[Histonet] RE: CJD procedures
Meilus, Sheri D.
Sheri.Meilus <@t> va.gov
Mon Jan 7 09:08:23 CST 2008
I have found the National Prion Disease Pathology Surveillance Center to be very helpful. Their web address is www.cjdsurveillance.com
S
Sheri Meilus, HT(ASCP)QIHC
Anatomic Pathology Supervisor
Bay Pines VAMC Bay Pines, FL 33744
727-398-6661 4596
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Saturday, January 05, 2008 1:02 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 50, Issue 6
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Today's Topics:
1. RE: Histonet Digest, Vol 50, Issue 5 (Cook, Sharon )
2. Re: RE: HT and HTL "Salary Survey" posting (Kim Merriam)
3. PEEK and PLLA/PGA (Nancy W. Troiano)
4. Ki-67 (Kalleberg, Kristopher)
5. RE: Pressure cooker question (Jo Dee Fish)
6. CJD Histology Procedures (Dawson, Glen)
7. Re: Re: Lendrum stains (Bryan Llewellyn)
8. histochemistry an extinct art? (Gudrun Lang)
9. RE: Re: Lendrum stains (Houston, Ronald)
10. Course in Histology (Shanu Mehta)
11. Sausage blocks (Webb, Dorothy L)
12. Surfactant A protein (Beth Delescavage)
13. Re: CJD Histology Procedures (Amy Porter)
14. Re: Course in Histology (Godfrey Guerzon)
15. jackson immuno backorder (Emily Sours)
16. Re: histochemistry an extinct art? (Bryan Llewellyn)
17. Re: histochemistry an extinct art? (Rene J Buesa)
18. on call coverage (Carrie Disbrow)
19. RE: on call coverage (Weems, Joyce)
20. Re: immunocal (Maxim Peshkov)
21. RE: immunocal (Weems, Joyce)
22. Re: on call coverage (Victoria Baker)
----------------------------------------------------------------------
Message: 1
Date: Fri, 4 Jan 2008 13:04:37 -0500
From: "Cook, Sharon " <Sharon.Cook <@t> osumc.edu>
Subject: [Histonet] RE: Histonet Digest, Vol 50, Issue 5
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<7A541592F9A53A4E86E7A3D5C4C7F68C01D7962B <@t> msxc01.osumc.edu>
Content-Type: text/plain; charset="us-ascii"
I've unsubscribed 2 times and I continue to receive these emails. Please
unsubscribe for me.
Thanks,
Sharon S. Cook, MT(ASCP) SBB
Operations Director, Anatomic Pathology
The Ohio State University
E407 Doan Hall
410 West 10th Avenue
Columbus, OH 43210
sharon.cook <@t> osumc.edu
Phone: 614 / 293-8418
Fax: 614 / 293-2779
Pager: 614 / 346-0746
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
histonet-request <@t> lists.utsouthwestern.edu
Sent: Friday, January 04, 2008 1:05 PM
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Subject: Histonet Digest, Vol 50, Issue 5
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Contents of Histonet digest..."
Today's Topics:
1. Re: Lendrum stains (Robert Richmond)
----------------------------------------------------------------------
Message: 1
Date: Fri, 4 Jan 2008 12:50:03 -0500
From: "Robert Richmond" <RSRICHMOND <@t> aol.com>
Subject: [Histonet] Re: Lendrum stains
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<abea52a60801040950x62eec247k977ca0a5fb9ab6b1 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Sharon E. Willman (where?) asks for information about "Lendrun" and Fraser
stains, she doesn't say for what.
A.C. Lendrum, I was told back around 1970, was one of the last histologists
who understood textile dyes. Apparently there were a lot of small factories
making obscure dyes in Scotland back then, and he knew all of these single
malt dye-makers and collected numerous samples. His eclectic habits make it
difficult to identify the dyes he used, let alone obtain and use them. That
and the invention of immunohistochemistry probably consign most of his work
to oblivion.
Lendrum's "Obadiah" stain (he was fond of such fanciful names) for fibrin was
in use in a research lab at Cornell Medical Center on east 68th street in New
York City when I was there briefly in 1968.
Stainsfile gives the following reference: Lendrum, A. C., et. al.
(1962) "Studies on the character and staining of fibrin." Journal of Clinical
Pathology, v. 15, p. 401. [this is a British journal, not the AJCP]. Rotsa
ruck finding naphthalene blue black CS, Chicago red, or polar brilliant red
BN.
John Kiernan - Dick Dapson - Mike Titford - other geezers on this list
- do you know anything more about A.C. Lendrum? He must have been an
interesting guy!
