[Histonet] Buffered formalin solution

[Rene J Buesa]

The stock solution in DW with time tends to acidify and if acid the fixation is less effcetive, that is the use of the buffer, to prevent the acidification.
René J.

--- On Tue, 12/30/08, Vlig, Marcel <mvlig <@t> burns.nl> wrote:

From: Vlig, Marcel <mvlig <@t> burns.nl>
Subject: [Histonet] Buffered formalin solution
To: histonet <@t> lists.utsouthwestern.edu
Date: Tuesday, December 30, 2008, 11:21 AM

LS,

 

Recently we discovered that our 37% formalin stock-solution was diluted
in ddH2O instead of PBS. Can this affect the fixation properties of
formalin, we do not see that much effect on our IHC stainings, but I
like to be sure that there are no bad side-effects. Does anyone haven
experience with this?

 

Kind regards,

 

Marcel

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