[Histonet] MMA and tape

[Nancy W. Troiano]

I regularly use clear packing tape with MMA on my more difficult sections 
(e.g. mouse mandibles with teeth).  Just cut to size of block face leaving 
tab of tape on bottom, rub tape gently on block face to adhere tape to 
block, moisten knife with 70% ethanol and pull tape very gently upward 
while microtome is cutting downward.  Use chrome alum gel coated slides and 
put in 37 deg oven for two nights (or 60 deg oven for one nite if not doing 
enzyme stains).  Soak slides in toluene to remove tape, deplastify and 
stain.  Hope this helps.