Bob Richmond
Samurai Pathologist
Knoxville TN
------------------------------
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End of Histonet Digest, Vol 50, Issue 5
***************************************
------------------------------
Message: 2
Date: Fri, 4 Jan 2008 10:21:23 -0800 (PST)
From: Kim Merriam <kmerriam2003 <@t> yahoo.com>
Subject: Re: [Histonet] RE: HT and HTL "Salary Survey" posting
To: relia1 <@t> earthlink.net, Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <564776.10450.qm <@t> web50306.mail.re2.yahoo.com>
Content-Type: text/plain; charset=us-ascii
Thanks for the tip Pam. I have actually had #3 happen to me (about 10 years ago or so):
I had been at my new job for about 3 months and I got a call from someone in HR telling me that she had just received my resume from a recruiter. I had to explain to her that, obviously, I had been looking for a position prior to my arrival at that company so it must have been a left-over, but it was nevertheless an embarrassing moment for me (she was less than understanding - "human" relations was not her strong point; not so good for someone working in HR). I called the recruiter and gave him a piece of my mind!! He should not have sent my resume to anyone without my permission.
Kim Merriam, MA, HT(ASCP)
Cambridge, MA
----- Original Message ----
From: Pam Barker <relia1 <@t> earthlink.net>
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, January 3, 2008 4:43:08 PM
Subject: [Histonet] RE: HT and HTL "Salary Survey" posting
Hi Histonetters!
I hope everybody had a wonderful holiday season. I just read the
posting from Robert Garhart a recruiter at System One Search.
I just wanted to comment to those of you who might not be aware that by
releasing this information you are actually releasing basic pre
interview information. I am not saying don't do it I am just saying be
aware of how the information could potentially be used. (i.e. Your
information could be released to an employer for a potential job opening
without your permission). By releasing this information you could be
giving up your confidentiality. Having been a professional recruiter
for over 25 years I have seen these types of requests before and more
importantly the havoc it can wreak for an individual. Here are several
scenarios I have seen:
Each of these scenarios starts this way: The recruiter takes the
information you have supplied for this "survey" and gives it to a client
(a fishing expedition of sorts), without your knowledge or permission,
then once there is interest from the client they contact you, regardless
of whether or not you are looking for a job.
Here are the potential outcomes:
1. You send your resume to a client and are not considered for the
position because you have already been submitted by a recruiter without
your knowledge or permission and the client is not paying fees at that
time.
2. You are working with a recruiter who you have consented to have
represent you at a client and that recruiter who you trust to
represent you has accurately given you all the information on the
position before submitting your resume and has done all of the footwork
with the client is excluded from your placement because you have been
submitted by another recruiter without your knowledge or permission. In
this scenario you could also be cut from consideration because employers
don't like to get caught up in conflicts between recruiters. Not to
mention it makes you the candidate, look unprofessional by not realizing
that someone you have not given permission to is representing you.
3. Your resume is sent to your current employer or an affiliate
facility without your permission or knowledge - OUCH!!.
4. By giving this information you could be in violation of your
company's own confidentialty policy.
This information could also be sold for SPAM or used to build a
candidate database.
And as most of you know the information he is looking for is actually
available in a confidential format free of charge at both the NSH and
ASCP websites and at the website Salary.com
I am not saying don't respond I am merely saying be aware of what could
potentially result from responding.
In this day and age it is very important to have control of your
personal information, who you give it out to and how it is used.
Thank You!
Pam Barker
President
RELIA
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: relia1 <@t> earthlink.net
<http://home.earthlink.net/~relia1>
www.myspace.com/pamatrelia
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know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ
------------------------------
Message: 3
Date: Fri, 04 Jan 2008 13:22:59 -0500
From: "Nancy W. Troiano" <nancy.troiano <@t> yale.edu>
Subject: [Histonet] PEEK and PLLA/PGA
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <5.2.1.1.2.20080104132124.024477f0 <@t> email.med.yale.edu>
Content-Type: text/plain; format=flowed; charset=us-ascii
Hi Joanne - we have cut blood vessels containing PLLA and PGA implants and
use glycolmethacrylate since the PLLA and PGA can tear through
paraffin. You may want to try GMA - we use the Technovit 7100 kit.
------------------------------
Message: 4
Date: Fri, 4 Jan 2008 13:25:58 -0500
From: "Kalleberg, Kristopher" <Kristopher.Kalleberg <@t> unilever.com>
Subject: [Histonet] Ki-67
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<0E6BC087F70F9C47ACFF2C203D6E329C0376DE99 <@t> NTRSEVS30002.s3.ms.unilever.com>
Content-Type: text/plain; charset="us-ascii"
Hello All,
I know most people have worked with the biomarker Ki-67. The question
that has come about is why a few nuclei are staining in suprabasal
layers of skin and not just specifically in the basal layer where they
should be specifically assigned to. I am investigating both
photoprotected sites of the arm along with solar lentigines (sun
spots/freckles) of the arm. If anyone can help me answer this, I would
greatly appreciate it. Thank you.
