[Histonet] Re: Understained my slides with hematoxylin
Ade Tunde
atunde90 <@t> yahoo.com
Wed Dec 24 18:48:32 CST 2008
Hi
H&E Stain is very important in the Diagnosis. understaining can be caused by many factors, check the age of your hematoxylin or the concentration of your differentiating solution.
Then to restain the stained slide .place the stained slide in 1% acid alcohol for 2-3minutes and rinse well in distilled water.
then restain in hematoxylin for the required time.You should be fine .
Tunde Ajibade BS, HTL (ASCP)
Histology lab
Newark beth Israel Medical Center.
Newark, NJ
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Subject: Histonet Digest, Vol 61, Issue 35
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Today's Topics:
1. Understained my slides with hematoxylin (Haviland,Joie)
2. charcot-leyden crystals (Horn, Hazel V)
3. Re: Full Time Permanent Day Shift Histotech Position in Fort
Wayne, IN (Mark Tarango)
4. RE: Understained my slides with hematoxylin (Tony Henwood)
5. merry christmas (Michelle Perrins)
6. Charcot-Leyden crystals (alan taylor)
7. Re: Understained my slides with hematoxylin (Peter Carroll)
8. TUNEL on fixed brain tissue (Guillermo Palchik)
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Message: 1
Date: Tue, 23 Dec 2008 14:23:19 -0600
From: "Haviland,Joie" <jhaviland <@t> mdanderson.org>
Subject: [Histonet] Understained my slides with hematoxylin
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <C576A4D7.1C3D%jhaviland <@t> mdanderson.org>
Content-Type: text/plain; charset="iso-8859-1"
Hello:
I was doing H&E's and it was pointed out that I had understained the hematoxylin. But the eosin was ok. How does one go back and restain the slides and remove the old stain? Or do you use a differentiating solution?
Thanks for your help
Happy Holidays
Joie
------------------------------
Message: 2
Date: Tue, 23 Dec 2008 14:31:45 -0600
From: "Horn, Hazel V" <HornHV <@t> archildrens.org>
Subject: [Histonet] charcot-leyden crystals
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<9AE8AA9E1F644B4AA6C155FB6FD51C6317D82F66 <@t> EMAIL.archildrens.org>
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Can someone tell me the best stain to use for Charcot Leyden crystals?
We are looking for them in feces. Thanks.
Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
800 Marshall Slot 820
Little Rock, AR 72202
phone 501.364.4240
fax 501.364.3155
visit us on the web at: www.archildrens.org
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Message: 3
Date: Tue, 23 Dec 2008 12:40:23 -0800
From: "Mark Tarango" <marktarango <@t> gmail.com>
Subject: Re: [Histonet] Full Time Permanent Day Shift Histotech
Position in Fort Wayne, IN
To: "Melissa Phelan" <melissafphelan <@t> gmail.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<5b6eb13e0812231240n60750e7as5cb3978580d8dc6b <@t> mail.gmail.com>
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I work with a tech that you, Melissa, said was getting $1,000 after her
placement. After five months, multiple phone calls, and letter writing she
only got $500. You wouldn't get back to her.
Mark
On Mon, Dec 22, 2008 at 7:47 AM, Melissa Phelan <melissafphelan <@t> gmail.com>wrote:
> Hello,
>
>
>
> My name is Melissa Phelan and I am the* *Director of Lab/Biotech
> recruitment
> here at Healthcare Scouts. My company *specializes* in the *
> permanent/direct* placement of laboratory professionals like you. We are
> currently partnered with numerous labs, and hospitals to recruit for
> their *full
> time/fully befitted* lab and biotech positions nationwide. I have a
> position
> in *Fort Wayne, IN*, and it would be a great fit for you and that you
> uniquely qualify for. I would like to give you the specifics on this
> position, and I would also like to talk with you in more detail on exactly
> what you are looking for, so I can help you find the best opportunity for
> you. I would also like to give you the opportunity to earn our $1000
> referral bonus, if we place someone that you know in a position, we would
> pay you up to $1000 for referring us to that qualified candidate. You can
> reach me at any time at 800.708.0605, ext 139 and ask for Melissa.
>
>
> --
> Melissa Phelan
>
> Laboratory and Biotech
>
> Phone: 800.708.0605 ext. 139
>
> Cell: 407-697-1175
>
> UCF KNIGHTS!!
