[Histonet] HP staining

[Weems, Joyce]

Oops - pressed send too soon... This was from Dick Dapson back in 2001. I had gotten some Alcian Yellow from Sigma - stain was beautiful, but our pathologists preferred silver.. We do the Genta.

	To all of you who use Alcian yellow:

	Alcian yellow is no longer made, and probably will not be made again because the starting material for its synthesis 	is not available either.  We have been working hard to find an alternative that is indistinguishable from Alcian 	yellow, and are please to report that we have some promising leads.  We need another 6 months or so to conduct 	shelf-life studies.  In the meantime, you can conserve your dye (if you make up your own solutions) by using only 	0.2% instead of the usually prescribed 1.0 %.  The dye simply is not very soluble in water, and a 0.2% solution (0.2 	g per 100 ml) actually has about as much dissolved dye as one made with 1.0 g per 100 ml.


Good luck! j
	




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, February 01, 2008 5:59 PM
To: Martin, Gary; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] HP staining

What is he concerned about: the bacteria or the mucin? I find it a rather bizarre request.
  René J.

"Martin, Gary" <gmartin <@t> marshallmedical.org> wrote:
  My pathologist is asking me to find a rapid stain for helicobacter pylori that stains the mucin yellow. 

Thanks in advance

Gary

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