[Histonet] Isopentane

[Joanne Mauger]

In my experience, liquid nitrogen makes a lot of freezing artifact in
muscle tissue. I have found no better substitue for isopentane if you
want quality morphology in you specimen. We store it in an explosion
proof cabinet.
Jo Mauger

>>> "Tony Henwood" <AnthonyH <@t> chw.edu.au> 12/16/08 6:19 PM >>>
Try liquid nitrogen

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Dana
Settembre
Sent: Wednesday, 17 December 2008 5:27 AM
To: carmen loiselle; pathology education
Subject: Re: [Histonet] Isopentane


Hello Carmen,
Sorry I have no alternative.  I know most labs use that.
When I worked in a neuropathology lab the isopentane was kept in the
refrigerator - an explosion proof one. I believe that means there's no
lightbulb inside and no inner working of a lightbulb inside either.


Dana Settembre, HT ASCP
Immunohistochemistry Lab
UMDNJ - University Hospital
Newark, NJ    USA


>>> carmen loiselle <carmen_loiselle <@t> hotmail.com> 12/16/08 12:24 PM
>>>

Hello ,
 
I currently work in a neuromuscular lab and all muscle biopsies
received
should be cut frozen, no formalin fixed tissues.  My concern though,
is
to work with isopentane , a well known excellent heat conductor but
also
it has the bad reputation of being very explosive. 
In fact , I've been told that few years ago, a lab close by of where I
work , did explode because of this solution.  Is there , in your
knowledge, any other chemical we can use as replacement and that give
as
good results.
 
Any input will be greatly appreciated 
 
Merry XMas to all and happy New Year 
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