[Histonet] Pressure cooker question

[Jo Dee Fish]

Hello everyone,
I just want to thank all of those who responded to my pressure cooker
question.  I have decided to order a "3-in-1" cooker from Sears.  It is able
to be more flexible, it can either steam or pressure cook, as well as slow
cook (which we won't be doing unless we make BBQ meatballs to go with our
beer on Fridays!), so we can try different temperatures and times to
optimize our protocol.  I understand that the "scientific" pressure cookers
are probably more accurate as far as temperature is concerned, but the price
is prohibitive which is why I decided to buy the less expensive household
version.
Thanks for all of your help and advice,
Jo Dee 


~~Jo Dee Fish~~
Research Technologist III
Gladstone Institute of Cardiovascular Disease

Telephone: (415) 734-2567
Fax: (415) 355-0824
E-mail: jfish <@t> gladstone.ucsf.edu

Mailing address:  
The J. David Gladstone Institutes
1650 Owens Street
San Francisco, CA 94158

-----Original Message-----
From: Tom McNemar [mailto:TMcNemar <@t> lmhealth.org] 
Sent: Friday, January 04, 2008 6:28 AM
To: Bernice Frederick; jfish <@t> gladstone.ucsf.edu;
histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Pressure cooker question

Interesting.  I used the Biocare decloaker for 5 minutes and charged slides.
My tissues came off at least 50% of the time.  Do you let your slides dry
overnight before running them?

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org


-----Original Message-----
From: Bernice Frederick [mailto:b-frederick <@t> northwestern.edu]
Sent: Friday, January 04, 2008 8:54 AM
To: Tom McNemar; jfish <@t> gladstone.ucsf.edu; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Pressure cooker question


We use the Biocare decloaker for antigen retrieval. It is a pressure cooker
and specifically programmed. Our sections stay on. We like to cook the
slides overnight.
Bernice

Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tom McNemar
Sent: Friday, January 04, 2008 6:43 AM
To: jfish <@t> gladstone.ucsf.edu; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Pressure cooker question

I used to use a pressure cooker but found it to be very harsh.  I had a lot
of trouble getting the sections to stay on the slide.  For the last several
years I'v use a $40 Black and Decker rice steamer.  Slower than the pressure
cooker but it always works and no problems with the sections.  You might
also look into microwave retrieval.  I tried it a few times and it worked
fine.

I steam them for 20 minutes with a 20 minute cooling afterward.  That gives
me plenty of time to make up my buffer and set up my run.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Jo Dee Fish
Sent: Thursday, January 03, 2008 3:26 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Pressure cooker question


We are searching for a cheap but reliable way to do antigen retrieval.  Is
anyone out there using an electric pressure cooker?  And, are the ordinary
household appliances working for you?
Thanks in advance for your help.
Jo Dee
 

~~Jo Dee Fish~~
Research Technologist III
Gladstone Institute of Cardiovascular Disease

Telephone: (415) 734-2567
Fax: (415) 355-0824
E-mail:  <mailto:jfish <@t> gladstone.ucsf.edu> jfish <@t> gladstone.ucsf.edu

Mailing address:  
The J. David Gladstone Institutes
1650 Owens Street
San Francisco, CA 94158

 
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