[Histonet] hb crystals

[Mark Tarango]

Are you hitting them on the head?

On Wed, Feb 27, 2008 at 6:41 PM, Jim Manavis <jim.manavis <@t> imvs.sa.gov.au>
wrote:

> Dear Sir or Madam:
>
>
>
> I am a PhD student studying intracerebral haemorrhage in the rat and have
> found numerous crystals contained within the haemorrhage, which I presume
> are haemoglobin crystals, although it would be nice to demonstrate this.
> Pearl's stain doesn't work, presumably because the iron is ferrous and
> bound
> tightly, and Lillie's method with ferricyanide and HCL likewise doesn't
> work, presumably because the iron is bound. Our standard DAB technique for
> immunohistochemistry doesn't seem to react either. How might I best
> determine whether or not the crystals are haemoglobin crystals?
>
>
>
> Yours Sincerely,
>
>
>
> Tim Kleinig
>
>
>
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