Kris Kalleberg
------------------------------
Message: 5
Date: Fri, 4 Jan 2008 10:34:10 -0800
From: "Jo Dee Fish" <jfish <@t> gladstone.ucsf.edu>
Subject: RE: [Histonet] Pressure cooker question
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000001c84f00$65c7db10$2e0d010a <@t> JFISH>
Content-Type: text/plain; charset="us-ascii"
Hello everyone,
I just want to thank all of those who responded to my pressure cooker
question. I have decided to order a "3-in-1" cooker from Sears. It is able
to be more flexible, it can either steam or pressure cook, as well as slow
cook (which we won't be doing unless we make BBQ meatballs to go with our
beer on Fridays!), so we can try different temperatures and times to
optimize our protocol. I understand that the "scientific" pressure cookers
are probably more accurate as far as temperature is concerned, but the price
is prohibitive which is why I decided to buy the less expensive household
version.
Thanks for all of your help and advice,
Jo Dee
~~Jo Dee Fish~~
Research Technologist III
Gladstone Institute of Cardiovascular Disease
Telephone: (415) 734-2567
Fax: (415) 355-0824
E-mail: jfish <@t> gladstone.ucsf.edu
Mailing address:
The J. David Gladstone Institutes
1650 Owens Street
San Francisco, CA 94158
-----Original Message-----
From: Tom McNemar [mailto:TMcNemar <@t> lmhealth.org]
Sent: Friday, January 04, 2008 6:28 AM
To: Bernice Frederick; jfish <@t> gladstone.ucsf.edu;
histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Pressure cooker question
Interesting. I used the Biocare decloaker for 5 minutes and charged slides.
My tissues came off at least 50% of the time. Do you let your slides dry
overnight before running them?
Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org
-----Original Message-----
From: Bernice Frederick [mailto:b-frederick <@t> northwestern.edu]
Sent: Friday, January 04, 2008 8:54 AM
To: Tom McNemar; jfish <@t> gladstone.ucsf.edu; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Pressure cooker question
We use the Biocare decloaker for antigen retrieval. It is a pressure cooker
and specifically programmed. Our sections stay on. We like to cook the
slides overnight.
Bernice
Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tom McNemar
Sent: Friday, January 04, 2008 6:43 AM
To: jfish <@t> gladstone.ucsf.edu; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Pressure cooker question
I used to use a pressure cooker but found it to be very harsh. I had a lot
of trouble getting the sections to stay on the slide. For the last several
years I'v use a $40 Black and Decker rice steamer. Slower than the pressure
cooker but it always works and no problems with the sections. You might
also look into microwave retrieval. I tried it a few times and it worked
fine.
I steam them for 20 minutes with a 20 minute cooling afterward. That gives
me plenty of time to make up my buffer and set up my run.
Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Jo Dee Fish
Sent: Thursday, January 03, 2008 3:26 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Pressure cooker question
We are searching for a cheap but reliable way to do antigen retrieval. Is
anyone out there using an electric pressure cooker? And, are the ordinary
household appliances working for you?
Thanks in advance for your help.
Jo Dee
~~Jo Dee Fish~~
Research Technologist III
Gladstone Institute of Cardiovascular Disease
Telephone: (415) 734-2567
Fax: (415) 355-0824
E-mail: <mailto:jfish <@t> gladstone.ucsf.edu> jfish <@t> gladstone.ucsf.edu
Mailing address:
The J. David Gladstone Institutes
1650 Owens Street
San Francisco, CA 94158
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
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------------------------------
Message: 6
Date: Fri, 4 Jan 2008 12:41:38 -0600
From: "Dawson, Glen" <GDawson <@t> dynacaremilwaukee.com>
Subject: [Histonet] CJD Histology Procedures
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<B3D65550856D0146B900D401EE313D4B01273BDC <@t> dynams.dynacaremilwaukee.com>
Content-Type: text/plain; charset="iso-8859-1"
All,
Could someone point me in the right direction for the most up-to-date CJD Procedures for the Histolab. Ours may be out of date and I need something to compare them too.
Thanx In Advance,
Glen Dawson BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI
------------------------------
Message: 7
Date: Fri, 04 Jan 2008 10:58:28 -0800
From: Bryan Llewellyn <llewllew <@t> shaw.ca>
Subject: Re: [Histonet] Re: Lendrum stains
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000801c84f03$cb9ba310$05024246 <@t> yourlk4rlmsu>
Content-Type: text/plain; format=flowed; charset=iso-8859-1;
reply-type=original
I trained in the UK during the early 1960s and went to Bromley Technical
College every Friday night, a real pain since it was on the edge of South
London and I lived on the edge of North London. The instructor was
Wallington, of the text book, and he was regularly in contact with Lendrum.
