[Histonet] at a troubleshooting loss

[Aaron Rose]

This may be a silly suggestion but Have you been filtering your
hematoxylin?  I have seen the sediments that form over time.  I'm not
sure if this could be the dark regions you're seeing but I figured I'd
throw it out there.

-Aaron

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tony
Henwood
Sent: Tuesday, December 16, 2008 3:18 PM
To: Michele Wich; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] at a troubleshooting loss

?carbon from lead pensils used for labelling slides.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Michele
Wich
Sent: Wednesday, 17 December 2008 4:31 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] at a troubleshooting loss


A couple questions for those who are troubleshooting savvy: Other than
inadequate fixation, water being introduced during processing, or too
much heat, what can cause fuzzy, uneven hematoxylin staining with poor
nuclear detail? 

And secondly, aside from formalin, are there any other common agents of
blackish pigment in H & E staining? (The artifact appears on the slides
both on and around the tissues.) 

Any feedback is GREATLY appreciated.

 


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