[Histonet] c-kit (CD117) Ab & Protocol needed - 4%PFA Frozen Mouse Testes Sections

[djemge <@t> aol.com]

Hello Histonetters,
Please share a?working c-kit ab (company, cat. #, dilution) and detailed protocol used in your lab for 4%PFA,?30% sucrose cryoprotected, Frozen Mouse Testes sections.?We have been using an ab by Santa Cruz and haven't had much success on this tissue. I have tried our Ab at different dilutions, with 0.3% H202 quenching, with and w/out sodium citrate HEIR, blocked with goat serum. So far no luck or lots of background. Help!

Donna 

Donna Emge, HT-ASCP
Northwestern University
303 E. Superior, Lurie 7-220
Chicago, IL 60611
312-503-2036
d-emge <@t> northwestern.edu

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