Even in those days (63 and 64) Lendrum was an exception among "dye
tinkerers". Wallington told us that producing new methods for fibrin was
pretty much a hobby for Lendrum. The one noted as the long method on
StainsFile is actually from a variation given to Wallington by Lendrum
earlier in that week of that Friday's lecture.
I used to use all those methods when I was in Winnipeg during the 1970s,
mostly on rejecting kidney transplants. Even then immunifluorescence was
replacing staining methods for fibrin, certainly on renal biopsies. I
always found the Obadiah an unsatisfactory stain, the Masson 44/41 too. The
colours just didn't look right to me. The picro-mallory is, however, one of
the stars in trichrome staining if done on properly prepared material. I
always found it absolutely beautiful to look at, but then, I am noted as
being a bit strange about things like that.
I have substituted amido black 10B (naphthol blue black, CI # 20470) for
naphthalene blue black CS, and it worked fine. They are structurally very
similar.
Bryan Llewellyn
----- Original Message -----
From: "Robert Richmond" <RSRICHMOND <@t> aol.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, January 04, 2008 9:50 AM
Subject: [Histonet] Re: Lendrum stains
> Sharon E. Willman (where?) asks for information about "Lendrun" and
> Fraser stains, she doesn't say for what.
>
> A.C. Lendrum, I was told back around 1970, was one of the last
> histologists who understood textile dyes. Apparently there were a lot
> of small factories making obscure dyes in Scotland back then, and he
> knew all of these single malt dye-makers and collected numerous
> samples. His eclectic habits make it difficult to identify the dyes he
> used, let alone obtain and use them. That and the invention of
> immunohistochemistry probably consign most of his work to oblivion.
>
> Lendrum's "Obadiah" stain (he was fond of such fanciful names) for
> fibrin was in use in a research lab at Cornell Medical Center on east
> 68th street in New York City when I was there briefly in 1968.
> Stainsfile gives the following reference: Lendrum, A. C., et. al.
> (1962) "Studies on the character and staining of fibrin." Journal of
> Clinical Pathology, v. 15, p. 401. [this is a British journal, not the
> AJCP]. Rotsa ruck finding naphthalene blue black CS, Chicago red, or
> polar brilliant red BN.
>
> John Kiernan - Dick Dapson - Mike Titford - other geezers on this list
> - do you know anything more about A.C. Lendrum? He must have been an
> interesting guy!
>
> Bob Richmond
> Samurai Pathologist
> Knoxville TN
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 8
Date: Fri, 4 Jan 2008 20:26:11 +0100
From: "Gudrun Lang" <gu.lang <@t> gmx.at>
Subject: [Histonet] histochemistry an extinct art?
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000501c84f07$aad39ee0$eeeea8c0 <@t> dielangs.at>
Content-Type: text/plain; charset="us-ascii"
Hi,
I have a question for those, that are experienced with the old and the new
techniques in histotechnology. I looked through some antiquariat-books, that
deal a lot with histochemical demonstration of many tissue-compounds and
enzymes. These techniques sound all very strange to me and it seems like a
forgotten art.
I work in a clinical histolab. Are the histochemical, enzymhistochemical
methods still in use in research-labs? Did immunhistochemistry replace them
all together? Is histochemistry a part of modern cellbiology or just
obsolete?
Gudrun Lang
------------------------------
Message: 9
Date: Fri, 4 Jan 2008 14:25:56 -0500
From: "Houston, Ronald" <Ronald.Houston <@t> nationwidechildrens.org>
Subject: RE: [Histonet] Re: Lendrum stains
To: "Robert Richmond" <RSRICHMOND <@t> aol.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<979FF5962E234F45B06CF0DB7C1AABB2145D9FC8 <@t> chi2k3ms01.columbuschildrens.net>
Content-Type: text/plain; charset="us-ascii"
I fondly remember performing Lendrum's stains in a previous life back
home in Scotland. The color contrasts of many of the techniques remains
unsurpassed as far as tinctorial staining is concerned.
Not so sure why none of the methods really took off in the States;
perhaps you're right, Bob, it may have had to do with the fact that he
got many of the dyes directly from the mills.
Professor Alan C Lendrum was Professor of Pathology at both Glasgow and
Dundee Universities (the latter until 1972 when I believe he retired).
Perhaps John Bancroft, Peter Stoward and/or Richard Horobin would be
able to comment more on Professor Lendrum, if they still subscribe to
Histonet. It is certainly true that he was one of the last
histopathologists who understood the properties of the dyes and the
theory behind the techniques he introduced with his colleagues.
His work was true Art in histology; alas a dieing trait (no pun
intended).
As far as I am concerned, his MSB (Martius Yellow, Crystal Scarlet,
Soluble Blue) is still the best method for fibrin demonstration.
