
patients have bowel problems, usually diarrhoea, and many have had some
sort of bowel prep., it is no surprise that the appearance should not be
"normal" in the sense of ideal.

It's all a matter of how you look at things, and in US in particular,
how well you like to cover your ass.

Terry

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Esther
Peters
Sent: 13 January 2008 22:22
To: Marshall Terry Dr, Consultant Histopathologist
Cc: histonet <@t> lists.utsouthwestern.edu; Terri Braud; Kemlo Rogerson
Subject: Re: [Histonet] Who can perform Histology Duties

Terry,

Enjoyed your comments.  Training, continuing education, and especially
knowing when you should refer to someone else are key in any profession!

Regarding "norma,l" the pathologists are not using "some sort of mealy
mouthed evasion" but they know that there is a "range" of normalcy when
identifying the condition of cells and tissues, because of individual
variation, age, reproductive status, nutritional status, etc.  Just
think of the wide range in values for what are considered "nondiseased" 
levels of various blood parameters.  I once corresponded with a
professor of histology, who told me he only knew "normal" and didn't
know anything about pathology.  But if you don't understand structural
changes indicating diseases, are you sure you can recognize "normal"?

Esther Peters, Ph.D.
George Mason University

Marshall Terry Dr, Consultant Histopathologist wrote:
>  "Proper training can allow Histotech's to report normal, ....."
>
> There was a bizarre discussion on the histopathology discussion group 
> recently, around reporting tissue as "normal".
> An amazing number of pathologists never used the word, but preferred 
> some sort of mealy mouthed evasion.
> Within normal limits, no definite lesion seen ... That sort of thing.
> Indeed one very competent pathologist claimed he could not recognise 
> normal!
>
> Terry
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kemlo 
> Rogerson
> Sent: 10 January 2008 16:08
> To: Terri Braud; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Who can perform Histology Duties
>
> Please save your flames.  The scenerio of a Non-Histotech embedding, 
> cutting, and staining my surgical tissue does not thrill me, but that 
> was not her question.  Maybe the reality of the answer is why many 
> Histology Labs have always been considered the evil red-headed 
> stepchildren of the Lab.
> Terri
>
> I think you are correct in that as long as someone is properly 
> trained, the training is revisited regularly and there is adequate 
> supervision and the professional groups are in agreement Non-BMS
> (Non-Histotech) Staff can do many jobs in Histology and maybe Non 
> Medical Staff (BMS) can carry out some interpretation. I would have no

> issues with a Non-Histotech (Non-BMS) doing as you say with proper 
> supervision by a HPC registered supervisor.
>
> But where does it end? Do you need a medical degree to identify vas, 
> appendix or gall bladder? You need the training to know when you have 
> reached the limits of your knowledge and when to refer to someone 
> better able to report a disease and I concede that is probably a 
> Medic. Proper training can allow Histotech's to report normal, maybe 
> non-normal requires medical insight, what do you think?
>
>  
> Kemlo Rogerson        
> Pathology Manager
> DD   01934 647057 or extension 3311
> Mob 07749 754194; Pager 07659 597107;
> Don't be afraid to take a big step when one is indicated. You can't 
> cross a chasm in two small jumps. --Buckminster Fuller
>
> This e-mail is confidential and privileged. If you are not the 
> intended recipient please accept my apologies; please do not disclose,

> copy or distribute information in this e-mail or take any action in 
> reliance on its contents: to do so is strictly prohibited and may be
unlawful.
> Please inform me that this message has gone astray before deleting it.
> Thank you for your co-operation
>  
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> ######################################################################
> This email and any files transmitted with it may contain confidential
and privileged information which is intended solely for the use of the
individual or entity to whom they are addressed. Any views or opinions
expressed are those of the author and do not represent the views of The
Rotherham NHS Foundation Trust unless otherwise explicitly stated.
> The information contained in this email may be subject to public
disclosure under the Freedom of Information Act 2000 unless the
information is legally exempt from disclosure. The confidentiality of
this email and your reply cannot be guaranteed.
> If you are not the intended recipient please accept our apologies.
Please do not disclose, copy or distribute information in this email or
take any action in reliance on its contents: to do so is strictly
prohibited and may be unlawful. Please inform us that this message has
gone astray before deleting it. Thank you for your co-operation.
> ######################################################################
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>   

_______________________________________________
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------------------------------

Message: 6
Date: Mon, 14 Jan 2008 09:01:10 -0500
From: "Pam Barker" <relia1 <@t> earthlink.net>
Subject: [Histonet] RELIA Histology Opportunity Update 01-14-08 Happy
	New	Year!
To: "'Histonet'" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <E1JEPsK-0002oE-84 <@t> elasmtp-junco.atl.sa.earthlink.net>
Content-Type: text/plain;	charset="iso-8859-1"

Hi Histonetters!

What's your New Year's Resolution?  

Of course I will try to eat right and exercise.

 

But... My Resolution is to help even more histo techs find the right
opportunity in the right place at the right time.

 In 2007 I helped people make the move into a management role, relocate
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their advanced degrees, get better salaries, shifts and benefits.

 

In 2008 I resolve to help even more people make the changes they want to
make in order to improve their career situations and ultimately their
quality of life.

 

Whatever you want to do Wherever you want to be.  Let Me Help!

 

Take Advantage of RELIA's Career Coaching Program.  

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within 24 hours or less. 
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Here are some of my current openings:

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I would be more than happy to assist you.  You can reach me at
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Thank You!

 

Pam Barker
President
RELIA
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell:     (407)353-5070
FAX:     (407)678-2788
E-mail: relia1 <@t> earthlink.net
<http://home.earthlink.net/~relia1>
www.myspace.com/pamatrelia



------------------------------

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