AW: [Histonet] Does anyone know how to achieve a perfect Masson Trichrome stain. Our counterstain (light green in acetic acid) works well but the intensity of the muscle stain is not very bright. Does anyone have any suggestions? We will be staining liver

[Gudrun Lang]

The Masson-Trichrome has the PMA/PTA step between acid fuchsin and
anilinblue (or light green). You can enhance the brightness of "red", when
you prolong the incubation in PMA/PTA.
Try some protocolls with 5-20 min. The brightness of "red" can also be a
matter due to the washing step after the dyes. Take acidified water and
don't wash too long in diluted ethanol.
I prefer anilinblue because the contrast is better.

If you do a one-step Trichrom stain with the PMA/PTA step before the
dye-mixture, the incubation in the acid should be shorter for "more red" and
longer for "more blue".

Gudrun Lang

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von SARAH
REEVES
Gesendet: Dienstag, 30. Dezember 2008 16:09
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] Does anyone know how to achieve a perfect Masson
Trichrome stain. Our counterstain (light green in acetic acid) works well
but the intensity of the muscle stain is not very bright. Does anyone have
any suggestions? We will be staining liver bio

Does anyone know how to achieve a perfect Masson Trichrome stain. Our
counterstain (light green in acetic acid) works well but the intensity of
the muscle stain is not very bright. Does anyone have any suggestions? We
will be staining liver biopsies mostly in the near future and wish to
improve our technique.

Thanks in advance

Sarah


**********************************************************************
This email and any files transmitted with it are confidential and intended
solely for the use of the individual or entity to whom they are addressed.
Any views or opinions presented are solely those of the author and do 
not necessarily represent those of East Kent Hospitals University NHS Trust.
If
you are not the intended recipient, be advised that you have received
this email in error and that any use, dissemination, forwarding, printing
or copying of this email is strictly prohibited.

If you have received this email in error please notify the system
manager at the following email address: root.postmaster <@t> ekht.nhs.uk

www.kentandmedway.nhs.uk

This footnote also confirms that although this email message has been
swept by MIMEsweeper for the presence of computer viruses, it is strongly 
recommended that you carry out your own virus scan of this
message and any attachments.

www.mimesweeper.com
**********************************************************************


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet