[Histonet] What is your experience with Histo-Clear II

[Douglas D Deltour]

Sue,

I have been trying the xylene substitutes also. I have tried about ten
different brands and types of clearing reagent. First off, I can tell you
that none of them are superior to xylene. After that realization I can tell
you that the clearing agent that I chose was Citra-Clear from StatLab. It
seems to clear better and faster than the other alternative clearing
reagents (even Histo-Clear). If you are not fond of the citrus odor then the
runner-up is Formula 83 from CBG Biotech. After months of testing, these
were the top two alternative clearing reagents IMO. They are two different
types clearing reagents though. Citra-Clear is citrus based (D-Limonene) and
Formula 83 is a naphthenic hydrocarbon blend. Naphtha is refined petroleum
fractions.  

Douglas D. Deltour HT(ASCP)
Histology Manager
Professional Pathology Services, PC
One Science Court
Suite 200
Columbia, SC 29203
Office (803)252-1913
Fax (803)254-3262
Doug <@t> ppspath.com 
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Breckenridge, Sue
Sent: Wednesday, February 20, 2008 9:47 AM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] What is your experience with Histo-Clear II

I was hoping that any users (past or present) of the clearing agent
Histo-Clear II would be willing to give me feedback on how they liked
it. (For example, did it process tissues on par with other xylene
substitutes; Is there no adverse effect on Immunohistochemistry
staining; Can it be used for glass coverslipping & if so do the slides
dry in a reasonable amount of time). Thank-you for taking the time to
respond!

Sue Breckenridge, Histology Supervisor

Cape Regional Medical Center

Cape May Court House, NJ

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