[Histonet] RE:antigen retrieval

[Rene J Buesa]

There is not such a thing as the "best" method. Microwave, pressure cooker and steamer (the one I prefer) depends on the epitope you are trying to detect and your general protocol.
You have to select a method that is good for all your purposes, trying to have different HIER methods for different epitopes is complicated and unpractical for a high volume diagnostic laboratory.
As to the solution it should be a buffer and its pH will depend on the epitope you are trying to detect.
Go to the DAKO web site and you will find there good methods and protocols.
René J.

--- On Sat, 12/27/08, anjan kumar <drvet_anjan <@t> hotmail.com> wrote:

From: anjan kumar <drvet_anjan <@t> hotmail.com>
Subject: [Histonet] RE:antigen retrieval
To: "triple immunohistochem" <histonet <@t> lists.utsouthwestern.edu>
Date: Saturday, December 27, 2008, 3:06 AM

hi,
   can any one tell me which is the best method used for HIER.
MICROWAVE OVEN OR PRESSURE COOKER METHOD.

What kind of solutions should we use for epitope retrieval?


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