[Histonet] Cracking of paraffin blocks

Rene J Buesa rjbuesa <@t> yahoo.com
Wed Feb 6 08:19:03 CST 2008


That type of cracking ussually is caused by an extremely cold "cooling plate" in the embedding center. Have that area at -5ºC the most,
  René J.

"Wilson, Carol" <wilson_c <@t> ricerca.com> wrote:
  Hi All,

Recently we have seen an increase in cracks of paraffin blocks when we
take them out of the molds. Sometimes it does not affect the tissue,
but there are times that the tissue cracks right along with the
paraffin. We are using Surgipath infiltration on our processor and
Surgipath embedding media in our embedding center. We use reusable
metal base molds. Don't know if would matter or not, but we are
processing animal tissue. We take temperatures daily of all wax baths..
nothing out of the ordinary. Any suggestions would be greatly
appreciated. 

Thanks,

Carol



Carol Wilson

Team Leader - Histology

Ricerca Biosciences, LLC



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