[Histonet] Bone Marrow Issues

[Rathborne, Toni]

We fix ours in zinc-formalin and do not have this problem.

Toni

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Metzger,
Kenneth
Sent: Friday, February 29, 2008 11:00 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Bone Marrow Issues


We are seeing an artifact that causes loss of nuclear detail, particularly in erythroid cells, giving an appearance of being blown up with no nuclear detail. It is worse in decaled cores. This appears when we fix the cores and clot in formalin. We were using Z-5 and did not have this issue. My Pathologist wants to stick to formalin. None of our other tissue has this issue. Has anyone had this happen? Any help would be appreciated.

Ken


Ken Metzger HTL(ASCP)
Histology Supervisor
ARUP Laboratories
500 Chipeta way
Salt Lake City, UT 84108
801.583.2787 ext 3101


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