Ronnie
Ronnie Houston, MS, HT(ASCP)QIHC
Anatomic Pathology Manager
Nationwide Children's Hospital
700 Children's Drive
Columbus, OH 43205
(614) 722 5465
Ronald.Houston <@t> NationwideChildrens.org
Columbus Children's Hospital is now Nationwide Children's Hospital
www.NationwideChildrens.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Robert
Richmond
Sent: Friday, January 04, 2008 12:50 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Lendrum stains
Sharon E. Willman (where?) asks for information about "Lendrun" and
Fraser stains, she doesn't say for what.
A.C. Lendrum, I was told back around 1970, was one of the last
histologists who understood textile dyes. Apparently there were a lot
of small factories making obscure dyes in Scotland back then, and he
knew all of these single malt dye-makers and collected numerous
samples. His eclectic habits make it difficult to identify the dyes he
used, let alone obtain and use them. That and the invention of
immunohistochemistry probably consign most of his work to oblivion.
Lendrum's "Obadiah" stain (he was fond of such fanciful names) for
fibrin was in use in a research lab at Cornell Medical Center on east
68th street in New York City when I was there briefly in 1968.
Stainsfile gives the following reference: Lendrum, A. C., et. al.
(1962) "Studies on the character and staining of fibrin." Journal of
Clinical Pathology, v. 15, p. 401. [this is a British journal, not the
AJCP]. Rotsa ruck finding naphthalene blue black CS, Chicago red, or
polar brilliant red BN.
John Kiernan - Dick Dapson - Mike Titford - other geezers on this list
- do you know anything more about A.C. Lendrum? He must have been an
interesting guy!
Bob Richmond
Samurai Pathologist
Knoxville TN
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Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Message: 10
Date: Fri, 4 Jan 2008 14:34:25 -0500
From: "Shanu Mehta" <smehta <@t> magenbiosciences.com>
Subject: [Histonet] Course in Histology
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<4C78CF3C61E6A640888226885709EFE9379DAB <@t> server01.magen.local>
Content-Type: text/plain; charset="US-ASCII"
Hello Histonetters,
Is there a histology course I can take anywhere in the Boston area? I
did not take any formal training/education in Histology but have been
doing the same for almost 2 years now. It would be great if I could get
some formal knowledge in Histology to help me understand the basic
problems I face sometimes during my routine work.
Thanks a lot for any help with this.
Shanu
------------
Shanu Mehta
Magen Biosciences
100 Beaver St.Suite 101
Waltham, MA 02453
Direct Dial: 781-314-2918
Fax: 781-314-2999
Main Line: 781-314-2900
Email: smehta <@t> magenbiosciences.com
http://www.magenbiosciences.com
------------------------------
Message: 11
Date: Fri, 04 Jan 2008 13:46:30 -0600
From: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>
Subject: [Histonet] Sausage blocks
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<0E394B648E5284478A6CCB78E5AFDA2705635392 <@t> hpes1.HealthPartners.int>
Content-Type: text/plain; charset="us-ascii"
Does anyone use sausage blocks for their IHC controls? I have heard
pros and cons to this control usage, the cons being mostly too time
consuming to keep up. Would like some input as to what others are doing
in this regard!! Thanks ahead of time!!
Dorothy Webb, HT (ASCP)
Histology Technical Supervisor
Regions Hospital, Pathology Department
640 Jackson Street, Saint Paul, MN 55101-2595
Phone: 651-254-2962
Fax: 651-254-2741
Regions Hospital is part of the HealthPartners family of care
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Message: 12
Date: Fri, 4 Jan 2008 12:00:22 -0800
From: "Beth Delescavage" <bdelescavage <@t> cellnetix.com>
Subject: [Histonet] Surfactant A protein
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E32734DDF909E2468582F132951FD0E515594B <@t> cel-exc-001.cellnetix.local>
Content-Type: text/plain; charset="us-ascii"
Hi All~
Thermo/Neomarkers/ Lab Vision has discontinued the Surfactant A clone
PE10 antibody we have been using and are not offering any replacements.
Are any of you using the same clone from a different vendor and do you
like the staining?
Have a nice weekend!
~Beth
Beth Delescavage, BS, HTL (ASCP) QIHC
CellNetix Laboratories
Histotechnologist
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This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message.
------------------------------
Message: 13
Date: Fri, 4 Jan 2008 15:47:41 -0500
From: "Amy Porter" <portera <@t> msu.edu>
Subject: Re: [Histonet] CJD Histology Procedures
To: "Dawson, Glen" <GDawson <@t> dynacaremilwaukee.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <002001c84f13$0c9b33d0$8e7a0923 <@t> histolab>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
I would recommend the CDC website.
