[Histonet] Understained my slides with hematoxylin

Tony Henwood AnthonyH <@t> chw.edu.au
Tue Dec 23 16:21:50 CST 2008


I would make sure all the mountant is removed. A good soak in xylene and
then a rinse in alcohol, if the mountant has not fully disappeared, you
will see a white opacity on the section.

Rinse in water and then re-stain as usual. The eosin will tend to leach
out in the water rinses and the differentiation solution (after the
haematoxylin - if you use it). Check the sections after blueing. If it
looks too weak, increase the Hx staining time &/or decrease the
differentiation step. Counterstain with eosin as usual and you should be


Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Sent: Wednesday, 24 December 2008 7:23 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Understained my slides with hematoxylin

I was doing H&E's and it was pointed out that I had understained the
hematoxylin. But the eosin was ok. How does one go back and restain the
slides and remove the old stain?  Or do you use a differentiating

Thanks for your help

Happy Holidays

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