[Histonet] re: good NF-kB antibody

Carl Hobbs carl.hobbs <@t> kcl.ac.uk
Sat Feb 2 15:00:46 CST 2008


I am currently testing out a SCruz mouse anti NFkB Ab.......

I tested it on a mouse transgenic over-expressor of NFKB.....nothing, 
NUCLEAR!
However, in the trans. model there was a lot of lymphocytic infilrate that 
was +++ for this Ab, cytoplasmic......sure , there was a plasma cell 
positivity that I  subsequently realised was due to MOM.
NOTHING Nuclear, sigh,
Sure, I used a wt mouse spleen as a control.
A difficult new area for pwax sections
Carlos 




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