[Histonet] Does anyone know how to achieve a perfect Masson Trichrome stain. Our counterstain (light green in acetic acid) works well but the intensity of the muscle stain is not very bright. Does anyone have any suggestions? We will be staining liver

[pruegg <@t> ihctech.net]

   i  have a protocol from Bryan Hewlett that is called "the fail pro   Masons Trichrome"

   Patsy



   -------- Original Message --------
   Subject: RE: [Histonet] Does    Masson  Trichrome  stain.  Our  counter   acid)
   works well but the intensity of the m   Does anyone have any suggestions? We wil   From: "Lee & Peggy Wenk" <lpwenk <@t> sbcglobal.ne   Date: Tue, December 30, 2008 3:20 pm
   To: "'SARAH REEVES'" <S   <histonet <@t> lists.utsouthwestern.edu>
   Lots of questions, as this is one of those stains that has a lot of p   laces
   that need to be tweaked:
   NEW OR OLD? Is this a new problem    had
   this problem?
   MORDANT: Are   eliminate  this   picric
   acid in the l   RED  DYE:  What dye(s) are you using fo   Please
   include name and color index (CI) n   UK.
   Also,  have  you  switched  recently  t   purchased a
   replacement when you ran out? Wh   dry
   powder?
   PMA/PTA:  Are  you   acid? How
   old is it?
   ONE OR TWO STEP: Just to make certain, is this a true Masson trichrom   e,
   where the red dye is applied, and then the PTA/PMA, and then finally    the
   green  dye? Or is this the traditional Gomori, where the red and gree   n dyes
   and the PTA/PMA are all in one solution?
   TIME  IN  RED AND G   in the
   red  dye  and  the   Each
   different  type  o   tissue, will
   pick up red   TEMPERATURE:  Are the dyes a   That can
   have an influence.
      MACHINE  OR  HAND:  Are you hand staining, where you have control over
   the   amount of time in each solution, where you can check the progress with
      microscope,  where you have a choice of dyes? Or are you staining with
      Or on a machine?
   Sorry,  need  some  more information, before   the
   problem.
   Peggy A. Wenk, HTL(ASCP)SL   Beaumont Hospital
   Royal Oak, MI 48073
   -----Original Message-   From: histonet-bounces <@t> lists.utsouthwestern.edu
   [[1]mailto:histonet-bounces <@t> lists.utsouthwestern.edu]  On  Behalf O   SARAH REEVES
   Sent: Tuesday, December 30, 2008 10:09 AM
   To: histonet   Subject: [Histonet] Does anyone know how to ac   Trichrome  stain. Our counterstain (light green in   well
   but  the  intensity  of  the  muscle  stain is not ve   anyone have
   any suggestions? We will be staining liver b   Does anyone know how to achieve a perfect Masson Trichrome stain.   counterstain  (light  green in acetic acid) works well but the intens   ity of
   the  muscle  stain  is  not very bright. Does anyone have any suggest   ions? We
   will  be  staining liver biopsies mostly in the near future and w   to
   improve our technique.
   Thanks in advance
   Sarah
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