AW: [Histonet] Mart-1/Melan-A as a stain for melanoma sentinel nodes

Gudrun Lang gu.lang <@t>
Thu Jan 10 14:24:24 CST 2008

We use the Panmelanoma-Cocktail from Biocare.
This links shows the information of Nordiqc about this epitope.

Gudrun Lang
Biomed. Analytikerin
Akh Linz
Krankenhausstr. 9
4020 Linz

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t>
[mailto:histonet-bounces <@t>] Im Auftrag von Barnhart,
Gesendet: Donnerstag, 10. Jänner 2008 17:51
An: Histonet (E-mail)
Betreff: [Histonet] Mart-1/Melan-A as a stain for melanoma sentinel nodes

We are having some debate about which marker to use for our melanoma
sentinel node screens.  We have been using Mart-1, but recently had a case
where we think mast cells were stained.  We can find no literature that
indicates that Mart-1 will stain mast cells.  We use Lab Vision's
Mart-1/Melan-a AB-3 (clone M27C10+M2-9e3).  We ran a Mast Cell tryptase to
see what stained, and all the cells that stained with the Mart-1 stained
plus a bunch of others.  So if the Mart-1 is staining mast cells, it is only
staining a subset of them.  We really think that these are mast cells and
not melanoma, but would like to see some literature to support this.  So two
questions: 1) What are people using as a screening antibody and 2) Does
anyone have a reference that indicates that this antibody will stain mast
cells.  Thanks in advance for your help.

Tammy Barnhart, BS, HTL(ASCP)
Anatomic Pathology Supervisor
St. Alexius Medical Center
Bismarck, ND

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