[Histonet] macrophage staining

[Kathy Cormier]

Hi Melissa,


We use Caltag # mf48105 rat ab on mouse FFPE tissue w/ great sucess. 
(primary at 1:100, secondary at 1:100, strepav fitc at 1:100) (HIER ph6 
first though)

Kathy Cormier
Div Comp Med
MIT
----- Original Message ----- 
From: "Melissa Mazan" <melissa.mazan <@t> tufts.edu>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, February 08, 2008 1:00 PM
Subject: [Histonet] macrophage staining


> Hi all,
> I'm trying to do fluorescent immunostaining of macrophages on FFPE mouse 
> tissues - I have tried the BD monoclonal Mac-3 (raised in rat) - it works 
> well with the Vector ABC system, but cannot get it to work well with 
> fluorescence.  Tried a simple primary, 594 conjugated secondary approach - 
> no signal.  Then tried a biotinylated secondary followed with 
> streptavidin - get a messy, non-uniform signal.  Does anyone have an 
> antibody and protocol for fluorescent labeling of macrophages on formalin 
> fixed paraffin embedded tissue? Many thanks - Melissa
>
>
> -- 
> Melissa R. Mazan, DVM, Diplomate ACVIM
> Associate Professor and Director of Sports Medicine
> Tufts Cummings School of Veterinary Medicine
> 200 Westboro Road
> North Grafton, MA 01536
> 508-887-4589
>
>
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