[Histonet] lipoma fixation(for paraffin embedding)

[Geoff McAuliffe]

Unbuffered formalin will be acidic but I suggest 10% formalin buffered 
with 2% calcuim acetate. The calcium improves preservation of lipids.

Geoff

FU,DONGTAO wrote:
> Hi, all
>
>  We will harvest lipomas and was asked to fix them in acid formalin. 
> Does anyone have experiences on it? How to make acid formalin? or 
> where to buy it? How long should I fix them? If I use 10%NBF instead 
> of acid formalin, how long should I fix them?
>
>  Many thanks,
>
> Ann Dongtao Fu MD, Ph.D
> Lab Manager
> Dept. of Pathology
> Lab phone: 352-273-7752
> Lab FAX: 352-273-7755
> Lab address: D11-50
> PO Box: 100275
> 1600 SW Archer Road
> University of Flodrida
> Gainesville, FL 32610
>
>
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> Histonet <@t> lists.utsouthwestern.edu
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>
>


-- 
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff <@t> umdnj.edu
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