[Histonet] trichrome and immunohistochemistry

[Walters, Katherine S]

I have done this with DAB and Masson's trichrome.  The DAB turns
purplish, but once you get used to the color change, it works pretty
well.  I left out the hematoxylin, so I guess it's a bichrome.

Kathy Walters  

Katherine Walters 
Histology Director 
Central Microscopy Research Facility 
University of Iowa 
85 Eckstein Medical Research Building 
Iowa City, Iowa 52242-1101 
phone:  (319) 335-8142 
fax: (319) 384-4469 
katherine-walters <@t> uiowa.edu 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Liz
Chlipala
Sent: Friday, February 08, 2008 1:50 PM
To: Bernard Martin; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] trichrome and immunohistochemistry

As long as you use DAB as the chromogen you should be fine, have not
done the trichrome, but have ran other special stains on top of IHC such
as iron, AFB, etc.  I'm not sure how it will look, you might need to
modify the trichrome a bit.

Liz 

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com
 
Ship to Address:
 
Premier Laboratory, LLC
University of Colorado at Boulder
MCDB, Room A3B40
Boulder, CO 80309

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bernard
Martin
Sent: Friday, February 08, 2008 12:45 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] trichrome and immunohistochemistry

Has anyone ever counterstained IHC with trichrome, or heard of it being
done?  Is this even possible? Thanks!


 
________________________________________________________________________
____________
Be a better friend, newshound, and
know-it-all with Yahoo! Mobile.  Try it now.
http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ 


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet