[Histonet] Technovit 9100 problems

[Barbara Verstraeten]

Dear all,

My name is Barbara Verstraeten and I'm a first yeat phD student at the  
university of Ghent, Belgium.
I work on very small zebrafish (40 hours post fertilisation 'till 6  
days post fertilisation).
I want to do immunostaining on them so I embedded them in technovit  
9100. Everything worked fine but now I have to cut them and put them  
on slides.

My questions:
* What sort of knife do I use?
* I tried to cut them at 3 µm, but they roll up or fall apart. Hints?
* I put my sections on super frost slides. Here we coat them with  
poly-L-lysine.
Does anyone have other suggestions on coating slides so that de  
sections will stick very good?

Thank you very much!

Barbara Verstraeten, phD
Lab Ann Huysseune
Ghent, Belgium
mail:barbara.verstraeten <@t> ugent.be