[Histonet] Lactating Mammary gland fixation

[Jo-Ann Bader, Ms.]

We  are proessing whole, lactating mouse mammary glands  and some times the nuclei just doesn't look right, the look blurry or smeared. I am presuming it is fixation but if anyone else has more experience with lactating mammary glands I am all ears.  We usually fix our tissue in 10% NBF for 24 hours at room temperature.  I am planning to increase out fixation time and add agitation.  If someone has other ideas or a different fixative I would be very interested  in knowing.

Thank You

Jo-Ann Bader
Histology Facility Coordinator
Molecular Oncology Group
MUHC/RVH
687 Pine Ave. W - Rm. M11-53
Montreal, QC, H3A-1A1
Tel: 514-934-1934 Ext: 31780
Fax: 514-843-1479
email: jo-ann.bader <@t> mcgill.ca