Amy S. Porter, HT (ASCP) QIHC
Investigative HistoPathology Laboratory - Supervisor
2201 Biomedical Physical Sciences Bldg. Rm #2133
East Lansing, MI 48824-3320
Phone: (517) 355-6475 ext 1480
Fax: (517) 432-1368
Email: portera <@t> msu.edu
Web: www.humanpathology.msu.edu
----- Original Message -----
From: "Dawson, Glen" <GDawson <@t> dynacaremilwaukee.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, January 04, 2008 1:41 PM
Subject: [Histonet] CJD Histology Procedures
All,
Could someone point me in the right direction for the most up-to-date CJD
Procedures for the Histolab. Ours may be out of date and I need something
to compare them too.
Thanx In Advance,
Glen Dawson BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI
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------------------------------
Message: 14
Date: Fri, 04 Jan 2008 16:07:23 -0500
From: "Godfrey Guerzon" <Gguerzon <@t> lifebridgehealth.org>
Subject: Re: [Histonet] Course in Histology
To: <histonet <@t> lists.utsouthwestern.edu>, "Shanu Mehta"
<smehta <@t> magenbiosciences.com>
Message-ID: <s77e59d1.094 <@t> lifebridgehealth.org>
Content-Type: text/plain; charset=US-ASCII
There is a web course in Histotechnology at Harford Community College in
Maryland. You may want to check it out. The Director of the program is
Mr. Floyd Grimm but I believe he is retiring. His phone number is (410)
836-4372. His e-mail address fgrimm <@t> harford.edu. You may also check
the college website.
Godfrey
>>> "Shanu Mehta" <smehta <@t> magenbiosciences.com> 1/4/2008 2:34 PM >>>
Hello Histonetters,
Is there a histology course I can take anywhere in the Boston area? I
did not take any formal training/education in Histology but have been
doing the same for almost 2 years now. It would be great if I could
get
some formal knowledge in Histology to help me understand the basic
problems I face sometimes during my routine work.
Thanks a lot for any help with this.
Shanu
------------
Shanu Mehta
Magen Biosciences
100 Beaver St.Suite 101
Waltham, MA 02453
Direct Dial: 781-314-2918
Fax: 781-314-2999
Main Line: 781-314-2900
Email: smehta <@t> magenbiosciences.com
http://www.magenbiosciences.com
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Message: 15
Date: Fri, 4 Jan 2008 16:19:00 -0500
From: "Emily Sours" <talulahgosh <@t> gmail.com>
Subject: [Histonet] jackson immuno backorder
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<b39794b0801041319y10e5e8fcmb9abe41ffc3a570b <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Has anyone had Jackson Immunoresearch say their secondary antibodies
are on backorder indefinitely?
Another lab was told it would be an indefinite amount of time before
they could get them.
Emily
--
"Prosperity ripened the principle of decay...and, as soon as time or
accident and removed the artificial supports, the stupendous fabric
yielded to the pressure of its own weight...instead of inquiring why
the Roman Empire was destroyed we should rather be surprised that it
has subsisted for so long"
-Edward Gibbon, Decline and Fall of the Roman Empire
------------------------------
Message: 16
Date: Fri, 04 Jan 2008 12:57:11 -0800
From: Bryan Llewellyn <llewllew <@t> shaw.ca>
Subject: Re: [Histonet] histochemistry an extinct art?
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <002801c84f14$604e65f0$05024246 <@t> yourlk4rlmsu>
Content-Type: text/plain; format=flowed; charset=iso-8859-1;
reply-type=original
Immunohistochemistry is certainly the latest technique to coma along, and is
undoubtedly of very great value. It is now in its infancy, I suspect, and I
fully expect that IHC procedures will increase in usefulness as time passes.
Probably some antibodies will stop being used as clinical correlation with
specific antobodies becomes more standardised.
However, the commonest method used in histolabs today is about 120 years
old, or more. That is the H&E. There is no sign that it is going into
"that long night". Much the same can be said for Masson's trichrome and its
clones, the PAS histochemical procedure, Perls' histochemical procedure.
How do you demonstrate reticulin, or elastic? Enzyme histochemistry is
still used to demonstrate muscle fibre types. The list could go on, but I
am of the opinion that the older methods still have great value and will
continue to have great value for a long time to come. Sometimes they are
far more convenient than IHC (toluidine blue for helicobacter, for
instance), even when IHC is pushed for it.
Part of the reason is that IHC and enzyme histochemistry, for instance,
demonstrate different things. If an antibody against alkaline phosphatase
is used, you will demonstrate the enzyme, i.e. the protein. If you do an
alkaline phosphatase stain using naphthol phosphate and a diazonium salt,
you demonstrate the activity of the enzyme. Those are two different things.
I know I am a bit of an old fogey on this, but I always strongly recommend
that histotechs become conversant with the whole range of procedures used
histologically, dye staining, histochemistry and IHC.
Bryan Llewellyn
----- Original Message -----
From: "Gudrun Lang" <gu.lang <@t> gmx.at>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, January 04, 2008 11:26 AM
Subject: [Histonet] histochemistry an extinct art?
