[Histonet] blood smear

[Valeria Berno]

Hi,

As always I will really appreciate if someone could give me some advice...

I need to stain peripheral blood with anti-GFP antibody and look with
immunofluorescence ("red" seeondary antibody).

My question is about the protocol:
Is it better blood smear, methanol fixation and antibody staining or is it
better to perform staining in the tube and then cytospin?

Could you refer me to a protocol?

thanks in advance

Valeria

Berno Valeria, PhD

EMBL- Mouse Biology Unit
Campus A.  Buzzati-Traverso
Via Ramarini,  32
00015, Monterotondo Scalo  (RM)
Italy

Tel:  +39 06 90091287
Fax: +39 06 90091406
email: valeria.berno <@t> embl.it
www.embl.it