[Histonet] Paneth cell dyes or markers

[Geoff McAuliffe]

PAS works well after buffered formalin or Bouin fixation.
If you want to get cute, follow the PAS with Alcian blue, pH 2.5. The 
rim of most of the granules will be blue.
These stains are not specific for Paneth cells, but they will 
demonstrate them very nicely.
An immuno stain for lysozyme should also work.

Geoff

Yu, Jian wrote:
> Does anyone know a good Paneth cell specific dye or IHC marker that
> works on paraffin sections of mouse small intestine?
>
> Thanks a lot!
>
> Jian Yu, Ph.D.
> University of Pittsburgh Cancer Institute
> Hillman Cancer Center Research Pavilion
> Office Suite 2.26h, Laboratory 2.43
> 5117 Centre Avenue, Pittsburgh, PA 15213
>
> Phone: 412-623-7786, (Lab) 412-623-3255
> Fax:     412-623-7778
> Email: yuj2 <@t> upmc.edu
>
>
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff <@t> umdnj.edu
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