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------------------------------
Message: 4
Date: Wed, 24 Dec 2008 09:21:50 +1100
From: "Tony Henwood" <AnthonyH <@t> chw.edu.au>
Subject: RE: [Histonet] Understained my slides with hematoxylin
To: "Haviland,Joie" <jhaviland <@t> mdanderson.org>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <B9EAF61856077F47BF9BE2F89AFC555202FB01A7 <@t> hedwig.nch.kids>
Content-Type: text/plain; charset="us-ascii"
Joe,
I would make sure all the mountant is removed. A good soak in xylene and
then a rinse in alcohol, if the mountant has not fully disappeared, you
will see a white opacity on the section.
Rinse in water and then re-stain as usual. The eosin will tend to leach
out in the water rinses and the differentiation solution (after the
haematoxylin - if you use it). Check the sections after blueing.. If it
looks too weak, increase the Hx staining time &/or decrease the
differentiation step. Counterstain with eosin as usual and you should be
right.
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Haviland,Joie
Sent: Wednesday, 24 December 2008 7:23 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Understained my slides with hematoxylin
Hello:
I was doing H&E's and it was pointed out that I had understained the
hematoxylin. But the eosin was ok. How does one go back and restain the
slides and remove the old stain? Or do you use a differentiating
solution?
Thanks for your help
Happy Holidays
Joie
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Message: 5
Date: Wed, 24 Dec 2008 08:36:07 +0200
From: "Michelle Perrins" <Michelle.Perrins <@t> uct.ac.za>
Subject: [Histonet] merry christmas
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4951F476.A704.0070.0 <@t> uct.ac.za>
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To all histonetters around the world, may you have a safe and happy festive holiday..
Best wishes for 2009.
Regards
Michelle from down south in Africa.
Michelle Perrins
Chief Medical Technologist
Forensic Pathology Services
Division Forensic Medicine
Faculty Health Sciences
University of Cape Town
tel: +27 21 406 6001
fax: +27 21 448 1249
Email: Michelle.Perrins <@t> uct.ac.za
------------------------------
Message: 6
Date: Wed, 24 Dec 2008 14:01:51 -0000
From: "alan taylor" <aj.taylor <@t> blueyonder.co.uk>
Subject: [Histonet] Charcot-Leyden crystals
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <76E8F29215084951BAA9CDA93FDD2544 <@t> merlin>
Content-Type: text/plain; charset="iso-8859-1"
Hazel
Charcot-Leyden crystals are frequent findings in faecal samples and sputum submitted for microscopical study, the crystals are easily demonstrated,if present, by making a simple 'wet' prep and adding a drop of Gram's Iodine under the coverslip.
Charcot-Leyden crystals in faecal submissions should lead the investigator to seek the presence of parasitic infection from particularly helminthiasis. Note that Charcot-Leyden crystals are virtually absent from samples of bacillary dysentery, but are frequently found in samples of faeces containing the various amoeba spp. Several other intestinal parasitic worm species result in the presence of Charcot-Leyden crystals.
Within sputum Charcot-Leyden crystals are found in cases submitted from bronchial asthma sufferers, the crystals are often found within Curschmann's spirals that are a feature of this disease. Lung diseases such as Paragonimus ringeri & P westermani , typically found in the region of the Phillipines and Japan, are fluke diseases that result in the presence of Charcot-Leyden crystals from the haemoptic sputum.. The crystals have also been found in pus from amoebic liver abcesses that have found their route of discharge through the lungs.
Hope this info is helpful to you.
A very happy Christmas & New Year to everybody out there in Histoland.
Alan Taylor
Microtechnical Services
Exeter
Devon
England
------------------------------
Message: 7
Date: Wed, 24 Dec 2008 09:34:40 -0500
From: Peter Carroll <carrolpb <@t> umdnj.edu>
Subject: Re: [Histonet] Understained my slides with hematoxylin
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <49524880.2060806 <@t> umdnj.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
> The eosin will tend to leach out in the water rinses and the
differentiation solution
a touch of acid alcohol doesnt hurt either :)
------------------------------
Message: 8
Date: Wed, 24 Dec 2008 10:05:31 -0500
From: Guillermo Palchik <gp62 <@t> georgetown.edu>
Subject: [Histonet] TUNEL on fixed brain tissue
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <7814E917-472D-4179-8AF7-C946D4F2F609 <@t> georgetown.edu>
Content-Type: text/plain; charset=US-ASCII; format=flowed
Dear Histologists,
Does anyone have a good protocol for doing TUNEL on fixed brain slides?
Thanks
Guillermo Palchik
gp62 <@t> georgetown.edu
------------------------------
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