> Hi,
>
> I have a question for those, that are experienced with the old and the new
> techniques in histotechnology. I looked through some antiquariat-books,
> that
> deal a lot with histochemical demonstration of many tissue-compounds and
> enzymes. These techniques sound all very strange to me and it seems like a
> forgotten art.
>
> I work in a clinical histolab. Are the histochemical, enzymhistochemical
> methods still in use in research-labs? Did immunhistochemistry replace
> them
> all together? Is histochemistry a part of modern cellbiology or just
> obsolete?
>
>
>
> Gudrun Lang
>
>
--------------------------------------------------------------------------------
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 17
Date: Fri, 4 Jan 2008 13:26:13 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] histochemistry an extinct art?
To: Bryan Llewellyn <llewllew <@t> shaw.ca>, Histonet
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <430699.41709.qm <@t> web61220.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Amen to all that from the bottom of my very old heart!
René
Bryan Llewellyn <llewllew <@t> shaw.ca> wrote: Immunohistochemistry is certainly the latest technique to coma along, and is
undoubtedly of very great value. It is now in its infancy, I suspect, and I
fully expect that IHC procedures will increase in usefulness as time passes.
Probably some antibodies will stop being used as clinical correlation with
specific antobodies becomes more standardised.
However, the commonest method used in histolabs today is about 120 years
old, or more. That is the H&E. There is no sign that it is going into
"that long night". Much the same can be said for Masson's trichrome and its
clones, the PAS histochemical procedure, Perls' histochemical procedure.
How do you demonstrate reticulin, or elastic? Enzyme histochemistry is
still used to demonstrate muscle fibre types. The list could go on, but I
am of the opinion that the older methods still have great value and will
continue to have great value for a long time to come. Sometimes they are
far more convenient than IHC (toluidine blue for helicobacter, for
instance), even when IHC is pushed for it.
Part of the reason is that IHC and enzyme histochemistry, for instance,
demonstrate different things. If an antibody against alkaline phosphatase
is used, you will demonstrate the enzyme, i.e. the protein. If you do an
alkaline phosphatase stain using naphthol phosphate and a diazonium salt,
you demonstrate the activity of the enzyme. Those are two different things.
I know I am a bit of an old fogey on this, but I always strongly recommend
that histotechs become conversant with the whole range of procedures used
histologically, dye staining, histochemistry and IHC.
Bryan Llewellyn
----- Original Message -----
From: "Gudrun Lang"
To:
Sent: Friday, January 04, 2008 11:26 AM
Subject: [Histonet] histochemistry an extinct art?
> Hi,
>
> I have a question for those, that are experienced with the old and the new
> techniques in histotechnology. I looked through some antiquariat-books,
> that
> deal a lot with histochemical demonstration of many tissue-compounds and
> enzymes. These techniques sound all very strange to me and it seems like a
> forgotten art.
>
> I work in a clinical histolab. Are the histochemical, enzymhistochemical
> methods still in use in research-labs? Did immunhistochemistry replace
> them
> all together? Is histochemistry a part of modern cellbiology or just
> obsolete?
>
>
>
> Gudrun Lang
>
>
--------------------------------------------------------------------------------
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
_______________________________________________
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------------------------------
Message: 18
Date: Fri, 04 Jan 2008 21:35:58 -0500
From: "Carrie Disbrow" <disbrc <@t> shands.ufl.edu>
Subject: [Histonet] on call coverage
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <477EA6BE.72AC.0059.0 <@t> shands.ufl.edu>
Content-Type: text/plain; charset=US-ASCII
We think our hourly pay to carry the beeper when on call is too low and we have talked to management. We supply coverage on weekends and holidays and we rotate the beeper weekly.
Our employer said if we took a survey and the results revealed our on-call pay is below average we might get an increase.
Would anyone like to participate in our survey?
------------------------------
Message: 19
Date: Sat, 5 Jan 2008 08:39:14 -0500
From: "Weems, Joyce" <JWEEMS <@t> sjha.org>
Subject: RE: [Histonet] on call coverage
To: "Carrie Disbrow" <disbrc <@t> shands.ufl.edu>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1CD6831EB9B26D45B0A3EAA79F7EBD3204726B4B <@t> sjhaexc02.sjha.org>
Content-Type: text/plain; charset="iso-8859-1"
Ours is 3.50/hr - Sundays and holidays. If called back, we have a "call back" clock code, which allows extra for travel. Good luck! j
Joyce Weems
Pathology Manager
Saint Joseph's Hospital of Atlanta
404-851-7376
404-851-7831 - Fax
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Carrie Disbrow
Sent: Fri 1/4/2008 9:35 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] on call coverage
We think our hourly pay to carry the beeper when on call is too low and we have talked to management. We supply coverage on weekends and holidays and we rotate the beeper weekly.
