[Histonet] hISTOcHEMICAL STAIN FOR GANGLION cELLS

[Robert Fauck [CCDHB]]

Hi Everybody,
 
Does somebody can help me with a reliable (fast) stain for Ganglion
cells for Hirsch Sprung Disease?! Our ACHE stain for it is playing up at
the moment, I think it is due to the age of our Tetra isopropyl
pyrophosphoramide ( OMPA ) chemical in the freezer.
 
Is it adequate to use a fresh piece of Appendix for a control?
 
Thanking you in advance for your help.
 
Regards,
 
Robert Fauck
Wellington Hospital
New Zealand
 
 



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