[Histonet] BrdU/DCX problem
olek.michalski <@t> nencki.gov.pl
Mon Jan 14 00:39:16 CST 2008
2008-01-10 19:11:20 Jonathan Frank <jfrank <@t> med.unc.edu> wrote:
> Thanks for the response. Do you do 1st primary, 1st secondary, then brdU
> primary, and BrdU secondary?
It is exactly the case. If you like I can ask my coleague, who actually
worked out the protocol and uses it routinely for a detailed list of
opertions with some comments. At first glance I see some differences in
BrdU labelling procedure.
> Also, which BrdU primary antibody do you use?
For BrdU we use antibody from Roche. I don't remember the catalogue no.
and the characteristics I can found at the moment is a bit old but I
suppose they offer only one kind.
Laboratory of Neurobiology
of Development and Evolution
Nencki Institute of Experimental Biology
ul. Pasteura 3, 02-093 Warszawa, Poland
Tel. +48 22 5892268, Fax +48 22 8225342
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