[Histonet] RE:antigen retrieval
Rene J Buesa
rjbuesa <@t> yahoo.com
Sat Dec 27 09:14:41 CST 2008
There is not such a thing as the "best" method. Microwave, pressure cooker and steamer (the one I prefer) depends on the epitope you are trying to detect and your general protocol.
You have to select a method that is good for all your purposes, trying to have different HIER methods for different epitopes is complicated and unpractical for a high volume diagnostic laboratory.
As to the solution it should be a buffer and its pH will depend on the epitope you are trying to detect.
Go to the DAKO web site and you will find there good methods and protocols.
--- On Sat, 12/27/08, anjan kumar <drvet_anjan <@t> hotmail.com> wrote:
From: anjan kumar <drvet_anjan <@t> hotmail.com>
Subject: [Histonet] RE:antigen retrieval
To: "triple immunohistochem" <histonet <@t> lists.utsouthwestern.edu>
Date: Saturday, December 27, 2008, 3:06 AM
can any one tell me which is the best method used for HIER.
MICROWAVE OVEN OR PRESSURE COOKER METHOD.
What kind of solutions should we use for epitope retrieval?
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