[Histonet] Lactating Mammary gland fixation
Jo-Ann Bader, Ms.
jo-ann.bader <@t> mcgill.ca
Thu Jan 10 12:49:13 CST 2008
We are proessing whole, lactating mouse mammary glands and some times the nuclei just doesn't look right, the look blurry or smeared. I am presuming it is fixation but if anyone else has more experience with lactating mammary glands I am all ears. We usually fix our tissue in 10% NBF for 24 hours at room temperature. I am planning to increase out fixation time and add agitation. If someone has other ideas or a different fixative I would be very interested in knowing.
Histology Facility Coordinator
Molecular Oncology Group
687 Pine Ave. W - Rm. M11-53
Montreal, QC, H3A-1A1
Tel: 514-934-1934 Ext: 31780
email: jo-ann.bader <@t> mcgill.ca
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