Our employer said if we took a survey and the results revealed our on-call pay is below average we might get an increase.
Would anyone like to participate in our survey?
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 20
Date: Sat, 5 Jan 2008 16:43:38 +0300
From: Maxim Peshkov <Maxim_71 <@t> mail.ru>
Subject: Re: [Histonet] immunocal
To: Gayle Callis <gayle.callis <@t> bresnan.net>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <1761665308.20080105164338 <@t> mail.ru>
Content-Type: text/plain; charset=us-ascii
Gayle:
Does this solution (4% hydrochloric acid/8% formic
acid) damage to IHC in any ways or better use 15%
formic acid for this purpose? We will do IHC for
breast (ER, PgR, Ki67 and Her2).
Maxim Peshkov
Taganrog,
Russia.
------------------------------
Message: 21
Date: Sat, 5 Jan 2008 11:30:44 -0500
From: "Weems, Joyce" <JWEEMS <@t> sjha.org>
Subject: RE: [Histonet] immunocal
To: "Maxim Peshkov" <Maxim_71 <@t> mail.ru>, "Gayle Callis"
<gayle.callis <@t> bresnan.net>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<1CD6831EB9B26D45B0A3EAA79F7EBD3204726B4C <@t> sjhaexc02.sjha.org>
Content-Type: text/plain; charset="iso-8859-1"
Maxim, If Gayle's answer is that your solution does not work well for IHC, there is a product from BBC Biochemical that is designed for IHC - and it does work well. We use it for our bonemarrows.
http://www.bbcus.com
6086 RapidCal*Immuno(tm), 1Pint
6087 RapidCal*Immuno(tm), 1 Quart
6089 RapidCal*Immuno(tm), 1 Gallon
6090 RapidCal*Immuno(tm), 1 Gallon Cube
Cheers! j
Joyce Weems
Pathology Manager
Saint Joseph's Hospital of Atlanta
404-851-7376
404-851-7831 - Fax
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Maxim Peshkov
Sent: Sat 1/5/2008 8:43 AM
To: Gayle Callis
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] immunocal
Gayle:
Does this solution (4% hydrochloric acid/8% formic
acid) damage to IHC in any ways or better use 15%
formic acid for this purpose? We will do IHC for
breast (ER, PgR, Ki67 and Her2).
Maxim Peshkov
Taganrog,
Russia.
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------------------------------
Message: 22
Date: Sat, 5 Jan 2008 12:28:41 -0500
From: "Victoria Baker" <bakevictoria <@t> gmail.com>
Subject: Re: [Histonet] on call coverage
To: "Carrie Disbrow" <disbrc <@t> shands.ufl.edu>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<4f016b690801050928ke0a74acncb4a9f6744cca8e6 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Carrie,
I've worked in different situations where on call was required and
each handled it a bit differently.
In one place we rotated on call/weekend service where we had a day off
during the week, worked a FULL Saturday and took call for the next
day. Our base was $25 for just carrying the beeper on Sunday a
percentage for after hours coverage on Saturday. If we were called
back in then we received automatic 2 hours OT, whether we were there
for 15 minutes or the full 2 hours, any additional hours were covered
at the time and a half rate. If it was a holiday and we were called
back we were compensated at the holiday rate under the same
guidelines.
For weeknight coverage we were paid something like $2 - 3 per hour
with the same OT as weekends if we were called back. This was between
1996-1999.
In another position I was the lab manager and I was essentially on
call 24/7 without any compensation - this meant if I was called late
at night for a freezer alarm or an off hour tissue collection/animal
take down, I had to come in. I was then given "compensatory time off"
in lieu of $'s. In someways this worked out pretty well as I'm a
single parent I used to use this time if I had to take off for a
family reasons.
In another lab, my staff was only on call for weekend/holiday
emergency biopsy services - no frozens were done in our lab - and we
usually knew on Friday night if we would have a case coming through
that would require someone to come in. So coverage would begin on
Saturday morning at 8am and end at 6pm Sunday night. Each tech would
be paid 10% of their hourly rate for just carrying the beeper. If
they were working they would get time and a half for the actual hours
worked and also paid the on call as well. If called in on a holiday
they recieved premium pay which is 2X their hourly wage. The average
on call for 34 hours was $85 which I think was pretty fair and better
than most places I'd seen given that when you take on call your
weekends are pretty limited as to what you can do.
Good luck!
On 1/4/08, Carrie Disbrow <disbrc <@t> shands.ufl.edu> wrote:
> We think our hourly pay to carry the beeper when on call is too low and we have talked to management. We supply coverage on weekends and holidays and we rotate the beeper weekly.
> Our employer said if we took a survey and the results revealed our on-call pay is below average we might get an increase.
> Would anyone like to participate in our survey?
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
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